Search results for "mapping"
showing 10 items of 1508 documents
Production of biologically active light chain of tetanus toxin inEscherichia coli
1993
AbstractThe activity of the light (L) chain of tetanus toxin, and of mutants constructed by site-directed mutagenesis, was studied by expression and purification of the proteins from E. coli. Wild-type recombinant L chain (pTet87) was active in the inhibition of exocytosis from cultured bovine adrenal chromaffin cells, although at a level 5–15% of that of L chain purified from tetanus toxin. L chain mutants which terminated at Leu-438 (pTet89), or which contained a Cys-to-Ser mutation at residue 439 (pTet88) were equally as active as the full-length recombinant protein. The reduced activity of pTet87 L chain correlated with C-terminal proteolysis of the protein upon purification. A tryptic …
Comparative cytogenetics of human chromosome 3q21.3 reveals a hot spot for ectopic recombination in hominoid evolution
2004
Fluorescence in situ hybridization mapping of fully integrated human BAC clones to primate chromosomes, combined with precise breakpoint localization by PCR analysis of flow-sorted chromosomes, was used to analyze the evolutionary rearrangements of the human 3q21.3-syntenic region in orangutan, siamang gibbon, and silvered-leaf monkey. Three independent evolutionary breakpoints were localized within a 230-kb segment contained in BACs RP11-93K22 and RP11-77P16. Approximately 200 kb of the human 3q21.3 sequence was not present on the homologous orangutan, siamang, and Old World monkey chromosomes, suggesting a genomic DNA insertion into the breakpoint region in the lineage leading to humans a…
Una aproximación volumétrica a la desagregación espacial de la población combinando cartografía temática y datos LIDAR
2016
[EN] Availability of high resolution population distribution data, independent of the administrative units in which demographic statistics are collected, is a real necessity in many fields: risk evaluation due to earthquakes, flooding or fires, to name just a few, integration between socio-demographic and environmental or geographical information collected in different formats, policy design for the provision public services, such as health, education or public transport, or mobility studies in urban areas or metropolitan regions. Because of this, the literature has explored various methods of population downscaling, collected at communality or census tract level, into smaller areas; typica…
A volumetric approach to spatial population disaggregation using a raster build-up layer, land use/land cover databases (SIOSE) and LIDAR remote sens…
2016
[EN] Availability of high resolution population distribution data, independent of the administrative units in which demographic statistics are collected, is a real necessity in many fields: risk evaluation due to earthquakes, flooding or fires, to name just a few, integration between socio-demographic and environmental or geographical information collected in different formats, policy design for the provision public services, such as health, education or public transport, or mobility studies in urban areas or metropolitan regions. Because of this, the literature has explored various methods of population downscaling, collected at communality or census tract level, into smaller areas; typica…
Finding Factors Influencing Students’ Preferences to Concept Mapping Tasks: Literature Review
2014
Abstract There is a broad set of concept mapping tasks which can be used by teachers for assessing students’ knowledge structures but there are no known works of research studying their adaptation to students’ individual differences and preferences in relation to different tasks. The paper reports first results of activities which aim to offer a solution of the abovementioned problem. It is based on extensive review of theoretical and empirical studies and focuses on: a) identification of advantages and drawbacks of different concept mapping tasks, and b) finding factors which may affect students’ concept mapping ability.
Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
2019
Background This study aimed to evaluate remineralizing ability of two hydraulic calcium-silicate cements (Biodentine and TheraCal LC). Material and Methods Artificial carious lesions were introduced into the pulpal floors (1-1.5 mm) and axial walls of occlusal prepared cavity halves through pH cycling. Cycling was made through demineralizing solution (pH 3), for 8 hours and remineralizing solution (pH 7) for 16 hours. The total period of pH cycling was 14 days. Prepared cavities with the tested materials seated directly on the pulpal floor and in contact with the axial walls were stored in phosphate buffer solutions (PBS) (pH 7.2–7.4). The changes in the weight percentages (wt%) of calcium …
Digital orthophotos at a scale of 1:5000 from high resolution satellite images
2004
This paper describes the first results of a study, carried out within the national research program “Cofin2001”, on the potential use of high resolution satellite images for making digital orthophotos at a scale of 1:5000 and 1:10,000. Particularly, images from IKONOS II and QuickBird of four different types of areas were employed: the first area is the city of Palermo, a greatly urbanized area; the second area is the extra-urban territory near the town of Contessa Entellina in south-western Sicily; the third area is the famous tourist resort of Scopello, in the western Sicily; the last area is the Caselle Airport, a flat zone near Torino. The first three images were orthorectified by using…
Mapping and structure of DMXL1, a human homologue of the DmX gene from Drosophila melanogaster coding for a WD repeat protein.
2000
The DmX gene was recently isolated from the X chromosome of Drosophila melanogaster. TBLASTN searches of the dbEST databases revealed sequences with a high level of similarity to DmX in a variety of different species, including insects, nematodes, and mammals showing that DmX is an evolutionarily highly conserved gene. Here we describe the cloning of the cDNA and the chromosomal localization of one of the human homologues of DmX, Dmx-like 1 (DMXL1). The human DMXL1 gene codes for a large mRNA of 11 kb with an open reading frame of 3027 amino acids. The putative protein belongs to the superfamily of WD repeat proteins, which have mostly regulatory functions. The DMXL1 protein contains an exc…
Simple Strategic Analysis Tools at SMEs in Ecuador
2015
This article explores the possible applications of Strategic Analysis Tools (SAT) in SMEs located in emerging countries such as Ecuador (where there are no formal studies on the subject). It is intended to analyze if whether or not it is feasible to effectively apply a set of proposed tools to guide mental map decisions of executives when decisions on strategy have to be made. Through an in-depth review of the state of the art in regards to SAT and interviews performed to main participants such as chambers and executives of different firms, it is shown the feasibility of their application. This analysis is complemented with specialists´ interviews to deepen our insights and obtaining valid …
A systematic concept analysis of mental health promotion
2016
AbstractThis study explored and clarified the nature and characteristics of the concept of mental health promotion. The study also investigated how these characteristics appear in current policies and strategies. A total of 30 scientific articles and policy documents were identified and analysed using Rodgers’s systematic evolutionary concept analysis method. The analysis provided valuable information on the attributes, related concepts, antecedents, consequences and references of mental health promotion, indicating that the concept is a distinct concept comprising a unique set of attributes and characteristics. A concept mapping of mental health promotion was subsequently developed. The an…