Search results for "haploid"

Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.)

2016

Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, pl…

Does haplodiploidy purge inbreeding depression in rotifer populations?

2009

Background Inbreeding depression is an important evolutionary factor, particularly when new habitats are colonized by few individuals. Then, inbreeding depression by drift could favour the establishment of later immigrants because their hybrid offspring would enjoy higher fitness. Rotifers are the only major zooplanktonic group where information on inbreeding depression is still critically scarce, despite the fact that in cyclical parthenogenetic rotifers males are haploid and could purge deleterious recessive alleles, thereby decreasing inbreeding depression. Methodology/Principal Findings We studied the effects of inbreeding in two populations of the cyclical parthenogenetic rotifer Brach…

Kinetics of the early development of uniparental human haploid embryos

2016

Objective: To describe morphokinetically the early development of human haploid parthenotes and androgenotes and to compare them with euploid embryos. Design: Experimental study of kinetics. Setting: University-affiliated private fertility center. Patient(s): Experimental haploid parthenotes and androgenotes. Intervention(s): Kinetic study of early development (up to eight cells) of 8 parthenotes, 10 androgenotes, and 20 euploid embryos. Main Outcome Measure(s): Timing of the first seven cleavages determined according to embryo origin, then calculation of the duration of the second and third cell cycles (cc2 and cc3) of whole embryos and individual cells. Result(s): Parthenotes and androgen…

STUDY ON ISOLATED MICROSPORE CULTURE IN CITRUS SINENSIS L. OSBECK CV. MORO, A BLOOD ORANGE CULTIVAR

2014

In vitro tissue culture represents a useful support for the advancement of Citrus breeding and propagation. Haploidy technology, that is the single-step development of complete homozygous genotypes from heterozygous parents through gametic embryogenesis, has already a huge impact on many relevant crops, representing an integral part in their breeding programmes (Germanà 2011a; 2011b). In order to increase the number of genotypes responding to gametic embryogenesis, in vitro isolated microspore culture of Citrus sinensis L. Osbeck cv. Moro, has been carried out, investigating the influence of the culture medium composition, and, particularly, of the plant growth regulator types and concentra…

Anther culture for haploid and doubled haploid production From the issue entitled "Special Issue: In Vitro Ploidy Manipulation in the Genomics Era"

2011

Predictive Factors for Outcome of First Allogeneic Transplant for Elderly Patients With Acute Lymphoblastic Leukemia

2021

Abstract Introduction/Background: The treatment of acute lymphoblastic leukemia (ALL) in patients older than 70 is extremely challenging with dismal outcome. Allogeneic stem cell transplantation (alloHCT) has seen many advancements in the last decades showing benefits in younger ALL patients, but this treatment modality is decreasingly used with increasing age due to high treatment-related mortality. Patients and Methods: We identified 84 ALL patients 70 to 84 years old allografted In 2002 to 2019 from a matched related (23%), unrelated (58%), haploidentical (17%), or cord blood (2%) donor at EBMT participating centers with a median follow-up of 23 months. Results: The 2-year relapse incide…

Impacto de la alorreactividad NK en el trasplante haploidéntico de progenitores hematopoyéticos asociado al uso de ciclofosfamida postrasplante

2022

Introducción: El trasplante haploidéntico de progenitores hematopoyéticos (haplo-TPH) es una opción terapéutica, cuando no se dispone de donante HLA-idéntico. Sin embargo, existe controversia sobre qué factores son los que tienen implicación pronóstica en la selección del donante. Objetivos: Identificar factores relevantes para escoger el mejor donante haploidéntico. Para ello se analizaron aspectos clínicos e inmunológicos. Dentro de los distintos modelos de predicción de la alorreactividad NK, este estudio pretende conocer cuál aporta mayor información. Material y método: Estudio prospectivo multicéntrico de 113 parejas donante-receptor (D/R). Los pacientes, con diversas patologías, tras …

PSCDGs of mouse multipotent adult germline stem cells can enter and progress through meiosis to form haploid male germ cells in vitro

2010

Spermatogonial stem cells (SSCs) provide the basis for spermatogenesis throughout adult life by undergoing self-renewal and differentiation into sperm. SSC-derived cell lines called multipotent adult germline stem cells (maGSCs) were recently shown to be pluripotent and to have the same potential as embryonic stem cells (ESCs). In a differentiation protocol using retinoic acid (RA) and based on a double selection strategy, we have shown that ESCs are able to undergo meiosis and produce haploid male germ cells in vitro. Using this differentiation protocol we have now succeeded to generate haploid male germ cells from maGSCs in vitro. maGSCs derived from a Stra8-EGFP transgenic mouse line wer…

First stages of microsporere programming to embryogenesis through anther culture in Prunus armeniaca L.

2011

6 páginas, 3 figuras, 2 tablas -- PAGS nros. 152-157

Anther culture in Citrus clementina: a way to regenerate tri-haploids

2005

Abstract. Regenerants from anther culture of Citrus clementina Hort. ex Tan. cvv. Nules, SRA 63, and Monreal were obtained in different experiments from 1994 to 2002. Genetic analysis of 37 such regenerants was carried out using 4 microsatellite markers that were heterozygous in the parental genotypes. The results showed that in all cases but one the regenerants carried only one or the other allele of the parental genotype, and were therefore homozygous and produced through a process of gametophytic embryogenesis. Ploidy analysis by flow cytometry of 94 regenerants showed that as many as 82% of them were tri-haploids, rather than haploids or doubled-haploids as expected, with other ploidy …