Search results for "label"
showing 10 items of 797 documents
Bimodal Effect of Amphiphilic Biocide Concentrations on Fluidity of Lipid Membranes
1996
Abstract Using the spin label method (ESR) it has been shown that biologically active, amphiphilic compounds (quaternary ammonium salts -AS) containing polar heads with single and double positive charge caused, at low concentrations, decrease fluidity of liposome membranes formed with egg yolk lecithin (EYL). At higher concentrations an increase in fluidity was observed. With compounds having a single positive charge minimum fluidity of membrane structure occurs in the range of 1 to 3%, with compounds containing double positive charge -in the range of 4 -6 % . That effect does not depend on polar head size and length of alkyl chains of the AS used. Analysis of the electrostatic interaction …
Biosilica-based immobilization strategy for label-free OWLS sensors
2013
Abstract In the last years, a new group of enzymes, so-called silicateins, have been identified and characterized, which form the axial filaments of the spicules of the siliceous sponges, consisting of amorphous silica. Silicateins are able to catalyze the polycondensation and deposition of silica at mild conditions (low temperature and physiological pH). By means of these enzymes it is possible for the first time to produce silica nanostructures biocatalytically, which opens new ways for construction of biosensors. The cDNAs encoding the responsible enzymes have been isolated and the proteins can be produced in a recombinant way. Here we demonstrate the silicatein-mediated biosilica format…
Photoaffinity labeling of the coupling factor 1 from the thermophilic bacterum PS3 by 8-azido ATP
1984
AbstractTo localize the nucleotide binding sites of the F1ATPase (TF1) from the thermophilic bacterium PS3 we have used 14C-labeled 8-azido ATP (8-N3ATP) as photoaffmity label. 8-N3ATP is hydrolyzed by the F,ATPase in the absence of ultraviolet light. Irradiation by ultraviolet light of the enzyme in the presence of 8-N3ATP results in reduction of ATPase activity and in preferential nucleotide specific labeling of the α subunits (0.8–0.9 mol 8-N3ATP/TF1,α:β = 4:1). Inactivation and labeling do not depend on the presence of Mg2+. Both effects decrease upon addition of various nucleotide di- or triphosphates.
Radiolabeling of DOTATOC with the long-lived positron emitter 44Sc.
2010
Abstract The positron-emitting radionuclide 44 Sc with a half-life of 3.97 h and a β + branching of 94.3% is of potential interest for clinical PET. As so far it is available from a 44 Ti/ 44 Sc generator in Mainz, where long-lived 44 Ti decays to no-carrier-added (nca) 44 Sc. The 44 Sc is a trivalent metal cation and should be suitable for complexation with many well established bifunctional chelators conjugated to peptides or other molecular targeting vectors. Thus, the aim of this work was to investigate the potential of 44 Sc for labeling of DOTA-conjugated peptides. DOTA-D-Phe 1 -Tyr 3 -octreotide (DOTATOC) was used as a model molecule to study and optimize labeling procedure. Reaction…
Efficient alkali iodide promoted 18F-fluoroethylations with 2-[18F]fluoroethyl tosylate and 1-bromo-2-[18F]fluoroethane
2003
Abstract Radiochemical 18 F-fluorination yields of several compounds using the secondary labelling precursors 2-[ 18 F]fluoroethyl tosylate ([ 18 F]FETos) and 1-bromo-2-[ 18 F]fluoroethane ([ 18 F]BFE) could be considerably enhanced by the addition of an alkali iodide. The radiochemical yield of [ 18 F]fluoroethyl choline for example could be doubled with [ 18 F]BFE and increased from 13% to ≈80% with [ 18 F]FETos. By addition of alkali iodide to the precursor, the 18 F-fluoroethylation yields of established radiopharmaceuticals, especially in the case of automated syntheses, could be significantly increased without major changes of the reaction conditions.
In vivo analysis of noise dependent activation of white blood cells and microvascular dysfunction in mice
2021
This article contains supporting information on data collection for the research article entitled “Aircraft noise exposure drives the activation of white blood cells and induces microvascular dysfunction in mice” by Eckrich et al. We found that noise-induced stress triggered microvascular dysfunction via involvement of innate immune-derived reactive oxygen species. In this article, we present the instrumentation of mice with dorsal skinfold chambers for in vivo microscopic imaging of blood flow, interaction of leukocytes with the vascular wall (also by fluorescent labelling of blood cells) and vessel diameter. In addition, we explain the preparation of cerebral arterioles for measurement of…
Is “I-DNA” derived from Nuclear DNA ?
1970
On the basis of double radioactive labelling and buoyant density studies, it is concluded that “I-DNA” is not a separate entity from nuclear DNA but may be an artefact derived from it.
Climate Change Mitigation Potentials of Carbon Labels in the Aviation Industry
2018
This chapter discusses the climate change mitigation potentials of carbon labels in the aviation industry. As this study shows, there are significant differences in the environmental performance of individual flights, even when operated on the same route or by the same airline. Selecting one flight over another can significantly reduce the carbon dioxide emissions of a single air passenger. Nevertheless, the currently available measures, such as carbon calculators, are not capable of presenting these differences, leaving air passengers with no means of comparing individual flights. Therefore, the use of carbon labels could be highly beneficial because it would give air passengers the opport…
Does ns2 Really Simulate MPLS Networks?
2008
Multi Protocol Label Switching (MPLS) is being used in many corporate networks and public infrastructures and as a backbone technology of many Autonomous Systems. Because of its importance, what is needed is to find out simulators able to simulate MPLS networks whose results reflect the real environment as much as possible. In this article, we will show real measurements from the Railway Infrastructure Administrator (ADIF, the Spanish railway infrastructure manager) MPLS network and we will compare them with the results obtained by the freeware simulator ns2. We will check the level of reliability provided by the simulator and we will know under which parameters its results will be similar …
Qualité et coordination économique dans les industries agroalimentaires. Analyse institutionnelle comparée de l'industrie du vin en Bourgogne, Califo…
2004
Since the 1990s, the French wine exporters have been confronted with an increasing competition from a few nations of North America and the Southern Hemisphere. Initially positioned on the medium-range, the New World Wine Producers quickly developed their positions on the whole of the segments, including those traditionally occupied by French wines under controlled label of origin (AOC). The thesis examines the link between wine marketer' quality strategies and the coordination in the chain of production. Research is carried out on two levels: that of transactions governance structures concerning fresh grape, must and bulk wine, and that of the public rules framing these transactions. Firstl…