Search results for "lost"

showing 10 items of 626 documents

Microbial communities of polluted sub-surface marine sediments

2018

Abstract Microbial communities of coastal marine sediment play a key role in degradation of petroleum contaminants. Here the bacterial and archaeal communities of sub-surface sediments (5–10 cm) of the chronically polluted Priolo Bay (eastern coast of Sicily, Italy), contaminated mainly by n-alkanes and biodegraded/weathered oils, were characterized by cultural and molecular approaches. 16S-PCR-DGGE analysis at six stations, revealed that bacterial communities are highly divergent and display lower phylogenetic diversity than the surface sediment; sub-surface communities respond to oil supplementation in microcosms with a significant reduction in biodiversity and a shift in composition; the…

0301 basic medicineGeologic SedimentsSub-surface marine sedimentMicrobial communitiealkB geneMicrobial ConsortiaBiodiversity010501 environmental sciencesAquatic ScienceSettore BIO/19 - Microbiologia GeneraleOceanography01 natural sciencesUnresolved complex mixtures (UCM)Clostridia03 medical and health sciencesRNA Ribosomal 16SMediterranean SeaPetroleum PollutionSicilyPhylogeny0105 earth and related environmental sciencesArcobacterBacteriabiologyDenaturing Gradient Gel ElectrophoresisSedimentBiodiversitySub-surface marine sediments; Microbial communities; alkB genes; Unresolved complex mixtures (UCM); Mediterranean Sea; Biodegradation; Arcobacterbiology.organism_classificationArchaeaPollutionHydrocarbonsPhylogenetic diversityBiodegradation EnvironmentalPetroleum030104 developmental biologyEnvironmental chemistryBiodegradationEnvironmental scienceProteobacteriaMicrocosmBayWater Pollutants ChemicalArchaeaMarine Pollution Bulletin
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Global, Regional, and National Cancer Incidence, Mortality, Years of Life Lost, Years Lived With Disability, and Disability-Adjusted Life-years for 3…

2017

Importance: Cancer is the second leading cause of death worldwide. Current estimates on the burden of cancer are needed for cancer control planning.Objective: To estimate mortality, incidence, years lived with disability (YLDs), years of life lost (YLLs), and disability-adjusted life-years (DALYs) for 32 cancers in 195 countries and territories from 1990 to 2015.Evidence Review: Cancer mortality was estimated using vital registration system data, cancer registry incidence data (transformed to mortality estimates using separately estimated mortality to incidence [MI] ratios), and verbal autopsy data. Cancer incidence was calculated by dividing mortality estimates through the modeled MI ratio…

0301 basic medicineGerontologyCancer ResearchPopulationArticle03 medical and health sciences0302 clinical medicineBreast cancerGlobal healthcancerMedicineDisability-adjusted life yeareducationDisease burdeneducation.field_of_studyestimate mortalityCancer preventioncancer preventionbusiness.industryMortality rate1. No povertymedicine.disease3. Good health030104 developmental biologyYears of potential life lostOncology030220 oncology & carcinogenesisbusinessearly diagnosisDemography
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Allosteric Cross-Talk among Spike’s Receptor-Binding Domain Mutations of the SARS-CoV-2 South African Variant Triggers an Effective Hijacking of Huma…

2021

The rapid and relentless emergence of novel highly transmissible SARS-CoV-2 variants, possibly decreasing vaccine efficacy, currently represents a formidable medical and societal challenge. These variants frequently hold mutations on the Spike protein's receptor-binding domain (RBD), which, binding to the angiotensin-converting enzyme 2 (ACE2) receptor, mediates viral entry into host cells. Here, all-atom molecular dynamics simulations and dynamical network theory of the wild-type and mutant RBD/ACE2 adducts disclose that while the N501Y mutation (UK variant) enhances the Spike's binding affinity toward ACE2, the concomitant N501Y, E484K, and K417N mutations (South African variant) aptly ad…

0301 basic medicineLetterMutantAllosteric regulationVirulenceBiologyMolecular Dynamics Simulationmedicine.disease_cause03 medical and health sciences0302 clinical medicineProtein DomainsViral entrymedicineHumansGeneral Materials SciencePhysical and Theoretical ChemistryReceptorchemistry.chemical_classificationGeneticsMutationSARS-CoV-2Antibodies Monoclonal030104 developmental biologyEnzymechemistrySettore CHIM/03 - Chimica Generale E InorganicaMutationSpike Glycoprotein Coronavirusbiology.proteinThermodynamicsAngiotensin-Converting Enzyme 2Antibody030217 neurology & neurosurgeryProtein Binding
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Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender.

2015

Gut microbiota plays a key role in multiple aspects of human health and disease, particularly in early life. Distortions of the gut microbiota have been found to correlate with fatal diseases in preterm infants, however, developmental patterns of gut microbiome and factors affecting the colonization progress in preterm infants remain unclear. The purpose of this prospective longitudinal study was to explore day-to-day gut microbiome patterns in preterm infants during their first 30 days of life in the neonatal intensive care unit (NICU) and investigate potential factors related to the development of the infant gut microbiome. A total of 378 stool samples were collected daily from 29 stable/…

0301 basic medicineMaleLongitudinal studyNeonatal intensive care unitPhysiologylcsh:MedicinePhysiologyGut floraPathology and Laboratory MedicineFamilies0302 clinical medicineAntibioticsMedicine and Health Scienceslcsh:ScienceChildrenBreast Milk2. Zero hungerMultidisciplinarybiologyAntimicrobialsMicrobiotaDrugsGenomicsBacterial PathogensBody FluidsIntestinesMilkMedical MicrobiologyFemaleInfant FoodPathogensAnatomyInfantsInfant PrematureResearch ArticleEnterobacterialesMicrobial GenomicsBreast milkMicrobiologyMicrobiology03 medical and health sciencesSex FactorsMicrobial ControlGeneticsHumansMicrobiomeMicrobial PathogensClostridiumPharmacologyBacterialcsh:RGut BacteriaInfant NewbornOrganismsBiology and Life SciencesNeonatesbiology.organism_classificationPostnatal age030104 developmental biologyAge GroupsPeople and Placeslcsh:QPopulation GroupingsMicrobiomeBacteroides030217 neurology & neurosurgeryDevelopmental BiologyPloS one
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Estimating Global Burden of Disease due to congenital anomaly: an analysis of European data

2017

ObjectiveTo validate the estimates of Global Burden of Disease (GBD) due to congenital anomaly for Europe by comparing infant mortality data collected by EUROCAT registries with the WHO Mortality Database, and by assessing the significance of stillbirths and terminations of pregnancy for fetal anomaly (TOPFA) in the interpretation of infant mortality statistics.Design, setting and outcome measuresEUROCAT is a network of congenital anomaly registries collecting data on live births, fetal deaths from 20 weeks’ gestation and TOPFA. Data from 29 registries in 19 countries were analysed for 2005–2009, and infant mortality (deaths of live births at age <1 year) compared with the WHO Mortality …

0301 basic medicineMalePediatrics030105 genetics & heredityInfant DeathGlobal Burden of Disease0302 clinical medicineCongenital anomaly ; DALY ; Global Burden of Disease ; YLL ; mortality.PregnancyPrenatal DiagnosisYLLEpidemiologyInfant MortalityPrevalenceMedicineEPIDEMIOLOGY030212 general & internal medicineRegistries1506DOWN-SYNDROMEPOPULATIONeducation.field_of_studyDALYAnomaly (natural sciences)Pregnancy OutcomeObstetrics and GynecologyGestational ageGeneral MedicineStillbirthUPDATED SYSTEMATIC ANALYSISPREVALENCEEuropeFetal Mortality/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_beingFemaleOriginal ArticleCHILD-MORTALITYAdultCOUNTRIESmedicine.medical_specialtyPopulationGestational AgeCongenital Abnormalities03 medical and health sciencesSDG 3 - Good Health and Well-beingJournal ArticleHumansCongenital anomalyAbortion Induced/statistics & numerical data; Adult; Congenital Abnormalities/diagnosis; Congenital Abnormalities/epidemiology; Europe/epidemiology; Female; Fetal Death/prevention & control; Fetal Mortality; Gestational Age; Global Burden of Disease/methods; Global Burden of Disease/statistics & numerical data; Humans; Infant; Infant Death/prevention & control; Infant Mortality; Infant Newborn; Male; Pregnancy; Pregnancy Outcome/epidemiology; Prenatal Diagnosis/methods; Prenatal Diagnosis/statistics & numerical data; Prevalence; Registries/statistics & numerical data; Stillbirth/epidemiology; Congenital anomaly; DALY; Global Burden of Disease; YLL; mortalityeducationFetal DeathPregnancybusiness.industryInfant NewbornInfantAbortion InducedNATIONAL CAUSESmedicine.diseasemortalityTRENDSInfant mortalityChild mortalityYears of potential life lostPediatrics Perinatology and Child HealthbusinessPRIMARY PREVENTIONDemography
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Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control.

2020

Contains fulltext : 218876.pdf (Publisher’s version ) (Open Access) OBJECTIVES: Conventional routine PCR testing for gastrointestinal infections is generally based on pathogen related panels specifically requested by clinicians and can be erroneous and time consuming. The BioFire FilmArray gastrointestinal (GI) panel combines 22 pathogens into a single cartridge-based test on a random-access system, thereby reducing the turnaround time to less than 2 hours. We described the clinical impact of implementing the BioFire FilmArray on patients with gastroenteritis in our hospital. METHODS: Patients attending a Dutch tertiary care center (Radboud University Medical Center), from whom stool sample…

0301 basic medicineMaleTime Factorslnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]Pathology and Laboratory MedicineTertiary carePolymerase Chain ReactionTertiary Care CentersFeces0302 clinical medicineClinical historyAntibioticsMedicine and Health SciencesMedicineInfection controlUniversity medicalGastrointestinal Infections030212 general & internal medicineChildNetherlandsAged 80 and overPotential impactMultidisciplinaryWomen's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17]AntimicrobialsQRDrugsGastrointestinal AnalysisMiddle AgedGastroenteritisBacterial PathogensBioassays and Physiological AnalysisMolecular Diagnostic TechniquesMedical MicrobiologyChild PreschoolViral PathogensVirusesMedicinePathogensResearch ArticleAdultmedicine.medical_specialtyIsolation (health care)AdolescentClostridium DifficileScience030106 microbiologyGastroenterology and HepatologyResearch and Analysis MethodsMicrobiologyPatient care03 medical and health sciencesYoung AdultDiagnostic MedicineInternal medicineMicrobial ControlHumansMicrobial PathogensAgedPharmacologyInfection ControlBacteriabusiness.industryGut BacteriaInfant NewbornOrganismsInfantBiology and Life SciencesPatient datalnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]Patient CarebusinessPloS one
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Association of metformin administration with gut microbiome dysbiosis in healthy volunteers

2018

Background Metformin is a widely used first-line drug for treatment of type 2 diabetes. Despite its advantages, metformin has variable therapeutic effects, contraindications, and side effects. Here, for the very first time, we investigate the short-term effect of metformin on the composition of healthy human gut microbiota. Methods We used an exploratory longitudinal study design in which the first sample from an individual was the control for further samples. Eighteen healthy individuals were treated with metformin (2 × 850 mg) for 7 days. Stool samples were collected at three time points: prior to administration, 24 hours and 7 days after metformin administration. Taxonomic composition of…

0301 basic medicineMaleendocrine system diseasesPhysiologylcsh:MedicineType 2 diabetesGut floraPathology and Laboratory MedicineOpportunistic Pathogens0302 clinical medicineRNA Ribosomal 16SMedicine and Health SciencesLongitudinal Studieslcsh:ScienceData ManagementMultidisciplinarybiologydigestive oral and skin physiologyHigh-Throughput Nucleotide SequencingGenomicsHealthy VolunteersMetformin3. Good healthMetforminBacterial PathogensTolerabilityMedical MicrobiologyFemalePathogensmedicine.drugResearch ArticleMicrobial TaxonomyAdultDNA BacterialEscherichiaComputer and Information SciencesClostridiaceae030209 endocrinology & metabolismMicrobial GenomicsPlaceboDNA RibosomalMicrobiologyDrug Administration Schedule03 medical and health sciencesYoung AdultEnterobacteriaceaeAdverse ReactionsmedicineGeneticsHumansMicrobiomeMicrobial PathogensTaxonomyPharmacologyClostridiumBacteriabusiness.industryPeptostreptococcusTherapeutic effectlcsh:RGut BacteriaOrganismsBiology and Life SciencesSequence Analysis DNAmedicine.diseasebiology.organism_classificationGastrointestinal Microbiome030104 developmental biologyDysbiosislcsh:QMicrobiomebusinessDysbiosisPLOS ONE
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GABA-containing compound gammapyrone protects against brain impairments in Alzheimer's disease model male rats and prevents mitochondrial dysfunction…

2018

Neuroinflammation, oxidative stress, decreased glucose/energy metabolism, and disrupted neurotransmission are changes that occur early in sporadic Alzheimer's disease (AD), manifesting as mild cognitive impairment. Recently, the imbalanced function of the gamma-aminobutyric acid (GABA) system was identified as a critical factor in AD progression. Thus, maintaining balance among neurotransmitter systems, particularly the GABA system, can be considered a beneficial strategy to slow AD progression. The present study investigated the effects of the compound gammapyrone, a molecule containing three GABA moieties: "free" moiety attached to the position 4 of the 1,4-dihydropyridine (DHP) ring, and…

0301 basic medicineMalemedicine.medical_specialtyAllosteric regulationbioenergetics; GABA; intracerebroventricular streptozocin; PC12 cells; protein expression; spatial learning/memoryNeurotransmissionspatial learning/memorymedicine.disease_causebioenergeticsNeuroprotection03 medical and health sciencesCellular and Molecular NeuroscienceGABA0302 clinical medicineReceptors GABAAlzheimer DiseaseMemoryInternal medicinemedicineAnimalsRats WistarReceptorMaze Learningprotein expressionNeuroinflammationCells Culturedgamma-Aminobutyric AcidGABAA receptorChemistryGlutamate DecarboxylasePC12 cellsBrainintracerebroventricular streptozocinMitochondriaStreptozocinDisease Models Animal030104 developmental biologyEndocrinologyNeuroprotective AgentsAstrocytesAcetylcholinesteraseEncephalitisMicroglia030217 neurology & neurosurgeryOxidative stressJournal of neuroscience research
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Mismatch or allostatic load? Timing of life adversity differentially shapes gray matter volume and anxious temperament

2015

Traditionally, adversity was defined as the accumulation of environmental events (allostatic load). Recently however, a mismatch between the early and the later (adult) environment (mismatch) has been hypothesized to be critical for disease development, a hypothesis that has not yet been tested explicitly in humans. We explored the impact of timing of life adversity (childhood and past year) on anxiety and depression levels (N = 833) and brain morphology (N = 129). Both remote (childhood) and proximal (recent) adversities were differentially mirrored in morphometric changes in areas critically involved in emotional processing (i.e. amygdala/hippocampus, dorsal anterior cingulate cortex, res…

0301 basic medicineMalestressful life eventschildhood maltreatmentEmotionsAnxietySocial EnvironmentDevelopmental psychology0302 clinical medicineGray MatterVBMChildadversitymedia_commonDepressionAdult Survivors of Child AbuseAllostasisBrainGeneral MedicineOrgan SizeMagnetic Resonance ImagingAllostatic loadmedicine.anatomical_structureAllostasisAnxietyFemalemedicine.symptomPsychologymismatchallostatic loadAdultCognitive Neurosciencemedia_common.quotation_subjectExperimental and Cognitive PsychologyAffect (psychology)AmygdalaLife Change Events03 medical and health sciencesYoung AdultmedicineHumansddc:610TemperamentAnterior cingulate cortexBrain morphometryOriginal ArticlesImage Enhancement030104 developmental biologyTemperament030217 neurology & neurosurgerySocial Cognitive and Affective Neuroscience
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Mycobacterium avium subsp. paratuberculosis (Map) Fatty Acids Profile Is Strain-Dependent and Changes Upon Host Macrophages Infection

2017

Johne´s disease is a chronic granulomatous enteritis of ruminants caused by the intracellular bacterium Mycobacterium avium subsp. paratuberculosis (Map). We previously demonstrated that Map isolates from sheep persisted within host macrophages in lower CFUs than cattle isolates after 7 days of infection. In the current study, we hypothesize that these phenotypic differences between Map isolates may be driven be the fatty acids (FAs) present on the phosphadidyl-1-myo-inositol mannosides of the Map cell wall that mediate recognition by the mannose receptors of host macrophages. FAs modifications may influence Map´s envelope fluidity ultimately affecting pathogenicity. To test this hypothesis…

0301 basic medicineMicrobiology (medical)Mycobacterium avium subp. paratuberculosis030106 microbiologyImmunologyParatuberculosisTuberculostearic acidBiologyMicrobiologyfatty acidsMicrobiologyCell wall03 medical and health scienceschemistry.chemical_compoundlipid metabolismmedicineExtracellularMacrophageMap-host interactionOriginal ResearchIntracellular parasitemedicine.diseasebiology.organism_classificationmacrophages030104 developmental biologyInfectious DiseaseschemistryBacteriaMycobacteriumFrontiers in Cellular and Infection Microbiology
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