Search results for "marker"
showing 10 items of 3799 documents
Simple Sequence Repeat Marker Development and Mapping Targeted to Previously Unmapped Regions of the Strawberry Genome Sequence
2011
The genome sequence of the woodland strawberry (Fragaria vesca L.) is an important resource providing a reference for comparative genomics studies and future sequenced rosaceous species and has great utility as a model for the development of markers for mapping in the cultivated strawberry Fragaria ×ananassa Duchesne ex Rozier. A set of 152 microsatellite simple sequence repeat (SSR) primer pairs was developed and mapped, along with 42 previously published but unmapped SSRs, permitting the precise assignment of 28.2 Mbp of previously unanchored genome sequence scaffolds (13% of the F. vesca genome sequence). The original ordering of F. vesca sequence scaffolds was performed without a physic…
Explicit Oral Narrative Intervention for Students with Williams Syndrome
2018
Narrative skills play a crucial role in organizing experience, facilitating social interaction and building academic discourse and literacy. They are at the interface of cognitive, social, and linguistic abilities related to school engagement. Despite their relative strengths in social and grammatical skills, students with Williams syndrome (WS) do not show parallel cognitive and pragmatic performance in narrative generation tasks. The aim of the present study was to assess retelling of a TV cartoon tale and the effect of an individualized explicit instruction of the narrative structure. Participants included eight students with WS who attended different special education levels. Narratives…
Spheroids of adipose derived stem cells show their potential in differentiating towards the angiogenic lineage
2023
Introduction: Adipose derived stem cells (ASCs) are a mesenchymal stem cell population of great scientific interest due to their abundance and easiness in obtaining them from adipose tissue. Recently, several techniques for three dimensional (3D) ASCs cultivation have been developed to obtain spheroids of adipose stem cells (SASCs). It was already proved that ASCs are able to differentiate towards the endothelial lineage thus, for the first time, we investigated the ability of our 3D SASCs to differentiate endothelially and the effects of not differentiated SASC secreted factors on specific cultured cells. Materials and methods: SASCs were differentiated with a specific medium towards endot…
Expression of the human XPB/ERCC-3 excision repair gene-homolog in the sponge Geodia cydonium after exposure to ultraviolet radiation.
1998
Abstract The marine demosponge Geodia cydonium encodes a gene, termed GCXPB , which displays 62% identity to the human XPB/ERCC-3 gene that specifically corrects the repair defect in xeroderma pigmentosum and in Cockayne's syndrome. The cDNA was isolated and characterized the deduced aa sequence, XPB_GEOCY, with the calculated size of 91,541 Da comprises the characteristic domains found in the related helicases. Phylogenetic tree analysis revealed that the sponge sequence is grouped to the metazoan related XPB/ERCC-3 polypeptides. Northern Blot analyses have been performed with sponge samples collected at different depths, thus exposed to different intensities of UV sunlight in the field. T…
Distinct Clones of Yersinia pestis Caused the Black Death
2010
From AD 1347 to AD 1353, the Black Death killed tens of millions of people in Europe, leaving misery and devastation in its wake, with successive epidemics ravaging the continent until the 18th century. The etiology of this disease has remained highly controversial, ranging from claims based on genetics and the historical descriptions of symptoms that it was caused by Yersinia pestis to conclusions that it must have been caused by other pathogens. It has also been disputed whether plague had the same etiology in northern and southern Europe. Here we identified DNA and protein signatures specific for Y. pestis in human skeletons from mass graves in northern, central and southern Europe that …
The 2016 CEOS Infrared Radiometer Comparison: Part II: Laboratory Comparison of Radiation Thermometers
2019
AbstractTo ensure confidence, measurements carried out by imaging radiometers mounted on satellites require robust validation using “fiducial quality” measurements of the same in situ parameter. For surface temperature measurements this is optimally carried out by radiometers measuring radiation emitted in the infrared region of the spectrum, collocated to that of a satellite overpass. For ocean surface temperatures the radiometers are usually on board ships to sample large areas but for land and ice they are typically deployed at defined geographical sites. It is of course critical that the validation measurements and associated instrumentation are internationally consistent and traceable …
A new tool to assess the ecotoxicological impact of β-triketone herbicides on soil microbial communities
2018
International audience; The β-triketone herbicides are post-emergence maize selective herbicides that have beenintroduced on the market, in replacement of atrazine, banned in Europe in 2004. Qualified as “eco-friendly”, since they are based on natural phytotoxin properties, these herbicides target an enzymeinvolved in carotenoid biosynthesis called 4-hydroxyphenylpyruvate dioxygenase (HPPD) encoded bythe hppd gene. The inhibition of this enzyme provokes bleaching symptoms, necrosis and death ofweeds.The hppd gene is not only find in eukaryotes such as plants, animals and humans but also inprokaryotes such as fungi, yeasts and bacteria. In recent studies, we showed that, within the soil bact…
Molecular characterization of cyclic and obligate parthenogens in the aphid Rhopalosiphum padi (L.)
1996
Holocyclic clones of the aphid Rhopalosiphum padi (L.) reproduce by cyclic parthenogenesis, whereas anholocyclic individuals are obligate parthenogens. Mitochondrial DNA (mtDNA) and random amplified polymorphic DNA markers in R . padi as well as plasmid DNA markers of its bacterial endosymbiont, Buchnera aphidicola , were examined to determine the extent of genetic divergence between clones with these differing breeding systems. These analyses revealed that cyclically parthenogenetic lineages possessed differing mtDNA and plasmid haplotypes than most obligately asexual clones. The extent of sequence divergence between these maternally inherited molecules suggests a relatively ancient origin…
Diversité génétique et aromatique de la truffe de Bourgogne
2013
Truffles are ectomycorrhizal Ascomycota fungi belonging to the Tuberaceae family and more specifically to the Tuber genus. More than thirty Tuber species are currently described in Europe. In this thesis, we specifically focused on the Tuber aestivum-uncinatum model. This truffle is commonly called "Burgundy Truffle" and has a gastronomic and cultural interest.The first part of this thesis focused on the taxonomic status of the Burgundy truffle (Tuber uncinatum). For this, we used a multi-marker approach combining several genetic markers commonly used at the interspecific scale. Our analyses showed that the two taxa, Tuber aestivum (summer truffle) and Tuber uncinatum are conspecific.In the…
New polymorphic markers for genetic diversity studies in an invasive plant: the common ragweed (Ambrosia artemisiifolia L.)
2014
Common ragweed is an annual herb that belongs to the Asteraceae family. It is known as an invasive plant originating from the USA. In France the common ragweed is especially abundant in the Rhône-Alpes basin and currently spreads northwards in Burgundy. Ragweed colonizes different types of environments, such as railways, river sides, wastelands, farmlands and cultivated crops (especially sunflowers crops). Herbicide resistance to linuron, glyphosate and/or acetolactate synthase inhibitors has been reported in the USA, which complicates the chemical control of ragweed. In addition, the highly allergenic pollen of ragweed causes severe allergies. These elements make this plant a major threat …