Search results for "in utero"

showing 9 items of 49 documents

Valutazione dell'attività dell'asse ipotalamo-ipofisi-surrene, in seguito all'esposizione in utero a stress acuto e cronico nella progenie di ratto a…

2011

asse ipotalamo-ipofisi-surreneSettore BIO/14 - Farmacologiaprogenie di ratto adulta.esposizione in utero a stress acuto e cronico
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Non-cell autonomous and non-catalytic activities of ATX in the developing brain

2015

The intricate formation of the cerebral cortex requires a well-coordinated series of events, which are regulated at the level of cell-autonomous and non-cell autonomous mechanisms. Whereas cell-autonomous mechanisms that regulate cortical development are well-studied, the non cell-autonomous mechanisms remain poorly understood. A non-biased screen allowed us to identify Autotaxin (ATX) as a non cell-autonomous regulator of neural stem cell proliferation. ATX (also known as ENPP2) is best known to catalyze lysophosphatidic acid (LPA) production. Our results demonstrate that ATX affects the localization and adhesion of neuronal progenitors in a cell autonomous and non-cell autonomous manner, …

autotaxinChemistryCortical developmentGeneral Neuroscienceradial gliaRegulatorin utero electroporationNeural stem cellNeuronal stem celllcsh:RC321-571LPAin utero electroporation.chemistry.chemical_compoundmedicine.anatomical_structureCerebral cortexLysophosphatidic acidmedicineOriginal Research ArticleNon catalyticAutotaxinProgenitor cellGeneNeurosciencelcsh:Neurosciences. Biological psychiatry. NeuropsychiatryNeuroscienceFrontiers in Neuroscience
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Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆

2013

Background Fetal-to-neonatal transition is associated with oxidative stress. In preterm infants, immaturity of the antioxidant system favours supplemental oxygen-derived morbidity and mortality. Objectives To assess if prolonging in utero-like oxygenation during the fetal-to-neonatal transition limits oxidative stress in the lung and brain, improving postnatal adaptation of mice pups. Material and methods Inspiratory oxygen fraction (FiO2) in pregnant mice was reduced from 21% (room air) to 14% (hypoxia) 8–12 h prior to delivery and reset to 21% 6–8 h after birth. The control group was kept at 21% during the procedure. Reduced (GSH) and oxidized (GSSG) glutathione and its precursors [γ-glut…

gsr (glutathione reductase gene)pgd phosphogluconate dehydrogenase geneGPX1FiO2 inspiratory oxygen fractionγ-GC (gamma-glutamyl cysteine)PhysiologyBiochemistryMice0302 clinical medicinePregnancyquinone oxidoreductase 1) [noq1 (NAD(P)H]NAD(P)H Dehydrogenase (Quinone)gapdh glyceraldehyde-3-phosphate dehydrogenase geneP7 1 week after birthGSH (reduced glutathione)Oxidoreductases Acting on Sulfur Group Donorsme1 (malic enzyme 1 gene)glutathioneLungSpO2 oxygen saturationlcsh:QH301-705.5γ-GC–NEM gamma-glutamyl cysteine covalently bonded to N-ethylmaleimidechemistry.chemical_classification0303 health sciencesGSSG oxidized glutathioneGlutathione peroxidaseO14 (hypoxia group FiO2=14%)Brainm/z mass-to-charge ratioG18 18th day of gestationCell Hypoxia3. Good healthpgd (phosphogluconate dehydrogenase gene)In uterogclm glutamylcysteine ligase modifier subunit genesrnx1 sulfiredoxin 1 genelcsh:Medicine (General)me1 malic enzyme 1 genesrnx1 (sulfiredoxin 1 gene)gclm (glutamylcysteine ligase modifier subunit gene)γ-GC–NEM (gamma-glutamyl cysteine covalently bonded to N-ethylmaleimide)trxnd1 (thioredoxin reductase 1 gene)redox regulation03 medical and health sciencesnoq1 NAD(P)H:quinone oxidoreductase 1γ-GC gamma-glutamyl cysteineCySH L-cysteinePregnancyg6pdx (glucose 6 phosphate dehydrogenase gene)GlutathioneOxygenationgapdh (glyceraldehyde-3-phosphate dehydrogenase gene)medicine.diseaseMice Inbred C57BLOxygenP1 24 h after birthGCL glutamylcysteine ligasechemistryOxidative stressRedox regulationNEM (N-ethylmaleimide)O14 hypoxia group (FiO2=14%)GSH reduced glutathioneClinical Biochemistrymedicine.disease_causechemistry.chemical_compoundGlutathione Peroxidase GPX1GS–NEM reduced glutathione covalently bonded to N-ethylmaleimideSpO2 (oxygen saturation)oxidative stressg6pdx glucose 6 phosphate dehydrogenase genelcsh:R5-920GSSG (oxidized glutathione)G18 (18th day of gestation)gsr glutathione reductase geneGlutathionegpx1 glutathione peroxidase 1 genemedicine.anatomical_structurem/z (mass-to-charge ratio)LC–MS/MS (liquid chromatography coupled to tandem mass spectrometry)FemaleLC–MS/MS liquid chromatography coupled to tandem mass spectrometryO21 (normoxia group FiO2=21%)paO2 (partial pressure of oxygen)gpx1 (glutathione peroxidase 1 gene)Research Papernoq1 (NAD(P)H:quinone oxidoreductase 1)CySH (l-cysteine)FiO2 (inspiratory oxygen fraction)CyS–NEM (cysteine covalently bonded to N-ethylmaleimide)030225 pediatricsmedicineP7 (1 week after birth)AnimalsGCL (glutamylcysteine ligase)P1 (24 h after birth)O21 normoxia group (FiO2=21%)CyS–NEM cysteine covalently bonded to N-ethylmaleimide030304 developmental biologyGlutathione PeroxidaseLungOrganic ChemistryGS–NEM (reduced glutathione covalently bonded to N-ethylmaleimide)trxnd1 thioredoxin reductase 1 geneMolecular biologypaO2 partial pressure of oxygenAnimals NewbornGene Expression Regulationlcsh:Biology (General)NEM N-ethylmaleimidefetal-to-neonatal transitionoxygenOxidative stressFetal-to-neonatal transition
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Prenatal Mouth Movements: Can We Identify Co-Ordinated Fetal Mouth and LIP Actions Necessary for Feeding?

2012

Observations of prenatal movement patterns of mouth and lips essential for feeding could have the potential for an assessment of the readiness to feed after birth. Although there is some research on suckingper se, we know very little about prenatal preparatory movements for sucking, namely, the ability to co-ordinate opening the mouth widely and then pursing the lips as if around a teat or nipplein utero. The purpose of the present study was to test two hypotheses using an adapted version of the Facial Action Coding Scheme: first that mouth stretch (AU 27) will be followed by lip pucker (AU 18), and second that these coordinated movement patterns will increase as a function of gestational a…

medicine.medical_specialtyArticle Subject[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition03 medical and health sciences0302 clinical medicinePhysical medicine and rehabilitationstomatognathic system030225 pediatricsmedicineCoordinated movementMouth movementsFetus030219 obstetrics & reproductive medicinebusiness.industrylcsh:RJ1-570Gestational agelcsh:PediatricsFetal ageSurgeryFetal mouthIn uteroPediatrics Perinatology and Child Healthbusiness[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition4d ultrasoundResearch ArticleInternational Journal of Pediatrics
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Uterine Receptivity and the Ramifications of Ovarian Stimulation on Endometrial Function

2007

Controlled ovarian stimulation (COS) is widely used in assisted reproduction techniques (ART). However, hormonal treatment induces endometrial alterations that may alter implantation rates compared with natural cycles. Endometrial alterations have been observed by histological and biochemical techniques. The recent developments in functional genomics have provided objective tools to analyze the endometrium in natural cycles and evaluate the impact of COS protocols in endometrial development. This article describes the fundamental aspects of endometrial receptivity in natural cycles and reports how COS affects the morphology, biochemistry, and the genomic pattern of the endometrium.

medicine.medical_specialtyEndocrinology Diabetes and Metabolismmedia_common.quotation_subjectUterusOvaryStimulationBiologyEndometriumAndrologyEndometriumEndocrinologyOvulation InductionPregnancyPhysiology (medical)Internal medicinemedicineHumansEmbryo ImplantationMenstrual CycleMenstrual cycleOligonucleotide Array Sequence Analysismedia_commonPrincipal Component AnalysisGene Expression ProfilingGene Expression Regulation DevelopmentalObstetrics and GynecologyGenomicsEndocrinologymedicine.anatomical_structureReproductive MedicineIn uteroFemaleFunction (biology)HormoneSeminars in Reproductive Medicine
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Ovarian cysts in the fetus and neonate--changes in sonographic pattern in the follow-up and their management.

1992

In a multicenter trial we retrospectively evaluated the clinical and sonographic data of 49 neonatal ovarian cysts, 44 of which were detected prenatally and 5 on the first day after delivery. Of the 44 prenatally detected cysts 39 were purely cystic, 5 echogenic or had a mixed pattern. In 20 patients the cystic appearance changed during delivery from purely cystic to a mixed pattern being independent on the size of the cyst. 26 of the 44 cysts were treated surgically. Salpingotorsion was found in 8 and was independent on the size of the cyst. In 15 a salpingo-oophorectomy or oophorectomy was performed, in 11 the ovary was saved. 23 patients were followed sonographically: 15 cysts showed com…

medicine.medical_specialtyPercutaneousmedicine.medical_treatmentOvaryUltrasonography PrenatalPregnancyMulticenter trialparasitic diseasesmedicineHumansRadiology Nuclear Medicine and imagingCystNeuroradiologyRetrospective StudiesPregnancybusiness.industryInfant NewbornOophorectomymedicine.diseaseSurgeryFetal DiseasesOvarian Cystsmedicine.anatomical_structureIn uteroPediatrics Perinatology and Child HealthFemalebusinessFollow-Up StudiesPediatric radiology
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Effect of histamine on the longitudinal and circular muscle of the oestrogen dominated rat uterus.

1993

The response of the longitudinal and circular myometrial strips to histamine was studied in oestrogen-treated rats. Histamine produced a dose-related inhibitory response in KCl-contracted longitudinal and circular uterine strips. Histamine was equipotent in producing the relaxant response but the maximal effect achieved in the longitudinal muscle was higher than the circular one. Ranitidine antagonized the histamine-induced relaxation with a similar dose ratio in both longitudinal and circular strips. Clemizole and reserpine treatment did not produce any modification of the dose-response curve to histamine. In the longitudinal and circular strips which were not preconstricted by KCl, neithe…

medicine.medical_specialtyReserpinePyridinesMuscle RelaxationImmunologyUterusBiologyIn Vitro TechniquesToxicologyInhibitory postsynaptic potentialRanitidinePotassium ChlorideRanitidineHistamine Agonistschemistry.chemical_compoundUterine ContractionInternal medicinemedicineAnimalsPharmacology (medical)Receptors Histamine H2Rats WistarPharmacologyUterusEstrogensMuscle SmoothReserpineClemizoleRatsmedicine.anatomical_structureEndocrinologychemistryIn uteroBenzimidazolesFemalemedicine.symptomHistaminemedicine.drugMuscle contractionHistamineAgents and actions
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Regulation by oestrogens of tachykinin NK3 receptor expression in the rat uterus.

1997

The expression of the tachykinin NK3 receptor and its regulation by ovarian steroids were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from ovariectomized rats. A single transcript corresponding to the 325-bp product expected for the tachykinin NK3 receptor was detected in uteri from olive oil-treated (control) ovariectomized rats. The level of tachykinin NK3 receptor mRNA in progesterone-treated animals was similar to that observed in uteri from control ones. Tachykinin NK3 receptor mRNA levels were significantly smaller in uteri from oestrogen-treated ovariectomized rats, with approximately a 32-fold decrease. These findings suggest that oestrogen, but not…

medicine.medical_specialtyanimal structuresNk3 receptorDNA Complementarymedicine.drug_classOvariectomyUterusBiologydigestive systemcomplex mixturesPolymerase Chain ReactionInternal medicineGene expressionmedicineAnimalsRNA MessengerRats WistarProgesteronePharmacologyElectrophoresis Agar GelMessenger RNAurogenital systemmusculoskeletal neural and ocular physiologyUterusEstrogensReceptors Neurokinin-3RatsEndocrinologymedicine.anatomical_structureGene Expression RegulationEstrogenIn uteroRat uterusOvariectomized ratFemaleEuropean journal of pharmacology
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Indagini genetiche e trattamenti prenatali in utero della Sindrome Adreno Genitale (SAG) in una Coorte Siciliana.

2008

La deficienza dell'enzima 21 idrossilasi (21OHD) è la causa più comune di sindrome adrenogenitale (SAG). Il gene implicato è il CYP21 (6p21.3). La SAG è un problema clinico-sociale in quanto nel 95% dei casi genera l'iperandrogenismo dei neonati di sesso femminile. Il trattamento più effettuato in corso di virilizzazzione dei genitali femminili esterni è di tipo chirurgico (genitoplastica). Il trattamento prenatale in utero è una alternativa medica con il fine di compensare il difetto enzimatico e prevenire l'eccesso di androgeni in corso di morfogenesi fetale. Un nuovo protocollo diagnostico-terapeutico prenatale è stato sviluppato sulla base di una terapia precoce (alla 5° WG) con Desamet…

sindrome adrenogenitale Il gene CYP21 trattamento prenatale in utero.Settore MED/38 - Pediatria Generale E Specialistica
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