Search results for "holography"

Dispersion management in two-photon microscopy by using diffractive optical elements.

2013

We demonstrate efficient generation of wide-field fluorescence signals in two-photon microscopy exploiting diffractive optical elements and short pulses by using a dispersion-compensated beam delivery optics module. Computer-generated holograms are codified onto a phase-only spatial light modulator, which allows for arbitrary single-shot patterning of the sample. Spatiotemporal shaping of the pulse is mandatory to overcome spatial chirp and pulse-front tilt effects that spread both in space and time the irradiance patterns, thus limiting not only the spatial resolution but also the signal-to-noise ratio in two-photon microscopy. By using a multipass amplifier delivering 30 fs, 0.8 mJ pulses…

Dynamical binary modulation of ultrabroadband light beams by using principal states of polarization of liquid crystal devices

2009

Dynamical modulation of ultrabroadband beams, such as those produced by femtosecond laser or incoherent sources, is not an easy task due to the dispersive nature of the devices commonly employed. Phase modulation has been performed by means of deformable micromirror arrays. These devices are expensive and do not permit amplitude modulation. For micro- and nano-structuring of materials with femtosecond lasers is common to pattern the surface with the light irradiance produced by a computer generated hologram implemented onto a liquid crystal (LC) type spatial light modulator. This enables dynamical patterning [1,2]. Reduced spectral bandwidths, of the order of tens of nanometers, have been u…

Optoelectronic Information Encryption with Incoherent Light

2005

Characterization of the Microshell Surface Using Holography

2011

AbstractTo characterize the shape, the quality, and the roughness of microshells, digital holographic microscopy technology is used because it offers an appropriate ability to these studies. It captures holograms to reconstruct a double image, one for the intensity and another one for the phase. Using rotation axis, bump counting for the complete microshell surface is possible with a very high speed. Using image stitching and three-dimensional surface rebuilding software, mapping can be done in a few minutes. Each bump can then be characterized on the map by its position, diameter, and height.

Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging.

2010

Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The pr…

Synthetic aperture superresolution with multiple off-axis holograms

2006

An optical setup to achieve superresolution in microscopy using holographic recording is presented. The technique is based on off-axis illumination of the object and a simple optical image processing stage after the imaging system for the interferometric recording process. The superresolution effect can be obtained either in one step by combining a spatial multiplexing process and an incoherent addition of different holograms or it can be implemented sequentially. Each hologram holds the information of each different frequency bandpass of the object spectrum. We have optically implemented the approach for a low-numerical-aperture commercial microscope objective. The system is simple and rob…

Holography and Superresolution

2013

The capability of improving the spatial resolution of imaging systems is usually known as superresolution. Some methods provide improve resolution by playing with the imaging part of the system and without modifying the optical parameters of the imaging lenses. And others act over the geometry, shape and size of sampling pixels in the detection array. The former strategy allows optical superresolution while the latter provide geometrical superresolution. In this contribution, we will review the state of the art in optical superresolution approaches understood as the possibility to overcome the limited resolving power of imaging systems beyond the bounds imposed by Abbe's diffraction theory.…

Surpassing digital holography limits by lensless object scanning holography.

2012

We present lensless object scanning holography (LOSH) as a fully lensless method, capable of improving image quality in reflective digital Fourier holography, by means of an extremely simplified experimental setup. LOSH is based on the recording and digital post-processing of a set of digital lensless holograms and results in a synthetic image with improved resolution, field of view (FOV), signal-to-noise ratio (SNR), and depth of field (DOF). The superresolution (SR) effect arises from the generation of a synthetic aperture (SA) based on the linear movement of the inspected object. The same scanning principle enlarges the object FOV. SNR enhancement is achieved by speckle suppression and c…

Remote monitoring of building oscillation modes by means of real-time Mid Infrared Digital Holography

2016

AbstractNon-destructive measurements of deformations are a quite common application of holography but due to the intrinsic limits in the interferometric technique, those are generally confined only to small targets and in controlled environment. Here we present an advanced technique, based on Mid Infrared Digital Holography (MIR DH), which works in outdoor conditions and provides remote and real-time information on the oscillation modes of large engineering structures. Thanks to the long wavelength of the laser radiation, large areas of buildings can be simultaneously mapped with sub-micrometric resolution in terms of their amplitude and frequency oscillation modes providing all the modal p…

Synthetic aperture microscopy using off-axis illumination and polarization coding

2007

A new method to improve the resolution of optical imaging systems beyond the classical Rayleigh resolution limit is presented. The technique relies on synthetic aperture generation in three stages. The first one (encoding stage) uses an illumination procedure that combines both on-axis and off-axis illumination beams with different polarization states onto the object. After the imaging system, a second stage (decoding stage) allows the recovering of the encoded spatial-frequency object information by means of an interferometric configuration based on the polarization coding carried out in the previous stage. Finally, a third stage (digital post-processing stage) is used to generate a synthe…