Search results for "integrity"
showing 10 items of 134 documents
Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio
2020
AbstractThe accumulation of mutations is frequently associated with alterations in gene function leading to the onset of diseases, including cancer. Aiming to find novel genes that contribute to the stability of the genome, we screened the Saccharomyces cerevisiae deletion collection for increased mutator phenotypes. Among the identified genes, we discovered MET7, which encodes folylpolyglutamate synthetase (FPGS), an enzyme that facilitates several folate-dependent reactions including the synthesis of purines, thymidylate (dTMP) and DNA methylation. Here, we found that Met7-deficient strains show elevated mutation rates, but also increased levels of endogenous DNA damage resulting in gross…
Modulation of base excision repair of 8-oxoguanine by the nucleotide sequence.
2013
8-Oxoguanine (8-oxoG) is a major product of oxidative DNA damage, which induces replication errors and interferes with transcription. By varying the position of single 8-oxoG in a functional gene and manipulating the nucleotide sequence surrounding the lesion, we found that the degree of transcriptional inhibition is independent of the distance from the transcription start or the localization within the transcribed or the non-transcribed DNA strand. However, it is strongly dependent on the sequence context and also proportional to cellular expression of 8-oxoguanine DNA glycosylase (OGG1)-demonstrating that transcriptional arrest does not take place at unrepaired 8-oxoG and proving a causal…
Widespread transcriptional gene inactivation initiated by a repair intermediate of 8-oxoguanine.
2016
DNA damage can significantly modulate expression of the affected genes either by direct structural interference with transcription components or as a collateral outcome of cellular repair attempts. Thus, DNA glycosylases of the base excision repair (BER) pathway have been implicated in negative transcriptional response to several spontaneously generated DNA base modifications, including a common oxidative DNA base modification 8-oxoguanine (8-oxoG). Here, we report that single 8-oxoG situated in the non-transcribed DNA strand of a reporter gene has a pronounced negative effect on transcription, driven by promoters of various strength and with different structural properties, including viral…
The catalytic properties of DNA G-quadruplexes rely on their structural integrity
2021
International audience; The influence of the G-quartet structural integrity on the catalytic activity of the G-quadruplex (G4) was investigated by comparing the G4-DNAzyme performances of a series of G4s with a G-vacancy site and a G-triplex (G-tri). The results presented herein not only confirm that the structural integrity of the 3’-end G-quartet is necessary for G4s to be catalytically competent but also show how to remediate G-vacancy-mediated catalytic activity losses via the addition of guanine surrogates in an approach referred to as G-vacancy complementation strategy that is applicable to parallel G4s only. Furthermore, this study demonstrates that the terminal G-quartet could act a…
8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1.
2011
The common DNA base modification 8-oxo-7,8-dihydroguanine (8-oxo-G) affects the efficiency and fidelity of transcription. We constructed plasmid substrates carrying single 8-oxo-G residues, specifically positioned in the transcribed or the non-transcribed DNA strands, to investigate their effects on the expression of an EGFP reporter gene and to explore the role of base excision repair in the mechanism of transcription inhibition. We report that 8-oxo-G does not directly block transcription in cells, since a single 8-oxo-G in the transcribed DNA strand did not reduce the EGFP expression levels in repair-deficient (OGG1-null) mouse embryonic fibroblast cell lines. Rather, inhibition of trans…
Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
2021
International audience; Although relative air humidity (RH) strongly influences microbial survival, its use for fighting surface pathogens in the food industry has been inadequately considered. We asked whether RH control could destroy Listeria monocytogenes EGDe by envelope damage. The impact of dehydration in phosphate-buffered saline (PBS) at 75%, 68%, 43% and 11% RH on the bacterial envelope was investigated using flow cytometry and atomic force microscopy. Changes after rehydration in the protein secondary structure and peptidoglycan were investigated by infrared spectroscopy. Complementary cultivability measurements were performed by running dehydration–rehydration with combinations o…
Juicios y sentencias del Tribunal de la Santa Inquisición de Murcia contra ciudadanos residentes en la Corona de Aragón, desde el siglo XV al XIX
2020
Resumen: Durante cinco siglos, el tribunal de la Inquisicon de Murcia se encargo de juzgar los casos mas diversos y realizar innumerables persecuciones, con mayor o menor intensidad y dureza, contra aquellos que se salian de la doctrina represiva marcada por la iglesia. A los supuestos delitos religiosos contra la doctrina y la fe, se unian aquellos de caracter sexual, ademas de los llevados a cabo contra numerosos clerigos de dudoso comportamiento e integridad etica y moral.Extraidos del catalogo publicado por Juan Blaquez Miguel en la revista Murgetana en el ano 1987, teniendo como fuente principal la documentacion existente en el Archivo Historico Nacional, el estudio que se presenta ana…
On Using Conceptual Modeling for Ontologies
2004
Are database concepts and techniques suitable for ontology design and management? The question has been on the floor for some time already. It gets a new emphasis today, thanks to the focus on ontologies and ontology services due to the spread of web services as a new paradigm for information management. This paper analyzes some of the arguments that are relevant to the debate, in particular the question whether conceptual data models would adequately support the design and use of ontologies. It concludes suggesting a hybrid approach, combining databases and logic-based services.
Trust-enhanced intelligent security model
2012
In this paper we propose a trust-enhancement of access control to protect both integrity and confidentiality based on trustworthiness of users performing operations and documents' content analysis. We propose to utilize trustworthiness opinions from subjective logic and express levels of integrity as levels of trustworthiness. We assign confidentiality levels based on contents of documents and use opinions to express trustworthiness of such assignments.
Ultrascale+ for the new ATLAS calorimeter trigger board dedicated to jet identification
2018
To cope with the expected increase in luminosity at the Large Hadron Collider in 2021, the ATLAS collaboration is planning a major detector upgrade to be installed during Long Shutdown 2. As a part of this, the Level 1 trigger, based on calorimeter data, will be upgraded to exploit the fine granularity readout using a new system of Feature EXtractors (FEXs), which each reconstruct different physics objects for the trigger selection. The Jet FEX (jFEX) is one of three FEXs and has been conceived to identify small/large area jets, large area tau leptons, missing transverse energy and the total sum of the transverse energy. The use of the latest generation Xilinx Field Programmable Gate Array …