Search results for "lcsh:M"

showing 10 items of 4818 documents

Oxygen Availability during Growth Modulates the Phytochemical Profile and the Chemo-Protective Properties of Spinach Juice.

2018

Fruits and vegetables are a good source of potentially biologically active compounds. Their regular consumption in the human diet can help reduce the risk of developing chronic diseases such as cardiovascular diseases and cancer. Plants produce additional chemical substances when subject to abiotic stress or infected by microorganisms. The phytochemical profile of spinach leaves (Spinacia oleracea L.), which is a vegetable with widely recognized health-promoting activity, has been affected by applying root hypoxic and re-oxygenation stress during plant growth. Leaf juice at different sampling times has been subject to liquid chromatography mass spectrometry (LC-MSn) analysis and tested on t…

0106 biological sciences0301 basic medicineSpinaciaAntioxidantHT29 cell lineCell Survivalmedicine.medical_treatmentLiquid Chromatography-Mass Spectrometry<i>Spinacia oleracea</i> L.lcsh:QR1-502antioxidant activitySpinacia oleracea L.Anti-proliferative activity; Antioxidant activity; Comet Assay; HT29 cell line; Liquid Chromatography-Mass Spectrometry; Spinacia oleracea L;medicine.disease_cause01 natural sciencesBiochemistrylcsh:MicrobiologyAntioxidantsMass SpectrometryArticle03 medical and health sciencesSpinacia oleraceamedicineHumansFood scienceMolecular BiologyCell ProliferationbiologyAbiotic stressChemistryChemistry PhysicalPlant Extractsfood and beveragesBiological activitybiology.organism_classificationAntineoplastic Agents PhytogenicComet assayFruit and Vegetable JuicesOxygen030104 developmental biologyPhytochemicalSpinachanti-proliferative activityComet AssayDrug Screening Assays AntitumorHT29 CellsOxidative stress010606 plant biology & botanyChromatography LiquidBiomolecules
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Molecular Responses to Small Regulating Molecules against Huanglongbing Disease

2016

Huanglongbing (HLB; citrus greening) is the most devastating disease of citrus worldwide. No cure is yet available for this disease and infected trees generally decline after several months. Disease management depends on early detection of symptoms and chemical control of insect vectors. In this work, different combinations of organic compounds were tested for the ability to modulate citrus molecular responses to HLB disease beneficially. Three small-molecule regulating compounds were tested: 1) L-arginine, 2) 6-benzyl-adenine combined with gibberellins, and 3) sucrose combined with atrazine. Each treatment contained K-phite mineral solution and was tested at two different concentrations. T…

0106 biological sciences0301 basic medicineSucroseLeavesCitruslcsh:MedicineGene ExpressionSecondary MetabolismPlant ScienceDisaccharidesBiochemistry01 natural sciencesStarchesGene Expression Regulation PlantINFECTIONMedicine and Health SciencesInnatePlant HormonesAmino Acidslcsh:ScienceImmune ResponseGENE-EXPRESSIONMultidisciplinaryNONHOST RESISTANCEbiologyOrganic CompoundsPlant BiochemistryPlant AnatomyChemistryPhenotypeBiochemistryDEFENSE RESPONSESCANDIDATUS-LIBERIBACTER-ASIATICUS; ARABIDOPSIS-THALIANA; NONHOST RESISTANCE; DEFENSE RESPONSES; CITRUS-SINENSIS; GENE-EXPRESSION; INFECTION; PLANTS; IDENTIFICATION; TRANSCRIPTOMEPhysical SciencesHost-Pathogen InteractionsCarbohydrate MetabolismSucrose synthaseAtrazineGibberellinBasic Amino AcidsStarch synthaseSystemic acquired resistanceResearch ArticleCITRUS-SINENSISGeneral Science & TechnologyPhysiologicalImmunologyCarbohydratesCarbohydrate metabolismStressArginine03 medical and health sciencesStress PhysiologicalSettore AGR/07 - Genetica AgrariaGeneticsPLANTSTRANSCRIPTOMESecondary metabolismGenePlant DiseasesIDENTIFICATIONGene Expression Profilinglcsh:ROrganic ChemistryImmunityChemical CompoundsBiology and Life SciencesProteinsPlantBiotic stressCANDIDATUS-LIBERIBACTER-ASIATICUSHormonesGibberellinsImmunity InnateMetabolism030104 developmental biologyGene Expression RegulationARABIDOPSIS-THALIANAbiology.proteinlcsh:Q010606 plant biology & botanyPLOS ONE
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Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cr…

2018

The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Cre…

0106 biological sciences0301 basic medicineSympatryHereditySpeciationlcsh:MedicineDisease Vectors01 natural sciencesBiochemistryGeographical LocationsHyalestheslcsh:SciencePhylogenyEnergy-Producing OrganellesMultidisciplinarybiologyGeographyEcologyCixiidaeMitochondrial DNAMitochondriaEuropeNucleic acidsSympatryGenetic MappingPhylogeographyBiogeographySympatric speciationHost-Pathogen InteractionsCellular Structures and OrganellesResearch ArticleGenetic MarkersSpecies complexPhytoplasmaEvolutionary ProcessesEcological MetricsForms of DNAZoologyBioenergetics010603 evolutionary biologyHemiptera03 medical and health sciencesPlanthopperGenetic variationCryptic SpeciationGeneticsAnimalsPlant DiseasesEvolutionary BiologyPopulation Biologylcsh:REcology and Environmental SciencesBiology and Life SciencesSpecies DiversityDNACell Biology15. Life on landbiology.organism_classificationInsect Vectors030104 developmental biologyHaplotypesGenetic markerGenome MitochondrialPeople and PlacesEarth Scienceslcsh:QPopulation Genetics
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Need for speed : short lifespan selects for increased learning ability

2019

AbstractIt is generally assumed that an investment into cognitive abilities and their associated cost is particularly beneficial for long-lived species, as a prolonged lifespan allows to recoup the initial investment. However, ephemeral organisms possess astonishing cognitive abilities too. Invertebrates, for example, are capable of simple associative learning, reversal learning, and planning. How can this discrepancy between theory and evidence be explained? Using a simulation, we show that short lives can actually select for an increase in learning abilities. The rationale behind this is that when learning is needed to exploit otherwise inaccessible resources, one needs to learn fast in o…

0106 biological sciences0301 basic medicineTime FactorsComputer scienceBehavioural ecologyEvolutionLongevityanimal behaviourevoluutiolcsh:MedicineReversal Learning010603 evolutionary biology01 natural sciencesModels Biologicaleläinten käyttäytyminenArticle03 medical and health sciencesCognitionOrder (exchange)evolutionAnimalsComputer Simulationlcsh:ScienceMultidisciplinaryBehavior Animallcsh:RBrainbehavioural ecologyCognitionAnimal behaviourInvestment (macroeconomics)Biological EvolutionekologiaAssociative learning030104 developmental biologylcsh:QCognitive psychology
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The In Vitro Interaction of 12-Oxophytodienoic Acid and Related Conjugated Carbonyl Compounds with Thiol Antioxidants

2021

α,β-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols of metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) and abscisic acid (ABA) conjugated with cysteine. It was found that the reactivity of α,β-unsaturated carbonyls with GSH followed the sequence trans-2-hexenal &lt

0106 biological sciences0301 basic medicinecysteine covalent modification570Isomerase activityArabidopsis thalianaArabidopsislcsh:QR1-50201 natural sciencesBiochemistryArticleAntioxidantslcsh:Microbiology03 medical and health scienceschemistry.chemical_compoundThioredoxinsPlant Growth RegulatorsmedicineCysteineSulfhydryl CompoundsMolecular BiologyCyclophilinchemistry.chemical_classificationChemistry<i>Arabidopsis thaliana</i>peroxiredoxinGlutathionethioredoxinphytohormones030104 developmental biologyMechanism of actionBiochemistryprotein–ligand interactioncyclophilinThiolFatty Acids Unsaturatedmedicine.symptomThioredoxinPeroxiredoxinthiol antioxidants010606 plant biology & botanyCysteineBiomolecules
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Variation in gene expression within clones of the earthworm Dendrobaena octaedra

2017

Gene expression is highly plastic, which can help organisms to both acclimate and adapt to changing environments. Possible variation in gene expression among individuals with the same genotype (among clones) is not widely considered, even though it could impact the results of studies that focus on gene expression phenotypes, for example studies using clonal lines. We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. Five microsatellite markers were developed and used to confirm that offspring are genetic clones of their parent. After that, expression of 12 genes was measured…

0106 biological sciences0301 basic medicinegenotypeClone (cell biology)lcsh:Medicineearthworms01 natural sciencesMicrosatellite LociDendrobaena octaedraGenotypeGene expressiongeeniekspressioAnnelidslcsh:ScienceGeneticsMultidisciplinarykloonitPhenotypePhenotypesMicrosatelliteGene CloningResearch ArticleGenotypingBiologyResearch and Analysis Methods010603 evolutionary biologygenotyyppi03 medical and health sciencesExtraction techniquesclonesGene TypesApomixisGeneticsAnimalsOligochaetaparthenogenesisMolecular Biology TechniquesMolecular BiologyGenepartenogeneesita1184lcsh:ROrganismsBiology and Life SciencesParthenogenesisInvertebratesRNA extraction030104 developmental biologygene expressionta1181lcsh:QMicrosatellite RepeatsCloningPLoS ONE
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Random Genetic Drift and Selective Pressures Shaping the Blattabacterium Genome

2018

AbstractEstimates suggest that at least half of all extant insect genera harbor obligate bacterial mutualists. Whereas an endosymbiotic relationship imparts many benefits upon host and symbiont alike, the intracellular lifestyle has profound effects on the bacterial genome. The obligate endosymbiont genome is a product of opposing forces: genes important to host survival are maintained through physiological constraint, contrasted by the fixation of deleterious mutations and genome erosion through random genetic drift. The obligate cockroach endosymbiont, Blattabacterium – providing nutritional augmentation to its host in the form of amino acid synthesis – displays radical genome alterations…

0106 biological sciences0301 basic medicinelcsh:MedicineCockroachesBacterial genome size010603 evolutionary biology01 natural sciencesGenomeArticleEvolution Molecular03 medical and health sciencesBlattabacteriumGenetic driftAnimalslcsh:ScienceSymbiosisGeneMultidisciplinaryObligatebiologyHost (biology)lcsh:RGenetic Driftfungibiology.organism_classificationFixation (population genetics)030104 developmental biologyEvolutionary biologylcsh:QFlavobacteriaceaeGenome BacterialScientific Reports
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Foraging behaviour of an egg parasitoid exploiting plant volatiles induced by pentatomids : The role of adaxial and abaxial leaf surfaces

2017

Several phases of herbivorous insect attack including feeding and oviposition are known to induce plant defenses. Plants emit volatiles induced by herbivores to recruit insect parasitoids as an indirect defense strategy. So far, volatiles induced by herbivore walking and their putative role in the foraging behavior of egg parasitoids have not been investigated. In this paper we studied the response of the egg parasitoid Trissolcus basalis toward volatiles emitted by Vicia faba plants as consequence of the walking activity of the host Nezara viridula. Olfactometer bioassays were carried out to evaluate wasp responses to plants in which the abaxial or the adaxial surfaces were subjected to wa…

0106 biological sciences0301 basic medicinemedia_common.quotation_subjectLeaf surfaceOvipositionlcsh:MedicineInsectPlant ScienceChemical ecology01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyParasitoid03 medical and health sciencesBotanyPlant defense against herbivoryWalking activityLaboratory of EntomologyTrissolcus basalisChemical ecology; Leaf surface; Nezara viridula; Oviposition; Trissolcus basalis; Walking activity; Neuroscience (all); Medicine (all); Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)media_commonHerbivoreNeuroscience (all)Biochemistry Genetics and Molecular Biology (all)biologyHost (biology)Medicine (all)General NeuroscienceChemical ecology; Leaf surface; Nezara viridula; Oviposition; Trissolcus basalis; Walking activitylcsh:RfungiNezara viridulafood and beveragesTrissolcus basaliGeneral Medicinebiology.organism_classificationLaboratorium voor EntomologieChemical ecology010602 entomologySettore AGR/11 - Entomologia Generale E Applicata030104 developmental biologyAgricultural and Biological Sciences (all)OlfactometerNezara viridulaEPSGeneral Agricultural and Biological SciencesEntomology017-4020PeerJ
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Phytotoxic Metabolites Isolated from Neufusicoccum batangarum, the Causal Agent of the Scabby Canker of Cactus Pear (Opuntia ficus-indica L.)

2020

Six phytotoxins were obtained from the culture filtrates of the ascomycete Neofusicoccum batangarum, the causal agent of the scabby canker of cactus pear (Opuntia ficus-indica L.) in minor Sicily islands. The phytotoxins were identified as (&minus

0106 biological sciences<i>neofusicoccum batangarum</i>Health Toxicology and MutagenesisOpuntia ficuslcsh:MedicineBiologyToxicology01 natural sciencesArticlephytotoxinsAscomycotamedicineNuclear Magnetic Resonance BiomolecularPlant DiseasesCankerPEARphytotoxinMolecular Structure010405 organic chemistryHost (biology)lcsh:ROpuntiaNeofusicoccum batangarumMycotoxinsmedicine.diseaseNeofusicoccum batangarum0104 chemical sciencesHorticultureFruitCactuscactus pearPhytotoxicityscabby cankers010606 plant biology & botanyToxins
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