Search results for "lasi"

Infliximab and newly diagnosed neoplasia in CD:a multicenter matched pair study

2006

Background and aims: The widespread use of anti-tumour necrosis factor α antibody (Infliximab) in Crohn's disease (CD) raises concerns about a possible cancer risk in the long term. In a matched pair study, we assessed whether Infliximab is associated with an increased risk of neoplasia. Methods: In a multicentre matched pair study, 404 CD patients treated with Infliximab (CD-IFX) were matched with 404 CD patients who had never received Infliximab (CD-C). Cases and controls were matched for sex, age (±5 years), site of CD, age at diagnosis (±5 years), immunosuppressant use, and follow up. New diagnoses of neoplasia from April 1999 to October 2004 were recorded. Results: Among the 404 CD-IFX…

Vitrification of Immature Porcine Oocytes: Effects of Lipid Droplets, Temperature, Cytoskeleton, and Addition and Removal of Cryoprotectant

1998

Three experiments were conducted to investigate the effects of single-step and stepwise exposure to and removal of cryoprotectant, of temperature, and of a cytoskeletal relaxant on the development of germinal vesicle porcine oocytes to the M-II stage. In experiment I, noncooled cumulus-oocyte complexes (COCs) were treated using single-step/stepwise exposure to ethylene glycol (EG) and removal at 23 or 42 degrees C. Stepwise exposure to EG and dilution at 42 degrees C were found to have a positive effect on the COC developmental rate. In experiment II, also without cooling, COCs were treated with Cytochalasin B at 42 degrees C using single-step and stepwise protocols of exposure to and remov…

Pasajes de París / 4: Sus usos culturales

2006

Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.

2013

Respiratory syncytial virus (RSV) causes acute exacerbations in COPD and asthma. RSV infects bronchial epithelial cells (HBE) that trigger RSV associated lung pathology. This study explores whether the phosphodiesterase 4 (PDE4) inhibitor Roflumilast N-oxide (RNO), alters RSV infection of well-differentiated HBE (WD-HBE) in vitro. WD-HBE were RSV infected in the presence or absence of RNO (0.1-100 nM). Viral infection (staining of F and G proteins, nucleoprotein RNA level), mRNA of ICAM-1, ciliated cell markers (digital high speed videomicroscopy, β-tubulin immunofluorescence, Foxj1 and Dnai2 mRNA), Goblet cells (PAS), mRNA of MUC5AC and CLCA1, mRNA and protein level of IL-13, IL-6, IL-8, T…

Loss of surface fibronectin after infection of cultured cells by HSV-1 and 2

1985

Fibronectin is lost from the surface of HSV infected cells during cell rounding. In order to investigate also the fate of fibronectin during the process of HSV-induced cell-fusion, BHK, Vero as well as primary or secondary rabbit kidney cells were infected with HSV-1 strains producing cell-fusion. By immunofluorescence and immunoelectron microscopy a considerable loss of fibronectin after HSV infection could be demonstrated leaving only irregular clumps of fibronectin containing virus particles on the cell surface. Decrease and disarrangement of fibronectin was similar during cell rounding and cell fusion. Loss of Fibronectin was closely connected with the two types of the cytopathic effect…

Implicaciones ópticas y biomecánicas de la instilación de anestesia tópica sobre ojos normales y con queratomileusis intraestromal con láser (LASIK)

2013

La córnea es el elemento refractivo más potente del sistema ocular, ya que proporciona las dos terceras partes del poder dióptrico total del ojo y condiciona en gran medida la calidad de la imagen retiniana. Esta relevancia que presenta la córnea en el proceso de la visión, justifica su caracterización pormenorizada en la consulta oftalmológica, así como su elección como tejido diana en multitud de técnicas quirúrgicas encaminadas a proporcionar la emetropía, técnicas entre las que destaca por su popularidad la queratomileusis intraestromal con láser excímer (LASIK). Dada la densa y compleja inervación que presenta, la instilación de anestesia tópica sobre la superficie ocular resulta una p…

Detection of cytokeratin dynamics by time-lapse fluorescence microscopy in living cells.

1999

To monitor the desmosome-anchored cytokeratin network in living cells fusion protein HK13-EGFP consisting of human cytokeratin 13 and the enhanced green fluorescent protein was stably expressed in vulvar carcinoma-derived A-431 cells. It is shown for A-431 subclone AK13-1 that HK13-EGFP emits strong fluorescence in fixed and living cells, being part of an extended cytoplasmic intermediate filament network that is indistinguishable from that of parent A-431 cells. Biochemical, immunological and ultrastructural analyses demonstrate that HK13-EGFP behaves identically to the endogenous cytokeratin 13 and is therefore a reliable in vivo tag for this polypeptide and the structures formed by it. T…

Oratory and Classicism: a Valencian speech of the 16th century in its classical sources

2006

Este artículo estudia un discurso pronunciado solemnemente en el acto de apertura del curso académico 1531-1532 en la Universidad de Valencia a partir del examen de sus fuentes clásicas y del análisis de sus recursos retóricos. Se trata de la Oratio parenetica de óptimo statu reipublicae constituendo, el único de los escritos que sepamos que se ha conservado del oriolano Cosme Damián Çavall, primer catedrático de griego del Estudi General de la ciudad, discípulo de Elio Antonio de Nebrija y Juan Andrés Strany y maestro de Miguel Jerónimo Ledesma. This article studies a solemn discourse pronounced at the University of Valencia in 1531 after its classical sources and after its rhetorical reso…

Biases in study design, implementation, and data analysis that distort the appraisal of clinical benefit and ESMO-Magnitude of Clinical Benefit Scale…

2021

BACKGROUND: The European Society for Medical Oncology-Magnitude of Clinical Benefit Scale (ESMO-MCBS) is a validated, widely used tool developed to score the clinical benefit from cancer medicines reported in clinical trials. ESMO-MCBS scores assume valid research methodologies and quality trial implementation. Studies incorporating flawed design, implementation, or data analysis may generate outcomes that exaggerate true benefit and are not generalisable. Failure to either indicate or penalise studies with bias undermines the intention and diminishes the integrity of ESMO-MCBS scores. This review aimed to evaluate the adequacy of the ESMO-MCBS to address bias generated by flawed design, im…

YOLO pieeja cilvēku kustību klasificēšanai

2021

Cilvēku aktivitātes klasificēšana ar viedierīču palīdzību ir noderīga gan medicīnas pielietojumiem, gan sporta pielietojumiem, un citiem. Piemēram, pateicoties tai ir iespējams atpazīt lietotāja kritienu un savlaicīgi izsaukt palīdzību. Šajā darbā ir apskatīts kā tiek veikta cilvēku kustību atpazīšana un sportisko aktivitāšu atkārtojumu skaitīšana ar viedtālruņiem. Darba praktiskajā daļā ir ievākti kustību dati no vairākiem cilvēkiem veicot pietupienus. Šie dati izmantoti, lai izveidotu dziļo neironu tīklu modeli, kurš spēj atpazīt pietupienus, kā arī saskaitīt to atkārtojumu skaitu. Darbā tiek piedāvāts izmantot konvolūciju līmeņus un idejas no YOLO dziļo neironu tīkla, kuru izmanto objekt…