Search results for "hrom"
showing 10 items of 11836 documents
Influence of Riverine Input on Norwegian Coastal Systems
2020
Coastal ecosystems are of high ecological and socioeconomic importance and are strongly influenced by processes from land, sea, and human activities. In this study, we present physical, chemical, and biological observations over two consecutive years from three study regions along the Norwegian coast that represent a broad latitudinal gradient in catchment and oceanographic conditions (∼59–69°N): outer Oslofjord/southern Norway, Runde/western Norway, and Malangen/northern Norway. The observations included river monitoring, coastal monitoring, and sensor-equipped ships of opportunity (“FerryBox”). The riverine discharge and transports were an order of magnitude higher, and the spatiotemporal…
New insight into the colonization processes of common voles: inferences from molecular and fossil evidence.
2008
Biologie et Gestion des Populations, Campus International de Baillarguet, Montferrier/Lez, FranceElucidating the colonization processes associated with Quaternary climatic cycles is important in order to understand the distribution of biodiversity and the evolutionary potential of temperate plant and animal species. In Europe, general evolutionary scenarios have been defined from genetic evidence. Recently, these scenarios have been challenged with genetic as well as fossil data. The origins of the modern distributions of most temperate plant and animal species could predate the Last Glacial Maximum. The glacial survival of such populations may have occurred in either southern (Mediterranea…
Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the gamma-decalactone production by Yarrowia lipolytica.
2000
International audience; Size of methyl ricinoleate droplets during biotransformation into gamma-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d32 of 2.5 microm whereas it was 0.7 microm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 microm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions.
Lipid composition of the vacuolar membrane of Acer pseudoplatanus cultured cells
1993
Tonoplast was prepared by osmotic lysis of a pure vacuolar fraction isolated from protoplasts derived from Acer pseudoplatanus cultured cells. After their extraction, neutral and polar lipids were separated by a thin layer chromatography. Phospholipids, glycolipids and neutral lipids represented 44.5%, 39.1% and 16.4% of total lipids, respectively. Sterols (glycosylated plus non-glycosylated forms) constituted 30.8% of total lipids; 75% of sterols were glycosylated. The most prominent lipids were phosphatidylethanolamine (20.8%), phosphatidylcholine (13.5%), ceramide monohexoside (12.8%), steryl glycoside (12.2%) and acylated steryl glycoside (10.9%). Glucose was the only sugar released by …
Use of chlorophyll fluorescence induction kinetics to study translocation and detoxication of DCMU-type herbicides in plant leaves
1984
Transient levels of the fluorescence induction rise were used to quantify partial photosynthesis inhibition by DCMU -type herbicides in whole leaves. Assays in different crop or weed species showed a good accuracy in measurements (generally, variation was lower than 5%). This technique was applied to the problem of varietal selectivity of wheat towards chlortoluron.
Doubled haploid production in onion (Allium cepa L.): from gynogenesis to chromosome doubling
2020
Bulb onion (Allium cepa L.) is an allogamous diploid (2n = 16) important for its culinary uses, nutritional value, and medicinal benefits. Despite its economic importance, onion yields and bulb quality are declining, emphasizing the need for new and improved strategies for maintaining and enhancing overall crop quality. Development of inbred lines in onion through traditional breeding is often difficult due to its biennial life cycle, inbreeding depression, and comparatively high heterozygosities. Moreover, genetic research in onion has been hampered by large nuclear genome size. In this regard, gynogenic doubled haploids promise several advantages over inbred lines in support of onion bree…
Fast pyrolysis of hot-water-extracted and soda-AQ-delignified okra (Abelmoschus esculentus) and miscanthus (miscanthus x giganteus) stalks by Py-GC/MS
2018
Abstract The thermochemical behavior of various samples of okra ( Abelmoschus esculentus ) and miscanthus ( Miscanthus x giganteus ) stalks (initial, hot-water-extracted, and those from sulfur-free delignification) were studied by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). In all cases, major GC-amenable condensable products were measured semi-quantitatively and classified into several product groups. The formation of these product groups from different feedstock samples with varying mass portions of their structural constituents (carbohydrates and lignin) was investigated at 500 °C and 700 °C with a residence time of 5 s and 20 s. The main product groups were aliphatic comp…
Effect of Enzyme Amounts Used in Gastrointestinal Digestion Upon Solubility and Caco-2 Cell Uptake Assays of Minerals from Infant Formulas
2005
The effect of enzyme amounts used in gastrointestinal in vitro digestion upon the solubility and Caco-2 cell uptake of calcium, iron and zinc from infant formulas (IFs) was studied. Different amounts of enzymes (g enzyme/g IF), pepsin (0.002 and 0.048), pancreatin (0.0005, 0.002 and 0.01) and bile extract (0.003, 0.125 and 0.0625) were assayed. Mineral soluble contents and mineral uptakes by Caco-2 cells were affected by the enzyme amounts used in digestion. Although the highest mineral solubility (Ca 98.6 vs 46.2%; Fe 98.1 vs 83.9%; Zn 98.4 vs 83%) was obtained when the lowest enzyme (pepsin 0.002 vs 0.048; pancreatin 0.0005 vs 0.01g/g IF) and bile extract (0.003 vs 0.0625g/g IF) amounts …
Improvement of Analytical Conditions of Mineral Caco-2 Cell Uptake Assays
2004
Caco-2 cell mineral uptake assays are used to estimate the bioavailability of minerals from foods. The uptake of minerals by Caco-2 cells can be affected by several factors – particularly the conditions of the in vitro digestion process and the growth conditions of the cell culture. Therefore, a standardisation of the assays conditions is required to obtain reproducible results. This work determined the effect of enzyme demineralisation, the inactivation of the proteolytic activity of the digest and the replacement of distilled-deionised water by cell culture degree water. Treatment with Chelex-100 resin was applied to remove calcium, iron and zinc from pepsin and pancreatin-bile salts. Th…
Aspartic proteinase from barley grains is related to mammalian lysosomal cathepsin D
1991
Resting barley (Hordeum vulgare L.) grains contain acid-proteinase activity. The corresponding enzyme was purified from grain extracts by affinity chromatography on a pepstatin-Sepharose column. The pH optimum of the affinity-purified enzyme was between 3.5 and 3.9 as measured by hemoglobin hydrolysis and the enzymatic activity was completely inhibited by pepstatin a specific inhibitor of aspartic proteinases (EC 3.4.23). Further purification on a Mono S column followed by activity measurements and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the affinity-purified enzyme preparation contained two active heterodimeric aspartic proteinases: a larger 48k Da enzyme, c…