Search results for "init"
showing 10 items of 6629 documents
Yeast karyopherin Kap95 is required for cell cycle progression at Start
2010
Abstract Background The control of the subcellular localization of cell cycle regulators has emerged as a crucial mechanism in cell division regulation. The active transport of proteins between the nucleus and the cytoplasm is mediated by the transport receptors of the β-karyopherin family. In this work we characterized the terminal phenotype of a mutant strain in β-karyopherin Kap95, a component of the classical nuclear import pathway. Results When KAP95 was inactivated, most cells arrested at the G2/M phase of the cell cycle, which is in agreement with the results observed in mutants in the other components of this pathway. However, a number of cells accumulate at G1, suggesting a novel r…
Differential Regulation of CCL22 Gene Expression in Murine Dendritic Cells and B Cells
2005
Abstract The activated T cell-attracting CC chemokine CCL22 is expressed by stimulated B cells and mature dendritic cells (DC). We have cloned and sequenced the complete mouse gene, including 4 kb of the 5′-flanking promoter region, and detected two distinct sites for initiation of transcription by 5′-RACE. Reporter gene assays indicate that the promoter reflects the specificity of the endogenous gene. Within the proximal promoter region, we identified potential binding sites for NF-κB, Ikaros, and a putative GC box. All three regions bind proteins. The NF-κB site was shown to specifically bind NF-κB subunits p50 and p65 from nuclear extracts of LPS-stimulated B cells, B cell line A20/2J, T…
On Transition Structures for Hydride Transfer Step in Enzyme Catalysis. A Comparative Study on Models of Glutathione Reductase Derived from Semiempir…
1996
As a model of the chemical reactions that take place in the active site of gluthatione reductase, the nature of the molecular mechanism for the hydride transfer step has been characterized by means of accurate quantum chemical characterizations of transition structures. The calculations have been carried out with analytical gradients at AM1 and PM3 semiempirical procedures, ab initio at HF level with 3-21G, 4-31G, 6-31G, and 6-31G basis sets and BP86 and BLYP as density functional methods. The results of this study suggest that the endo relative orientation on the substrate imposed by the active site is optimal in polarizing the C4-Ht bond and situating the system in the neighborhood of the…
Symmetric Galerkin BEM for Non Linear Analysis of Historical Masonries
2015
The preservation of the historical and monumental buildings, but also of the considerable heritage of old constructions made by traditional techniques, is one of the actual problems of the structural mechanics. The level of knowledge of their structural behavior in presence of external actions is made through calculus methods and simple procedures in order to allow a reading of the material suffering degree and as a consequence of the related safety. Unfortunately, often the masonry panels show openings located in an irregular way and cracks having small or big dimensions. In these cases the employment of strategies, as for example the identifying of the masonry piers and the transformation…
A note on finite PST-groups
2007
[EN] A finite group G is said to be a PST-group if, for subgroups H and K of G with H Sylow-permutable in K and K Sylow-permutable in G, it is always the case that H is Sylow-permutable in G. A group G is a T*-group if, for subgroups H and K of G with H normal in K and K normal in G, it is always the case that H is Sylow-permutable in G. In this paper, we show that finite PST-groups and finite T*-groups are one and the same. A new characterisation of soluble PST-groups is also presented.
Fertility and Polarized Cell Growth Depends on eIF5A for Translation of Polyproline-Rich Formins in Saccharomyces cerevisiae
2014
eIF5A is an essential and evolutionary conserved translation elongation factor, which has recently been proposed to be required for the translation of proteins with consecutive prolines. The binding of eIF5A to ribosomes occurs upon its activation by hypusination, a modification that requires spermidine, an essential factor for mammalian fertility that also promotes yeast mating. We show that in response to pheromone, hypusinated eIF5A is required for shmoo formation, localization of polarisome components, induction of cell fusion proteins, and actin assembly in yeast. We also show that eIF5A is required for the translation of Bni1, a proline-rich formin involved in polarized growth during …
Contribution of polyadenylate sequences to the translational efficiency of globin messenger RNAs
1987
mRNAs from reticulocyte polysomes were fractionated by chromatography on poly(U)-Sepharose and thermal elution. The molar ratio of alpha- to beta-globin mRNA was found to be 2:1 and 1:1 respectively in short- and long-poly(A) size classes. Translational analyses indicated that the globin mRNAs containing long poly(A) tracts (with a mean length of about 70 nucleotides) directed protein synthesis with higher rates than did mRNA containing short poly(A) tracts (15-35 nucleotides). Experiments performed with sub-saturating mRNA concentrations showed that the digestion with RNAase H induced a decrease in the translational capacity of both globin mRNAs and an increase in the alpha- to beta-globin…
Masculinité transnationale et corps-mâle dans les romans d’Abdellah Taïa
2016
I romanzi dello scrittore marocchino d'espressione francese Abdellah Taïa esaminano con attenzione la maschilità nel contesto migratorio. Omosessuale ed esiliato, Taïa cerca di costruire la sua identità sessuale secondo il modello occidentale; così facendo, contribuisce alla creazione di una maschilità islamica transnazionale che si decentra, pur restando ancorata all'eredità culturale delle sue terre d'origine. L'articolo si propone di interrogare le modalità di tale costruzione identitaria a partire dagli studi di Connell sulla maschilità egemonica e dalla psicoanalisi lacaniana applicata alla dimensione immaginaria del corpo. The novels of the French Moroccan writer Abdellah Taïa examine…
Advanced Monitoring of Rotor Broken Bar in Double Squirrel Cage Induction Machines Based on Wavelet Analysis
2012
The diagnosis of induction machine faults is commonly realized through Motor Current Signature Analysis (MCSA), i.e. by classical spectrum analysis of the input currents. In case of large or double cage induction motors, rotor broken bars fault detection based on sideband current components may fail due to the presence of inter bar currents that reduce the degree of rotor asymmetry, leading to reduced relevance of these spectral components. But inter bar currents produce core vibrations in the axial direction, which can be detected using vibration analysis techniques, in order to overcome the limits of the classical MCSA for these purposes. In this paper, an advanced use of the Discrete wav…
Transverse instability of periodic and generalized solitary waves for a fifth-order KP model
2017
We consider a fifth-order Kadomtsev-Petviashvili equation which arises as a two-dimensional model in the classical water-wave problem. This equation possesses a family of generalized line solitary waves which decay exponentially to periodic waves at infinity. We prove that these solitary waves are transversely spectrally unstable and that this instability is induced by the transverse instability of the periodic tails. We rely upon a detailed spectral analysis of some suitably chosen linear operators.