Search results for "metabolite"
showing 10 items of 551 documents
Postmortem distribution of dihydrocodeine and metabolites in a fatal case of dihydrocodeine intoxication.
1998
A report of a fatal dihydrocodeine ingestion under substitution therapy is given. Quantitation of dihydrocodeine, dihydromorphine, N-nordihydrocodeine, dihydrocodeine-6-, dihydromorphine-6- and dihydromorphine-3-glucuronide was performed simultaneously after solid-phase extraction prior to HPLC analysis, and the analytes were detected using their native fluorescence. Postmortem concentrations of blood samples from different sampling sites as well as from liver, kidney and cerebrum are reported. A hair sample was investigated to prove long-term use of the substitute drug. Site-to-site differences of the analytes from blood samples were very small. The partition behavior of the opioid glucuro…
Composition of mosses, their metabolites and environmental stress impacts
2018
Sūnas ir nozīmīga augu grupa ar plašu izplatību. Sūnu sastāva pētījumi ir kļuvuši aktuāli, ņemot vērā bioloģiski aktīvu savienojumu klātbūtni un augsto izturību pret vides apstākļu mainību. Promocijas darba mērķis ir analizēt sūnu sastāvu, to metabolītus un vides stresa ietekmes uz tiem. Veikta sūnu sekundāro metabolītu ekstrakcijas apstākļu izpēte, kas nodrošina liela skaita vielu identifikāciju. Sūnu lipīdi satur daudzas bioloģiski aktīvas vielas un veikta to aktivitātes izpēte. Sūnu sekundārie metabolīti uzrāda augstu antimikrobiālo aktivitāti un spējas inhibēt vairāku vēža šūnu līniju attīstību un līdz ar to satur perspektīvas vielu grupas izmantošanai biomedicīnā. Vides stresa ietekmes…
The use of fish bile metabolite analyses as exposure biomarkers to pulp and paper mill effluents
1998
The exposure of caged one-year-old whitefish (Coregonus lavaretus) to wood extractives and chlorophenolics from pulp and paper mill effluents in Lake Saimaa (SE Finland) was investigated. Whitefish were exposed at 17 locations affected by effluents from pulp and paper mills and at 5 reference areas. Resin and fatty acids and chlorophenolics in bile were analysed by GC and GC-MS. Cholesterol concentration in bile was also measured. Results show that despite of process changes to ECF and waste water treatment improvements employed by the pulp and paper industry in the study area, it is still possible to detect elevated concentrations of these substances in the bile of fish exposed in the vici…
Reaktive Metabolite cancerogener polycyclischer Kohlenwasserstoffe: Synthese und Abfangreaktion von 9-Hydroxybenzo[a]pyren-4,5-oxid
1985
Le compose du titre est prepare a partir de l'acetate-9 de benzo [a] pyrene et piege par l'ethanethiol. Mecanismes
A bacterial metabolite, trimethylamine N-oxide, disrupts the hemostasis balance in human primary endothelial cells but no coagulopathy in mice
2019
: The gut microbial metabolite, trimethylamine N-oxide (TMAO), was previously reported to induce platelet hypersensitivity, which leads to thrombotic risk. However, the molecular mechanism underlying the effects of TMAO on endothelial cells (EC), which is the primary vessel wall contact with the lumen, remains unclear. Here, we investigated the impact of TMAO on procoagulant activity (PCA) in EC and mice, for a possible link between microbiota and coagulation. To test the PCA of TMAO in EC, we performed one-stage clotting assays and converted into PCA. Antitissue factor (TF) antibody was used to test the TF role in PCA. Quantitative PCR was performed to measure the TF, thrombomodulin, IL-6,…
Evaluation of carbamazepine uptake and metabolization by Typha spp., a plant with potential use in phytotreatment
2011
Abstract Phytoremediation technologies such as constructed wetlands have shown higher efficiencies in removal of pharmaceuticals from wastewaters than conventional wastewater treatment processes, and plants seem to have an important role in the removal of some of those compounds. In this context, a study was conducted to assess tolerance, uptake, and metabolism of the epilepsy drug, carbamazepine, by the macrophyte Typha spp. This evaluation was conducted in hydroponic solutions with 0.5–2.0 mg/L of this pharmaceutical for a maximum period of 21 days. The removal of carbamazepine from nutrient solutions by the plants reached values of 82% of the initial contents. Furthermore, a metabolite (…
Modulation of the control of mutagenic metabolites derived from cyclophosphamide and ifosfamide by stimulation of protein kinase A
1990
The phosphorylation of the 2 major phenobarbital-inducible cytochrome P450 isoenzymes IIB1 and IIB2 was increased in intact hepatocytes by the action of the membrane-permeating cAMP derivative N6,O2'-dibutyryl-cAMP. Under these conditions cyclophosphamide and ifosfamide (which are known to be activated by cytochrome P450 IIB1) were investigated for mutagenicity in Salmonella typhimurium TA1535 and TA100 and for cytotoxicity in TA1535. Cyclophosphamide and ifosfamide were transformed to mutagenic and cytotoxic metabolites by the hepatocytes. The activation of both drugs to mutagens was markedly reduced after pretreatment of the hepatocytes with the membrane-permeating cAMP derivative N6,O2'-…
Weekly oxaliplatin, high-dose folinic acid and 24h-5-fluorouracil (FUFOX) as salvage therapy in metastatic colorectal cancer patients pretreated with…
2002
Irinotecan (CPT-11), oxaliplatin (OXA) and different folinic acid (FA) modulated 5-fluorouracil (5-FU) regimens are active as first-and second-line chemotherapy of metastatic colorectal cancer. However, the best palliative sequence of these substances is still unclear. After CPT-11 containing regimens the optimal salvage protocol has not yet been defined. Here, we retrospectively analysed the weekly ambulant combination of OXA with continuous FA/5-FU (FUFOX) after two different CPT-11 containing chemotherapeutic regimens. Patients: During October 1999 and May 2001, 20 patients (median 62; 48-74 years) were included who had disease progression after CPT-11/bolus FA/5-FU (Saltz; 7 patients, g…
Sensitive and selective determination of metabolically formed trans -dihydrodiols and phenols of benzo[ a ]pyrene in water and urine samples by HPLC …
1998
A method has been developed to separate hydroxylated metabolites of the carcinogenic polycyclic aromatic hydrocarbon benzo[a]pyrene, i. e. trans-4,5-, 7,8-, 9,10-dihydrodiol and 1-, 3-, 7-, and 9-phenol, by HPLC with amperometric detection employing an isocratic methanol/water eluent (70:30, v/v) containing 0.5 g/L sulfuric acid and 1 g/L lithium perchlorate. Compared with the usually applied fluorescence (λex = 265 nm, λem = 460 nm) and ultraviolet (λ = 265 nm) detection, the amperometric technique is about 2–12 times more sensitive for the determination of all metabolites investigated. The method was applied to the determination of the seven metabolites of benzo[a]pyrene in different wate…
In vivoInvestigation of Plant-Cell Metabolism by means of natural-abundance13C-NMR spectroscopy
1994
Based on the natural abundance of 13C, in vivo13C-NMR was used for the first time to monitor the metabolism of sucrose and hydroquinone (1) in cell suspensions of the plant Rauwolfia serpentina (L.) BENTH. ex KURZ. Cells converted sucrose extracellularly into α-D- and β -D-glucose as well as into β -D-fructofuranose and β -D-fructopyranose, respectively. The sugar mixture was completely taken up by the cells after 4 days. Hydroquinone fed at that time resulted in optimum conversion into its β -D-glucoside arbutin (2) within 10 h. A further metabolite, the primeveroside (3) of hydroquinone, appeared as a trace compound after 10 h. The formation of this diglycoside can be increased by further…