Search results for "lino"
showing 10 items of 1310 documents
Pyrrolo[2,3-h]quinolinones: A new ring system with potent photoantiproliferative activity
2006
A new class of compounds, the pyrrolo[2,3-h]quinolin-2-ones, nitrogen isosters of the angular furocoumarin Angelicin, was synthesized with the aim of obtaining new photochemotherapeutic agents with increased antiproliferative activity and lower undesired toxic effects than the lead compound. Two synthetic pathways were approached to allow the isolation both of the dihydroderivatives 10-17 and of the aromatic ring system 23. Compounds 10-17 showed a remarkable phototoxicity and a great UVA dose dependence reaching IC(50) values at submicromolar level. Intracellular localization of these compounds has been evaluated by means of fluorescence microscopy using tetramethylrhodamine methyl ester a…
Thermal Induction of 9t12t Linoleic Acid: a New Pathway for the Formation of Conjugated Linoleic Acids
2009
Accepted version of an article published in the journal: International Journal of Engineering and Technology Published version available at: http://www.ijetch.org/papers/066.pdf Thermal induction of 9t12t fatty acid leading to the formation of Conjugated Linoleic Acids (CLA) has been demonstrated by subjecting glyceride, and methyl ester of the acid to heat treatment. Fifteen micro liter portions of the glyceride sample containing 9t12t fatty acid (trilinoelaidin) were placed in micro glass ampoules and sealed under nitrogen and then subjected to thermal treatment at 250oC. The glass ampoules were taken out at regular time intervals, and the contents were subjected to composition analysis b…
Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis
2011
Fibronectin (FN) is a major component of the extracellular matrix and functions in cell adhesion, cell spreading and cell migration. In the retina, FN is transiently expressed and assembled on astrocytes (ACs), which guide sprouting tip cells and deposit a provisional matrix for sprouting angiogenesis. The precise function of FN in retinal angiogenesis is largely unknown. Using genetic tools, we show that astrocytes are the major source of cellular FN during angiogenesis in the mouse retina. Deletion of astrocytic FN reduces radial endothelial migration during vascular plexus formation in a gene dose-dependent manner. This effect correlates with reduced VEGF receptor 2 and PI3K/AKT signalli…
Let it flow: Morpholino knockdown in zebrafish embryos reveals a pro-angiogenic effect of the metalloprotease meprin alpha2.
2010
BACKGROUND: Meprin metalloproteases are thought to be involved in basic physiological functions such as cell proliferation and tissue differentiation. However, the specific functions of these enzymes are still ambiguous, although a variety of growth factors and structural proteins have been identified as meprin substrates. The discovery of meprins alpha(1), alpha(2) and beta in teleost fish provided the basis for uncovering their physiological functions by gene silencing in vivo. METHODOLOGY/PRINCIPAL FINDINGS: A Morpholino knockdown in zebrafish embryos targeting meprin alpha(1) and beta mRNA caused defects in general tissue differentiation. But meprin alpha(2) morphants were affected more…
Su alcune piante nuove delle Isole Pelagie
2013
On some new plants to Pelagic islands (Straits of Sicily) – Fifteen vascular plants belonging to the families Aizoaceae, Amaryllidaceae, Asteraceae, Brassicaceae, Euphorbiaceae, Myoporaceae, Orobanchaceae, Oxalidaceae, Portulacaceae and Solanaceae are reported as new to one or both the major Pelagic islands. With the exception of Narcissus serotinus and Orobanche minor, they are non-native plants, probably of recent human introduction, mostly in areas heavily modified by man. Myoporum insulare, widely used for reforestation and for ornament, occurs also in sub and semi-natural environments.
Phosphatidylinositol phosphate kinase type I gamma regulates dynamics of large dense-core vesicle fusion.
2005
Phosphatidylinositol-4,5-bisphosphate was proposed to be an important regulator of large dense-core vesicle exocytosis from neuroendocrine tissues. Here, we have examined the kinetics of secretion in chromaffin cells from mice lacking phosphatidylinositol phosphate kinase type Iγ, the major neuronal phosphatidylinositol-4-phosphate 5-kinase. Absence of this enzyme caused a reduction of the readily releasable vesicle pool and its refilling rate, with a small increase in morphologically docked vesicles, indicating a defect in vesicle priming. Furthermore, amperometry revealed a delay in fusion pore expansion. These results provide direct genetic evidence for a key role of phosphatidylinositol…
Calcium-dependent conformational changes of membrane-bound Ebola fusion peptide drive vesicle fusion
2003
AbstractThe fusogenic subdomain of the Ebola virus envelope glycoprotein is an internal sequence located ca. 20 residues downstream the N-terminus of the glycoprotein transmembrane subunit. Partitioning of the Ebola fusion peptide into membranes containing phosphatidylinositol in the absence of Ca2+ stabilizes an α-helical conformation, and gives rise to vesicle efflux but not vesicle fusion. In the presence of millimolar Ca2+ the membrane-bound peptide adopts an extended β-structure, and induces inter-vesicle mixing of lipids. The peptide conformational polymorphism may be related to the flexibility of the virus–cell intermembrane fusogenic complex.
Differential involvement of mussel hemocyte sub-populations in the clearance of bacteria.
2008
Abstract Mussels are filter-feeders living in a bacteria-rich environment. We have previously found that numerous bacterial species are naturally present within the cell-free hemolymph, including several of the Vibrio genus, whereas the intra-cellular content of hemocytes was sterile. When bacteria were injected into the circulation of the mussel, the number of living intra-hemocyte bacteria dramatically increased in less than an hour, suggesting intense phagocytosis, then gradually decreased, with no viable bacteria remaining 12 h post-injection for Micrococcus lysodeikticus, 24 h for Vibrio splendidus and more than 48 h for V ibrio anguillarum. The total hemocyte count (THC) was dramatica…
Comparison of three activity monitors for estimating sedentary time among children
2016
Background: Time spent sedentary appears to be associated with several health outcomes in adults, but findings are inconsistent in children. Further, the assessment of sedentary time represents a major challenge. The objectives of the present study were to determine whether 1) ActiGraph GT3X+, ActivPAL and SenseWear Armband Pro3 (SWA) provide comparable estimates of sedentary time in 9–12-year-old children, 2) these devices are valid compared with direct observation, and 3) ActivPAL discriminates between sitting and standing behavior. Methods: The sample was 67 children. Data were collected during three consecutive days in November 2012. To test the activity monitors in contexts related to …
Villino Basile a Palermo. La casa-studio come opera manifesto per una variabile latna della nuova cultura dell'abitare modernista
2016
Ricordata, ancora all’inizio degli anni Ottanta del XX secolo, da Giuseppe Spatrisano come “architettura dalla modernità esemplare” che negli anni Venti si imponeva ancora per la sua semplice raffinatezza, in una scena urbana oramai affetta da una variegata patologia tradizionalista, la casa-studio palermitana di Ernesto Basile non poteva però assumere in toto il ruolo di modello declinabile per l’architettura modernista siciliana. Detta anche “villino Ida” in omaggio alla consorte, la casa di Ernesto Basile, ad onta della volumetria discreta e della calligrafica strumentazione formale dal nitido tenore astilo, si distingueva rispetto alla pur elegante compagine edilizia circostante, costru…