Search results for "init"
showing 10 items of 6629 documents
Germinal center B cells govern their own fate via antibody feedback
2013
High-affinity antibodies reenter germinal centers (GCs) and limit antigen access, thus causing sustained directional evolution in GCs toward higher-affinity antibody production.
Binding Properties and Stability of the Ras-Association Domain of Rap1-GTP Interacting Adapter Molecule (RIAM)
2012
The Rap1-GTP interacting adapter protein (RIAM) is an important protein in Rap1-mediated integrin activation. By binding to both Rap1 GTPase and talin, RIAM recruits talin to the cell membrane, thus facilitating talin-dependent integrin activation. In this article, we studied the role of the RIAM Ras-association (RA) and pleckstrin-homology (PH) domains in the interaction with Rap1. We found that the RA domain was sufficient for GTP-dependent interaction with Rap1B, and the addition of the PH domain did not change the binding affinity. We also detected GTP-independent interaction of Rap1B with the N-terminus of RIAM. In addition, we found that the PH domain stabilized the RA domain both in …
Expression of neurotrophins, GDNF, and their receptors in rat thyroid tissue
1999
Levels of mRNA for neurotrophins (brain-derived neurotrophic factor, BDNF; neurotrophin 3, NT-3; neurotrophin 4, NT-4) and their receptors (trkA, trkB, trkC) and for glial cell line-derived neurotrophic factor (GDNF) and its receptors (ret, GDNFR-alpha) were measured in rat thyroid tissue by ribonuclease protection assays. In thyroid tissue the NT-3 mRNA level was threefold lower and the NT-4 mRNA level sixfold higher than those detected in adult rat hippocampus, while BDNF mRNA was undetectable. Very low levels of mRNA for truncated trkB and trkC receptors and no catalytic trkA, trkB or trkC were found. In conclusion NT-3 and NT-4, but not the corresponding functional receptors, are expres…
Photo-DHEA--a functional photoreactive dehydroepiandrosterone (DHEA) analog.
2011
Abstract The steroid hormone dehydroepiandrosterone (DHEA) has beneficial effects on vascular function, survival of neurons, and fatty acid metabolism. However, a specific receptor for DHEA has not been identified to date. Here, we describe the synthesis of a photoreactive DHEA derivative (Photo-DHEA). In Photo-DHEA, typical characteristics of DHEA are conserved: (i) a “planar” tetracyclic ring system with a Δ 5 double bond, (ii) a 3β-hydroxyl group, and (iii) a keto group at C17. In cell-based assays, Photo-DHEA showed the same properties as DHEA. We conclude that Photo-DHEA is suitable for radioiodination to yield a tool for the identification of the elusive DHEA receptor.
Effect of electron-beam irradiation on structure of ultra high molecular weight polyethylene used in medical implants
2004
Określono wpływ napromieniania strumieniem elektronów dawką równoważną stosowanej w praktyce podczas sterylizacji radiacyjnej endoprotez chirurgicznych (26 kGy) oraz dwukrotnie większą (2 . 26 kGy) na zmianę struktury PE-UHMW o nazwie handlowej „,Chirulen 1120”' (tabela 1) służącego do otrzymywania panewek endoprotez biodrowych. Metodą FT-IR zbadano zmiany strukturalne polimeru, stwierdzając głównie występowanie skutków jego utlenienia (wzrost udziału grup karbonylowych typu ketonowego, estrowego i nadestrowego, grup hydroksylowych oraz winylowych) (rys. 1). Metodą GPC stwierdzono, że napromienianie powoduje także częściową degradację makrocząsteczek (zmniejszenie ciężaru cząsteczkowego o j…
Evidence for protein leverage in a general population sample of children and adolescents
2023
Background/ObjectivesThe strong regulation of protein intake can lead to overconsumption of total energy on diets with a low proportion of energy from protein, a process referred to as protein leverage. The protein leverage hypothesis posits that protein leverage explains variation in energy intake and potentially obesity in ecological settings. Here, we tested for protein leverage and the protein leverage hypothesis in children and adolescents.Subjects/MethodsA population sample of children, mean (SD) age 7.6 (0.4) years (n = 422), followed up at age 9.8 (0.4) years (n = 387) and at age 15.8 (0.4) years (n = 229), participating for the Physical Activity and Nutrition in Children (PANIC) st…
A Strategy for the Prediction of the Response of Hysteretic Systems: A Base for Capacity Assessment of Buildings under Seismic Load
2014
A statistical non linearization method is used to approximate systems modeled by the Bouc differential equa- tion and excited by a Gaussian white noise external load. To this aim restricted potential models (RPM) are used, which are suitable for an extended number of nonlinear problems as have been proved several times. Since the solution of RPM is known by the probabilistic point of view, all statistical characteristics can be derived at once with advantages by the computational point of view. Hence, this paper discusses the possibility to determine sets of parameters characterizing po- tential models that are valid for describing a hysteretic behavior. In this way the characterization of …
Summary of Week VII
2018
International audience; Week VII of the INT program 2018 “Probing Nucleons and Nuclei in High Energy Collisions” was dedicated to topics at the interface of the electron-ion collider (EIC), heavy ion and proton-nucleus collisions. The EIC will provide complementary tools to investigate and constrain the initial state in HIC collisions, as well as transport properties of QCD matter which can be extracted from observables that are sensitive to final states interactions such as pt-broadening and energy loss. The contributed talks and discussions covered a variety of physics topics from saturation physics and the origin of multi-particle correlations in HIC to jet quenching and the strong coupl…
Human enterovirus group B viruses rely on vimentin dynamics for efficient processing of viral nonstructural proteins
2020
We report that several viruses from the human enterovirus group B cause massive vimentin rearrangements during lytic infection. Comprehensive studies suggested that viral protein synthesis was triggering the vimentin rearrangements. Blocking the host cell vimentin dynamics with IDPN did not significantly affect the production of progeny viruses and only moderately lowered the synthesis of structural proteins such as VP1. In contrast, the synthesis of the non-structural proteins 2A, 3C, and 3D was drastically lowered. This led to attenuation of the cleavage of the host cell substrates PABP and G3BP1 and reduced caspase activation, thus leading to prolonged cell survival. Furthermore, the loc…
Slow infection due to lowering amount of intact versus empty particles is a characteristic feature of Coxsackievirus B5, dictated by the structural p…
2019
Enterovirus B species typically cause a rapid cytolytic infection leading to efficient release of progeny viruses. However, they are also capable of persistent infections in tissues, which are suggested to contribute to severe chronic states such as myocardial inflammation and type 1 diabetes. In order to understand the factors contributing to differential infection strategies, we constructed a chimera by combining the capsid proteins from a fast cytolysis causing echovirus 1 (EV1) with non-structural proteins from Coxsackievirus B5 (CVB5) showing persistent infection in RD cells. The results showed that the chimera behaved similar to the parental EV1 leading to efficient cytolysis in both …