6533b86dfe1ef96bd12c9fb4
RESEARCH PRODUCT
The catalytic properties of DNA G-quadruplexes rely on their structural integrity
Mingpan ChengJun ZhouJiawei WangJean-louis MergnyJean-louis MergnyDehui QiuJielin ChenHuangxian JuDavid Monchaudsubject
GuanineG-vacancy02 engineering and technology010402 general chemistryG-quadruplex01 natural sciencesCofactorCatalysischemistry.chemical_compoundNucleotideG-quartet integrity[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM][SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM]chemistry.chemical_classificationbiologyG-quadruplex[CHIM.CATA] Chemical Sciences/CatalysisGeneral Medicine[CHIM.CATA]Chemical Sciences/Catalysis021001 nanoscience & nanotechnology0104 chemical sciencesComplementationGuanine surrogatechemistrybiology.proteinBiophysicsG-quadruplex DNAzyme0210 nano-technologyDNAHemindescription
International audience; The influence of the G-quartet structural integrity on the catalytic activity of the G-quadruplex (G4) was investigated by comparing the G4-DNAzyme performances of a series of G4s with a G-vacancy site and a G-triplex (G-tri). The results presented herein not only confirm that the structural integrity of the 3’-end G-quartet is necessary for G4s to be catalytically competent but also show how to remediate G-vacancy-mediated catalytic activity losses via the addition of guanine surrogates in an approach referred to as G-vacancy complementation strategy that is applicable to parallel G4s only. Furthermore, this study demonstrates that the terminal G-quartet could act as a proximal coordinating group and cooperate with the flanking nucleotide to activate the hemin cofactor.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-07-01 |