Search results for "idia"

Clostridium difficile IStron CdISt1: Discovery of a Variant Encoding Two Complete Transposase-Like Proteins

2004

ABSTRACT Screening a Clostridium difficile strain collection for the chimeric element Cd ISt1 , we identified two additional variants, designated Cd ISt1 -0 and Cd ISt1 -III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known Cd ISt1 variants led us to define four types of IStrons that we designated Cd ISt1 -0 through Cd ISt1 -III. Since Cd ISt1 -0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element.

Molecular typing of clinical Candida strains using random amplified polymorphic DNA and contour-clamped homogenous electric fields electrophoresis.

2009

Aims:  This report describes an investigation into the genetic profiles of 38 Candida albicans and 19 Candida glabrata strains collected from a dental hospital of Monastir (Tunisia) and the Laboratory of Parasitology, Farhat Hached Hospital of Sousse (Tunisia), using two typing methods: random amplified polymorphic DNA (RAPD) and contour-clamped homogenous electric fields (CHEF). Methods and Results:  The two methods (RAPD and CHEF electrophoresis) were able to identify clonal-related isolates from different patients. RAPD method using two primers (CA1 and CA2) exhibited the highest discriminatory power by discriminating 22 genotypes for C. albicans with CA1 oligonucleotides and 19 genotype…

Planar Cell Polarity Signaling in Collective Cell Movements During Morphogenesis and Disease

2012

Collective and directed cell movements are crucial for diverse developmental processes in the animal kingdom, but they are also involved in wound repair and disease. During these processes groups of cells are oriented within the tissue plane, which is referred to as planar cell polarity (PCP). This requires a tight regulation that is in part conducted by the PCP pathway. Although this pathway was initially characterized in flies, subsequent studies in vertebrates revealed a set of conserved core factors but also effector molecules and signal modulators, which build the fundamental PCP machinery. The PCP pathway in Drosophila regulates several developmental processes involving collective cel…

Genetics of Polyglandular Failure

2010

Publisher Summary This chapter discusses the genetic aspects of polyglandular failure syndromes (APS).The polyglandular failure syndromes comprise of a wide spectrum of autoimmune disorders and encompass a rare juvenile type (APS1) and more frequent adult types (APS2 and APS3). The juvenile type APS1 is also known as autoimmune polyendocrinopathy candidiasis ectodermal dystrophy because it consists of three main disorders, namely chronic mucocutaneous candidiasis, autoimmune hypoparathyroidism and autoimmune Addison's disease. APS2 is defined as the association between Addison's disease and either autoimmune thyroid disease or type 1 diabetes mellitus and APS3 is characterized by the presen…

Ag-NOR and C-banding analysis of spermatocyte chromosomes ofClavelina lepadiformis(Ascidiacea, Aplousobranchiata)

1991

SUMMARYChromosome number n = 9 and 2n = 18 for Clavelina lepadiformis (Ascidiacea, Aplousobranchiata) from the Gulf of Palermo have been determined. Silver staining analysis of testicular cells reveals that within-individual variability in NOR banding patterns is present. Using the C-banding procedure, a very impressive heterochromatin amount seems to characterize the chromosome set of this species.

Comparison of morphotypic and genotypic methods for strain delineation inCandida

1998

Summary. We compared two phenotypic methods, colony morphotyping on Sabouraud-tripheniltetrazolium agar (STTZ) and serotyping, with two genotypic methods, karyotyping and Random Amplified Polymorphic DNA bands obtained by PCR amplification (RAPD-PCR), for strain delineation in 33 Candida clinical isolates and two C. albicans strains from culture collections. Analysis of isolates on STTZ showed 11 different morphotypes. In two patients there was a switch in the morphotype coincidential with a change in the susceptibility of the isolates to azole antifungals. C. albicans isolates were divided into two serotypes. Sixteen and 18 different patterns were identified among the Candida isolates by k…

Neogeographic technologies as a tool for reducing the disaster risk: a testimonies’ map during the 2020 lockdown in Italy

2023

This paper illustrates a geographical and multidisciplinary research titled "My lived space" and conducted in Italy, during the lockdown period due to the pandemic of Covid-19. The research aimed at using geospatial platforms to collect and map discursive, visual and oral testimonies, stories and narratives of individuals. It means, exploring the various testimonies of people related to quarantine, physical distancing, resistances and experiences, and practices that occur in the daily spaces of their homes and in local communities.

Dagli anni ruggenti dell’«Ora». I dibattiti sul Gattopardo

2019

Il saggio ricostruisce il dibattito che si svolse, sapientemente orchestrato dal suo memorabile Direttore Vittorio Nisticò, sul giornale "L'Ora", subito dopo la pubblicazione del "Gattopardo". A porre le basi della discussione è Leonardo Sciascia la cui polemica si appunta sul modo di leggere la storia della Sicilia per poi vedere avvicendarsi le voci di scrittori giornalisti intellettuali. Ne risulta la vitalità culturale di un quotidiano che seppe coniugare denuncia e cultura. Tale linea editoriale viene ulteriormente confermata dal secondo dibattito che si svolse a dieci anni della pubblicazione del romanzo. A fare un bilancio sul successo furono chiamati insieme a Sciascia, che attenua …

Effects of hormonal contraception on vaginal flora, can contraceptive use lead to vulvovaginal candidiasis (VVC) or bacterial vaginosis (BV)

2016

Molecular evidence for the presence of cryptic evolutionary lineages in the freshwater copepod genus Hemidiaptomus G.O. Sars, 1903 (Calanoida, Diapto…

2010

The pattern of morphological and mtDNA cytochrome b diversity of three calanoid copepod species belonging to the diaptomid genus Hemidiaptomus has been investigated with the aim of checking the reliability of the morphological characters currently used for species identification, and the possible presence of cryptic taxa. A sharply different molecular structuring has been observed in the studied species: while Hemidiaptomus amblyodon exhibits a remarkable constancy throughout the European range of its distribution area (maximum inter-populations cytochrome b divergence of 3%), observed distances between presumed conspecific lineages of Hemidiaptomus gurneyi (maximum divergence of 21.5%) and…