Search results for "init"

Analysis of LSD in human body fluids and hair samples applying ImmunElute columns.

2000

Immunoaffinity extraction units (LSD ImmunElute) are commercially available for the analysis of lysergic acid diethylamide (LSD) in urine. The ImmunElute resin contains immobilized monoclonal antibodies to LSD. We applied the ImmunElute procedure to serum and also to human hair samples. For hair analysis the samples were first extracted with methanol under sonication. The extracts were then purified using the ImmunElute resin. LSD analysis was carried out with HPLC and fluorescence detection. The immunoaffinity extraction provides highly purified extracts for chromatographic analysis. The limit of detection (signal-to-noise ratio = 3) has been determined to be < 50 pg regardless of which sa…

Advantages of monolithic over particulate columns for multiresidue analysis of organic pollutants by in-tube solid-phase microextraction coupled to c…

2011

Abstract The performance of a monolithic C 18 column (150 mm × 0.2 mm i.d.) for multiresidue organic pollutants analysis by in-tube solid-phase microextraction (IT-SPME)-capillary liquid chromatography has been studied, and the results have been compared with those obtained using a particulate C 18 column (150 mm × 0.5 mm i.d., 5 μm). Chromatographic separation has been carried out under isocratic elution conditions, and for detection and identification of the analytes a UV-diode array detector has been employed. Several compounds of different chemical structure and hydrophobicity have been used as model compounds: simazine, atrazine and terbutylazine (triazines), chlorfenvinphos and chlorp…

Immunochromatographic Assay for Quantitation of Milk Progesterone.

1996

We describe a rapid immunochromatographic method for the quantitation of progesterone in bovine milk. The method is based on a 'competitive' assay format using the monoclonal antibody to progesterone and a progesterone-protein conjugate labelled with colloidal gold particles. The monoclonal antibody to progesterone is immobilized as a narrow detection zone on a porous membrane. The sample is mixed with colloidal gold particles coated with progesterone-protein conjugate, and the mixture is allowed to migrate past the detection zone. Migration is facilitated by capillary forces. The amount of labelled progesterone-protein conjugate bound to the detection zone, as detected by photometric scann…

Separation of plasma membrane proteins of cultured human fibroblasts by affinity chromatography on bonded microparticulate silicas.

1984

Abstract Adsorbents for high-performance affinity chromatography were prepared by bonding proteins and reactive Procion triazine dyes to 3-isothiocyanatopropyl- and 3-aminopropylsilicas. The materials prepared were used successfully in the separation of hydrophobic plasma membrane proteins of cultured human fibroblasts. The data obtained show that the reaction of 3-isothiocyanatopropyltriethoxysilane (ITCPS) with the surface hydroxyl groups of silica yields a new and convenient route to preparing an “activated carrier” that is capable of coupling with potential affinity ligands containing amino functional groups. The reaction and bonding procedures of 3-isothiocyanatopropyltriethoxysilane a…

Weak and strong recognition by 2-way randomized automata

1997

Languages weakly recognized by a Monte Carlo 2-way finite automaton with n states are proved to be strongly recognized by a Monte Carlo 2-way finite automaton with no(n) states. This improves dramatically over the previously known result by M.Karpinski and R.Verbeek [10] which is also nontrivial since these languages can be nonregular [5]. For tally languages the increase in the number of states is proved to be only polynomial, and these languages are regular.

Block-Deterministic Regular Languages

2001

We introduce the notions of blocked, block-marked and blockdeterministic regular expressions. We characterize block-deterministic regular expressions with deterministic Glushkov block automata. The results can be viewed as a generalization of the characterization of one-unambiguous regular expressions with deterministic Glushkov automata. In addition, when a language L has a block-deterministic expression E, we can construct a deterministic finite-state automaton for L that has size linear in the size of E.

Gozos del glorioso Patriarca San Juan de Matha ... del Orden de la Santísima Trinidad, Redencion de Cautivos : dados á luz año 1805, para cantarse en…

El full orlat Grav. xil. enmarcat de "S. Ivan de Matha", flanquejat per gerros amb flors Text del goig a tres col. separades per filets

A snapshot of the transition from monogenetic volcanoes to composite volcanoes: Case study on the Wulanhada Volcanic Field (northern China)

2022

The transition processes from monogenetic volcanoes to composite volcanoes are poorly understood. The Late Pleistocene to Holocene intraplate monogenetic Wulanhada Volcanic Field (WVF) in northern China provides a snapshot of such a transition. Here we present petrographic observations, mineral chemistry, bulk rock major and trace element data, thermobarometry, and a partial melting model for the WVF to evaluate the lithology and partial melting degree of the mantle source, the crystallization conditions, and pre-eruptive magmatic processes occurring within the magma plumbing system. The far-field effect of India–Eurasia collision resulted in a relatively high degree (10 %–20 %) of partial …

Novel group A rotavirus G8 P[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs

2011

International audience; Rotavirus is a worldwide major cause of diarrhea outbreaks in neonatal ruminants. An outbreak of ovine diarrheic syndrome (ODS) in 50-75 days-old lambs (weaned lambs) is described. Fecal immunochromatography and intestinal immunohistochemistry for rotavirus group A were performed. In addition, semi-nested multiplex RT-PCR for G and P rotavirus genotyping in combination with sequencing were performed, to support the diagnosis and identify the viral strain. A novel ovine rotavirus group A G8 P[1] strain was determined as the main cause of the ODS observed, whereas other pathogens were ruled out.

Synthesis of 8-aminomorphans with high KOR affinity

2021

2-Azabicyclo[3.3.1]nonanes (morphans) with a (3,4-dichlorophenyl)acetyl group at 2-position and a pyrrolidino moiety at 8-position were designed as conformationally restricted analogs of piperidine-based KOR agonists. The synthesis started with 4-oxopiperidine-2-carboxylic acid comprising 13 reaction steps. At first the ketone 10 was transformed into diester 7 bearing a propionate side chain. Dieckmann condensation of diester 7 to afford bicyclic enolester 14 and subsequent Krapcho deethoxycarbonylation represent the key steps of the synthesis. The enantiomeric pyrrolidines (1S,5R,8R)-5a and (1R,5S,8S)-5a were separated by chiral HPLC. The eutomer (1S,5R,8R)-5a showed high KOR affinity (K-i…