Search results for "marker"

CARATTERIZZAZIONE MOLECOLARE DI BATTERI IN REPERTI LIGNEI SOMMERSI

2013

The focus of this study was the identification of bacterial colonies in waterlogged wood fragments from the rostrum of a excellent workmanship, that is very likely one of the wrecks attributed to Sextus Pompey fleet (36 BC) and discovered in Acqualadroni, Messina, Sicily, Italy (2008). The wood samples were analyzed by light and Scanning Electron Microscopy (SEM), in vitro culture and molecular technique (DNA base techniques). The results, focused on bacterial consortia, allowed us to reveal the presence of Pseudomonas sp., Sphingomonas sp., Xanthomonas sp. besides Marinobacter sp. and Desulforudis audaxviator. A prompt and accurate characterization of bacterial colonization represents one …

Functional investigations of matrix metalloproteinases in breast cancer: focus on MMP9 and MMP2

2021

Effect of sodium dodecyl sulfate (SDS) on stress response in the Mediterranean mussel (Mytilus Galloprovincialis): Regulatory volumedecrease (Rvd) an…

2014

In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are assessed on the Mediterranean mussel (. Mytilus galloprovincialis), exposed for 18 days at a concentration ranging from 0.1. mg/l to 1. mg/l. The effects are monitored using biomarkers related to stress response, such as regulatory volume decrease (RVD), and to oxidative stress, such as reactive oxygen species (ROS), endogenous antioxidant systems and Hsp70 levels. The results demonstrate that cells from the digestive gland of M. galloprovincialis, exposed to SDS were not able to perform the RVD owing to osmotic stress. Further, SDS causes oxidative stress in treated organisms, as demonstrated by the incre…

Do island plant populations really have lower genetic variation than mainland populations? Effects of selection and distribution range on genetic div…

2015

Ecological and evolutionary studies largely assume that island populations display low levels of neutral genetic variation. However, this notion has only been formally tested in a few cases involving plant taxa, and the confounding effect of selection on genetic diversity (GD) estimates based on putatively neutral markers has typically been overlooked. Here, we generated nuclear microsatellite and plastid DNA sequence data in Periploca laevigata, a plant taxon with an island–mainland distribution area, to (i) investigate whether selection affects GD estimates of populations across contrasting habitats; and (ii) test the long-standing idea that island populations have lower GD than their mai…

Analysis of lipids in pelagic fish of Sicilian Channel and use of fatty acids as bacterial contamination markers

Lipids provide the expendable form of energy in marine ecosystem, and have an important role for the reproductive biology of fish. Also, lipids are important nutrients, versatile markers for marine trophic studies, bacteria markers etc. Among the lipids, several essential fatty acids are determinants for ecosystem health as well as for their known nutritional properties and prevention capacity of chronic diseases. Furan fatty acids (F - acids) are other important lipid bioactive compounds; for these is hypothesized a crucial nutritional role that is synergistic to polyunsaturated fatty acids omega 3 (PUFA ω 3), also if their mechanism is not yet clear. Source of essential fatty acid is the …

Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei

1999

Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei's index, showed a value of 0.413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.

Screening of ecotoxicological, qualitative and reproductive variables in male European sea bassDicentrarchus labrax(L.) reared in three different fis…

2013

The aim of this work was to do a preliminary seasonal screening of ecotoxicological biomarkers in European sea bass Dicentrarchus labrax in three different fish farms, to know if the different location and typology can discriminate them. A set of selected biomarkers of xenobiotic exposure, such as acetylcholinesterase (AChE) activity, Glutathione (GSH) and gonad morphology were investigated seasonally in male European sea bass D. labrax (L.) reared in three different intensive farms: a land-based farm of cement tanks (T), an in-shore sea cages farm (C1) and an off-shore sea cages farm (C2). The results showed that both location and typology can discriminate AChE activity, GSH content and go…

Assessing vermetid reefs as indicators of past sea levels in the Mediterranean

2020

Abstract The endemic Mediterranean reef building vermetid gastropods Dendropoma petraeum complex (Dendropoma spp) and Vermetus triquetrus develop bio-constructions (rims) on rocky shorelines at about Mean Sea Level (MSL) and are therefore commonly used as relative sea-level (RSL) markers. In this study, we use elevations and age data of vermetid reefs to (1) re-assess the vertical uncertainties of these biological RSL indicators, and (2) evaluate the vertical growth rates along a Mediterranean east-west transect, in attempt to explain the differences found in both growth rates and uncertainties. In Israel, Differential Global Positioning System (DGPS) and laser measurements relative to the …

Genetic discontinuities within the Mediterranean basin. Post-glacial recolonization patterns and recent current regime

2009

Immediate biomarker responses to benzo[a]pyrene in polluted and unpolluted populations of the blue mussel (Mytilus edulis L.) at high-latitudes.

2008

Immediate biomarker responses of two high-latitude populations of the blue mussel Mytilus edulis to benzo[a]pyrene (B[a]P) were evaluated. Mussels collected from a clean and a polluted site in southwest Iceland were exposed to the nominal dose of 100 μg B[a]P L−l for 3 h, after 4 days of acclimatization in clean seawater. To test the sensitivity to the toxicant and immediate biological responses, the following biomarkers were used: DNA single strand breaks, heart rate and feeding rate.All the biomarkers revealed differences between the study sites. Irrespective of the origin of the organisms, the short time exposure to the high B[a]P concentration did not induce DNA single strand breaks or …