Search results for "iHEP"
showing 10 items of 16 documents
Duck Hepatitis B Virus Requires Cholesterol for Endosomal Escape during Virus Entry
2008
ABSTRACT The identity and functionality of biological membranes are determined by cooperative interaction between their lipid and protein constituents. Cholesterol is an important structural lipid that modulates fluidity of biological membranes favoring the formation of detergent-resistant microdomains. In the present study, we evaluated the functional role of cholesterol and lipid rafts for entry of hepatitis B viruses into hepatocytes. We show that the duck hepatitis B virus (DHBV) attaches predominantly to detergent-soluble domains on the plasma membrane. Cholesterol depletion from host membranes and thus disruption of rafts does not affect DHBV infection. In contrast, depletion of chole…
Erweiterte Hemihepatektomie links mit Rekonstruktion der rechten Lebervene bei intrahepatischem cholangiozellulärem Karzinom
2016
Die erweiterte Hemihepatektomie links mit der Resektion der Lebersegmente 1, 2, 3, 4 a/b, 5 und 8 ist eine auch weiterhin sehr herausfordernde Leberresektion, deren Indikation bei gut selektionierten Patienten die Chance auf eine kurative Resektion bei primaren oder sekundaren Lebertumoren mit komplizierter Lage bieten kann. Die Morbiditat und Mortalitat ist gegenuber den nicht erweiterten Leberresektionen erhoht. Eine prazise Bildgebung und genaue Operationsplanung unter Berucksichtigung des verbleibenden Lebervolumens sowie von Einflussfaktoren wie Vorschadigungen des Leberparenchyms durch Chemotherapie oder eine Fibrose/Zirrhose ist hierbei unabdingbar. Notwendig wird dieses operative Vo…
The Southampton Consensus Guidelines for Laparoscopic Liver Surgery: From Indication to Implementation
2018
OBJECTIVE: The European Guidelines Meeting on Laparoscopic Liver Surgery was held in Southampton on February 10 and 11, 2017 with the aim of presenting and validating clinical practice guidelines for laparoscopic liver surgery.BACKGROUND: The exponential growth of laparoscopic liver surgery in recent years mandates the development of clinical practice guidelines to direct the speciality's continued safe progression and dissemination.METHODS: A unique approach to the development of clinical guidelines was adopted. Three well-validated methods were integrated: the Scottish Intercollegiate Guidelines Network methodology for the assessment of evidence and development of guideline statements; th…
Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
2019
Abstract Background Human fibroblasts can be reprogrammed into induced hepatocyte-like cells through the expression of a set of transcription factors. Although the generation of induced hepatocyte-like cells by HNF4A, HNF1A, and FOXA3 expression has proven to be a robust experimental strategy, using multiple lentivirus results in a highly variable heterogeneous population. Methods We designed and implemented a novel approach based on the delivery of reprogramming factors and green fluorescent protein in a single doxycycline-inducible lentiviral vector using 2A self-cleaving peptides. Results Fibroblasts infected with the lentiviral vector can be amplified in basic fibroblast culture media i…
Broad Spectrum Thiopeptide Recognition Specificity of theStreptomyces lividans TipAL Protein and Its Role in Regulating Gene Expression
1999
Microbial metabolites isolated in screening programs for their ability to activate transcription of the tipA promoter (ptipA) in Streptomyces lividans define a class of cyclic thiopeptide antibiotics having dehydroalanine side chains ("tails"). Here we show that such compounds of heterogeneous primary structure (representatives tested: thiostrepton, nosiheptide, berninamycin, promothiocin) are all recognized by TipAS and TipAL, two in-frame translation products of the tipA gene. The N-terminal helix-turn-helix DNA binding motif of TipAL is homologous to the MerR family of transcriptional activators, while the C terminus forms a novel ligand-binding domain. ptipA inducers formed irreversible…
CCDC 1814648: Experimental Crystal Structure Determination
2018
Related Article: George Papamokos, Jakob Wudarczyk, Robert Graf, Dieter Schollmeyer, Martin Baumgarten, Klaus Müllen, George Floudas|2018|Macromolecules|51|3330|doi:10.1021/acs.macromol.8b00215
CCDC 1814647: Experimental Crystal Structure Determination
2018
Related Article: George Papamokos, Jakob Wudarczyk, Robert Graf, Dieter Schollmeyer, Martin Baumgarten, Klaus Müllen, George Floudas|2018|Macromolecules|51|3330|doi:10.1021/acs.macromol.8b00215
CCDC 1814646: Experimental Crystal Structure Determination
2018
Related Article: George Papamokos, Jakob Wudarczyk, Robert Graf, Dieter Schollmeyer, Martin Baumgarten, Klaus Müllen, George Floudas|2018|Macromolecules|51|3330|doi:10.1021/acs.macromol.8b00215