Search results for "kr"
showing 10 items of 7011 documents
Metabolic and Functional Genomic Studies Identify Deoxythymidylate Kinase as a target in LKB1 Mutant Lung Cancer
2013
Abstract The LKB1/STK11 tumor suppressor encodes a serine/threonine kinase, which coordinates cell growth, polarity, motility, and metabolism. In non–small cell lung carcinoma, LKB1 is somatically inactivated in 25% to 30% of cases, often concurrently with activating KRAS mutations. Here, we used an integrative approach to define novel therapeutic targets in KRAS-driven LKB1-mutant lung cancers. High-throughput RNA interference screens in lung cancer cell lines from genetically engineered mouse models driven by activated KRAS with or without coincident Lkb1 deletion led to the identification of Dtymk, encoding deoxythymidylate kinase (DTYMK), which catalyzes dTTP biosynthesis, as synthetica…
Blattabacteria, the endosymbionts of cockroaches, have small genome sizes and high genome copy numbers.
2008
Summary Blattabacteria are intracellular endosymbionts of cockroaches and primitive termites that belong to the class Flavobacteria and live only in specialized cells in the abdominal fat body of their hosts. In the present study we determined genome sizes as well as genome copy numbers for the endosymbionts of three cockroach species, Blattella germanica, Periplaneta americana and Blatta orientalis. The sole presence of blattabacteria in the fat body was demonstrated by rRNA-targeting techniques. The genome sizes of the three blattabacteria were determined by pulsed field gel electrophoresis. The resulting total genome sizes for the three symbionts were all approximately 650 15 kb. Compari…
Genetic diversity and phenotypic characterization of Iodobacter limnosediminis associated with skin lesions in freshwater fish
2021
The relatively unknown genus Iodobacter sp. has been repeatedly isolated from skin ulcers and saprolegniosis on freshwater fish in Finland, especially farmed salmonids. Genetic characterization verified that all 23 bacterial isolates studied here belonged to the species Iodobacter limnosediminis, previously undescribed from the fish microbiota. Whole-genome pulsed-field gel electrophoresis revealed variability between the I. limnosediminis strains, suggesting that they were most likely of environmental origin. Two I. limnosediminis strains caused lesions in 27%–53% of brown trout (Salmo trutta) injected intramuscularly (p ≤ .05). The lesions represented moderate to severe tissue damage, but…
Upes nēģa Lampetra fluviatilis populāciju ģenētiskās daudzveidības izpēte ar mikrosatelītiem
2020
Šis bakalaura darbs ir turpinājums kursa darbam - “Kurzemes upēs sastopamo upes nēģu Lampetra fluviatilis mikrosatelītu genotipēšana”. Šis ir pirmais pētījums Latvijā par upes nēģa ģenētisko daudzveidību un populācijas ģenētisko struktūru Latvijas upēs, pamatojoties uz mikrosatelītu DNS variācijām. Darba mērķis bija novērtēt upes nēģa populācijas ģenētisko daudzveidību Latvijā. Iegūtie dati veicinās zināšanas par upes nēģu ģenētisko struktūru un palīdzēs izstrādāt ieteikumus ģenētisko resursu ilgtspējīgai apsaimniekošanai. No piecām upēm (Saka, Venta, Daugava, Salaca, Gauja) kopā tika ievākti 210 upes nēģa audu paraugi no muguras spurām, no kuriem tika izdalīta DNS. Genotipēšana tika veikta…
Troksnis kā pacienta un personāla veselību ietekmējošs faktors intensīvās terapijas nodaļā
2015
Maģistratūras darba tēma – Troksnis kā pacientu un personāla veselību ietekmējošs faktors intensīvās terapijas nodaļā. Tēmas aktualitāti nosaka tas, ka troksnis ir nozīmīga vides problēma. Intensīvā terapija ir viena no medicīnas nozarēm, kurā strādājošajiem ir paaugstināta iespēja sastapties ar troksni kā darba vides riska faktoru. Darba mērķis ir aprakstīt un analizēt troksni kā faktoru, kas ietekmē pacienta un personāla veselību intensīvās terapijas nodaļā. Maģistra darbs sastāv no teorētiskās daļas un pētnieciskās daļas. Teorētiskajā daļā tika analizēti un apkopoti 50 literatūras avoti par troksni, trokšņa ietekmi uz fizioloģiju, psihiskām un sociālām problēmām, trokšņu īpatnībām, trokš…
Switching times in long-overlap Josephson junctions subject to thermal fluctuations and non-Gaussian noise sources
2014
We investigate the superconducting lifetime of long current-biased Josephson junctions, in the presence of Gaussian and non-Gaussian noise sources. In particular, we analyze the dynamics of a Josephson junction as a function of the noise signal intensity, for different values of the parameters of the system and external driving currents. We find that the mean lifetime of the superconductive state is characterized by nonmonotonic behavior as a function of noise intensity, driving frequency and junction length. We observe that these nonmonotonic behaviours are connected with the dynamics of the junction phase string during the switching towards the resistive state. An important role is played…
Integrating Decomposers, Methane-Cycling Microbes and Ecosystem Carbon Fluxes Along a Peatland Successional Gradient in a Land Uplift Region
2021
AbstractPeatlands are carbon dioxide (CO2) sinks that, in parallel, release methane (CH4). The peatland carbon (C) balance depends on the interplay of decomposer and CH4-cycling microbes, vegetation, and environmental conditions. These interactions are susceptible to the changes that occur along a successional gradient from vascular plant-dominated systems to Sphagnum moss-dominated systems. Changes similar to this succession are predicted to occur from climate change. Here, we investigated how microbial and plant communities are interlinked with each other and with ecosystem C cycling along a successional gradient on a boreal land uplift coast. The gradient ranged from shoreline to meadows…
The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser
2019
Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation of the protein through structural changes. We present serial femtosecond X-ray crystallographic data of the chromophore-binding domains of a bacterial phytochrome at delay times of 1 ps and 10 ps after photoexcitation. The data reveal a twist of the D-ring, which leads to partial detachment of the chromophore from the protein. Unexpectedly, the conserved so-called pyrrole water is photodissociated from the chromophore, concomitant with movement of the A-ring and a key signaling aspartate. The chan…
Apvērstā modeļa (Flipped classroom) principu izmantošana vācu valodas kā otrās svešvalodas nodarbībās vārdu krājuma apguvei un nostiprināšanai
2022
Diplomdarba tēma ir šāda: “Apvērstā modeļa (Flipped classroom) principu izmantošana vācu valodas kā otrās svešvalodas nodarbībās vārdu krājuma apguvei un nostiprināšanai". Diplomdarba mērķis ir izpētīt, kā apvērstā modeļa principus var izmantot vācu valodas nodarbībās pamatskolas 6. un 8. klasē vārdu krājuma bagātināšanai. Tiek pieņemts, ka, izmantojot apvērsto modeli klātienes mācībās un digitālos materiālus (video) patstāvīgai vārdu krājuma un gramatikas likumu atkārtošanai, izglītojamie var uzlabot savus mācību panākumus vārdu krājuma apguvē. Diplomdarba darba uzdevumi, kas kalpo mērķa sasniegšanai, ir šādi: teorētiskās literatūras un rakstu izpēte par attiecīgo tēmu, stundu plānu izstrā…