Search results for "layer"

Layer-by-layer assembled protein/polymer hybrid films: nanoconstruction via specific recognition

1998

Abstract In the present study it is shown that streptavidin-containing multilayer films with varying numbers of polyelectrolyte spacer layers can be fabricated reproducibly using optimized deposition conditions. Direct alternation of streptavidin and PLB leads to multilayer systems with an average streptavidin thickness of 5.3 nm which is in good agreement with the dimensions of the protein. When the streptavidin layers are spacered by more polyelectrolyte layers the distance between the protein sheets is increased up to e.g. 6.5 nm in the case of (PLB/PSS/PAH/PSS/PLB) as spacer layer. X-ray reflectivity reveals that streptavidin increases the surface roughness of the films probably due to …

Bilayer Formation of Streptavidin Bridged by Bis(biotinyl) Peptide at the Air/Water Interface

1994

Specific Protein Binding to Functionalized Interfaces

1992

We report on the characterization of specific binding reactions between streptavidin and biotinylated model membrane surfaces. Self-assembly techniques as well as the Langmuir-Blodgett-Kuhn method were employed to prepare reactive, functionalized surfaces on various solid supports in contact with the aqueous protein solution. Plasmon surface polaritons optical measurements as well as atomic force microscopy and studies with the surface forces apparatus give rather detailed information as to the streptavidin monolayer formation, the kinetics of this process (either binding site- or diffusion limited), the selectivity of the reaction at laterally heterogeneous membranes, and the involved inte…

Protein-Membrane Interaction

2012

In the following chapter I present a nanosized and addressable protein sensor platform based on membrane coated plasmonic particles. I show unequivocally the covering of gold nanorods with lipid bilayers and thereafter the subsequent detection of streptavidin binding to biotin moities in the lipids. The binding of the streptavidin on the membrane covered gold nanorods is detected by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel.

Surface effects, boundary conditions and evolution laws within second strain gradient plasticity

2014

Abstract The principle of the virtual power (PVP) is used in conjunction with the concepts of “energy residual” and “insulation condition” to address second strain gradient plasticity. The energy residual with its typical divergence format is an extra stress power playing the role of basic state variable to describe the gradient effects, whereas the insulation condition constitutes a global energy characterization of the body as part of the body/environment system. The microstructure of a second strain gradient material (but not of a first strain gradient one) is shown to exhibit surface effects with the formation of a thin boundary layer. This boundary layer is in local (and global) equili…

A unifying variational framework for stress gradient and strain gradient elasticity theories

2015

Abstract Stress gradient elasticity and strain gradient elasticity do constitute distinct continuum theories exhibiting mutual complementary features. This is probed by a few variational principles herein presented and discussed, which include: i) For stress gradient elasticity, a (novel) principle of minimum complementary energy and an (improved-form) principle of stationarity of the Hellinger–Reissner type; ii) For strain gradient elasticity, a (known) principle of minimum total potential energy and a (novel) principle of stationarity of the Hu–Washizu type. Additionally, the higher order boundary conditions for stress gradient elasticity, previously derived by the author (Polizzotto, Int…

Electron-hole bilayer quantum dots: Phase diagram and exciton localization

2003

We studied a vertical ``quantum dot molecule'', where one of the dots is occupied with electrons and the other with holes. We find that different phases occur in the ground state, depending on the carrier density and the interdot distance. When the system is dominated by shell structure, orbital degeneracies can be removed either by Hund's rule, or by Jahn-Teller deformation. Both mechanisms can lead to a maximum of the addition energy at mid-shell. At low densities and large interdot distances, bound electron-hole pairs are formed.

Crystallization of SrCO3 on a self-assembled monolayer substrate: an in-situ synchrotron X-ray study

2001

Self-assembled monolayers (SAMs) of alkanethiols on gold surfaces show great promise in controlling the nucleation and growth of inorganic minerals from solution. In doing so, they mimic the role of some biogenic macromolecules in natural biomineralisation processes. Crystallization on SAM surfaces is usually monitored ex-situ; by allowing the process to commence and to evolve for some time, removing the substrate from the mother solution, and then examining it using microscopy, diffraction etc. We present here for the first time, the use of high energy monochromatic synchrotron X-radiation in conjunction with a two dimensional detector to monitor in situ, in a time resolved fashion, the gr…

Templated Crystallisation of Calcium and Strontium Carbonates on Centred Rectangular Self-Assembled Monolayer Substrates

1998

SrCO3crystals display patterns of templating when grown on tailored self-assembled monolayers (right). As SrCO3 is isostructural with aragonite, comparison of the crystallisation of SrCO3 in the aragonite/strontianite modification with the crystallisation of CaCO3 in all three modifications might yield some insights into which factors are important for crystal growth.

Artificial neural network comparison for a SHM procedure applied to composite structures.

2013

In this paper different architectures of Artificial Neural Networks (ANNs) for structural damage detection are studied. The main objective is to create an ANN able to detect and localize damage without any prior knowledge on its characteristics so as to serve as a realtime data processor for SHM systems. Two different architectures are studied: the standard feed-forward Multi Layer Perceptron (MLP) and the Radial Basis Function (RBF) ANNs. The training data are given, in terms of a Damage Index ℑD, properly defined using the piezoelectric sensor signal output to obtain suitable information on the damage position and dimensions. The electromechanical response of the assembled structure has b…