Search results for "medium"
showing 10 items of 3746 documents
Conditioned Medium from Human Amnion-Derived Mesenchymal Stromal/Stem Cells Attenuating the Effects of Cold Ischemia-Reperfusion Injury in an In Vitr…
2021
The clinical results of lung transplantation (LTx) are still less favorable than other solid organ transplants in both the early and long term. The fragility of the lungs limits the procurement rate and can favor the occurrence of ischemia-reperfusion injury (IRI). Ex vivo lung perfusion (EVLP) with Steen SolutionTM (SS) aims to address problems, and the implementation of EVLP to alleviate the activation of IRI-mediated processes has been achieved using mesenchymal stromal/stem cell (MSC)-based treatments. In this study, we investigated the paracrine effects of human amnion-derived MSCs (hAMSCs) in an in vitro model of lung IRI that includes cold ischemia and normothermic EVLP. We found tha…
ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
2017
Growth phenotypes of microorganisms are a strong indicator of their underlying genetic fitness and can be segregated into 3 growth regimes: lag-phase, log-phase, and stationary-phase. Each growth phase can reveal different aspects of fitness that are related to various environmental and genetic conditions. High-resolution and quantitative measurements of all 3 phases of growth are generally difficult to obtain. Here we present a detailed method to characterize all 3 growth phases on solid media using an assay called One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). ODELAY quantifies growth phenotypes of individual cells growing into colonies on solid media using time-lapse mi…
The effects of fractional CO2 laser, Nano-hydroxyapatite and MI paste on mechanical properties of bovine enamel after bleaching
2017
Background This study investigated the effect of post bleaching treatments to the change of enamel elastic modulus and microhardness after dental bleaching in- vitro. Material and Methods Fifty bovine incisor slab were randomly assigned into five groups (n=10). The samples were bleached for three times; 20 minutes each time, by 40% hydrogen peroxide. Next it was applied fractional CO2 laser for two minutes, Nano- hydroxy apatite (N-HA) and MI-paste for 7 days and 2 minutes per day. The sound enamel and bleached teeth without post treatment remained as control groups. The elastic modulus and microhardness were measured at three times; 24 hours, 1 and 2 months. Data were statistically analyze…
Stability of vacuolar betaxanthin pigments in juices from Moroccan yellow Opuntia ficus indica fruits
2008
Pigment stability of yellow juices prepared from Moroccan cactus pear (Opuntia ficus indica) was determined as a function of temperature and pH. The experiments were carried out at temperatures ranging from 80 to 100 °C with juices at pH 3.5, 5 and 6.5. The degree of pigment retention decreased when the temperature increased. The degradation constant rates were determined for thermal degradation rates of pseudo-first order. The Arrhenius plot obtained for the degradation of betaxanthin from the yellow fruits was not linear. Regardless of the temperature treatment, the lowest degradation was obtained for pH 5. Where some stabilizers were tested for the protection of pigments, the results sho…
Comparative evaluation of bioactive cements on biomimetic remineralization of dentin
2020
Background This study was designed to quantitatively compare the presence of apatite peaks on demineralized dentin to induced bio-mimetically by bioactive commercial materials. Material and methods Dentin slice specimens (n=6) were prepared and demineralized by by17% EDTA for 2 hours. Each disks materials (Theracal, Biodentine, CPP-ACP) were freshly prepared and was maintained in close contact with a demineralized dentin specimen immersed in PBS solution during one week. To evaluation of bioactivity, cements disks (n=6) were prepared from each material and immersed in PBS solution for one week. The bioactivity and remineralization ability was evaluated using FTIR spectroscopy and Scanning E…
Synthesis and influence of fatty acid esters on the foliar penetration of herbicides
1998
Abstract Synthesis of mono-esters of tetraethylene glycol and di-esters of tetraethylene glycol was accomplished from fatty acids and tetraethylene glycol in the absence of solvent and catalyst. Esterification was studied in a batch reactor by using a response surface methodology to evaluate the influence of synthesis variables such as molar substrate ratio of fatty acid relative to tetraethylene glycol (1.39–7.52), temperature (145–167°C), reaction time (0.5–8 h) on the yield of mono- and di-esters of tetraethylene glycol. A multipurpose reactor was developed for the transformation of fatty acids into pure α-monoglycerides. The 1-monoglycerides of heptanoic, undecelenic, oleic, linoleic an…
Use of Wood Ash in the Forest and its Effect on the Concentration of Essential and Heavy Metallic Elements in Soil and Blueberries <i>(Vacciniu…
2020
The aim of this research is to evaluate the influence of wood ash as an additive fertilizer to forest soil and growing blueberries (Vaccinium myrtillus L.) in terms of metallic element content. To evaluate the effect of wood ash on the forest ecosystem, was selected two forest areas which was fertilized with a certain amount of wood ash (bottom ash and fly ash). The selected forest areas were divided into two types of sampling plots (fertilized with ash and control). Obtained results showed that in the bottom ash dispersion area the concentration of Ca, K, Ni, Cu is almost twice higher in the fertilized plots than control plots. In the blueberries, metallic element content is similar and do…
What makes environmental performance differ between firms? Empirical evidence from the Spanish tile industry
2007
Environmental performance is a matter of major concern both for policy makers and for firm managers. In this paper we interpret firms' environmental performance as their ability to reduce polluting wastes while maintaining observed levels of inputs and desirable outputs. Making use of data envelopment analysis techniques, we compute waste-specific environmental efficiency measures for a sample of ceramic-tile producers located in the eastern Spanish region of Valencia. Our results show that there exists substantial room for improving environmental performance, which would have highly beneficial consequences for the local environment. In a second stage of analysis, we find that affiliation …
Transforming student contributions into subject-specific expression
2021
Drawing on a corpus of pre-service teacher training classroom interactions in an English-medium instruction university in Turkey, we examine teacher follow-up turns that introduce specialized terms, showing how a teacher transforms student’s responses into pedagogically relevant points using academic language. We argue that teacher third-turns following student contributions accomplish several interrelated actions, not only introducing new terminology to these teachers-in-training, but also familiarizing them with ways of thinking specific to their discipline, i.e., these turns model “doing being a teacher.” These teacher actions are used to bridge student contributions to more scientific t…
‘It has given me this kind of courage…’: the significance of CLIL in forming a positive target language self-concept
2019
This article sets out to broaden the understanding of foreign language self-concept in CLIL context. The few existing studies on self-concept in CLIL have been quantitative and provided somewhat discrepant results, highlighting the need for approaching the topic qualitatively. The data of the present study are in-depth interviews with 24 former Finnish CLIL pupils who retrospectively reflected on their CLIL experiences. The participants had received English-medium CLIL for nine years during their comprehensive school in the 1990s. The data were analyzed qualitatively using thematic analysis. The participants generally felt that CLIL had had a significant role in contributing to their very p…