Search results for "methods"

showing 10 items of 4526 documents

Connecting Solar Orbiter remote-sensing observations and Parker Solar Probe in situ measurements with a numerical MHD reconstruction of the Parker sp…

2022

As a key feature, NASA's Parker Solar Probe (PSP) and ESA-NASA's Solar Orbiter (SO) missions cooperate to trace solar wind and transients from their sources on the Sun to the inner interplanetary space. The goal of this work is to accurately reconstruct the interplanetary Parker spiral and the connection between coronal features observed remotely by the Metis coronagraph on-board SO and those detected in situ by PSP at the time of the first PSP-SO quadrature of January 2021. We use the Reverse In-situ and MHD Approach (RIMAP), a hybrid analytical-numerical method performing data-driven reconstructions of the Parker spiral. RIMAP solves the MHD equations on the equatorial plane with the PLUT…

Plasma Physics (physics.plasm-ph)Settore FIS/05 - Astronomia E AstrofisicaAstrophysics - Solar and Stellar AstrophysicsPhysics - Space PhysicsSpace and Planetary ScienceFOS: Physical sciencesAstronomy and Astrophysicsmagnetohydrodynamics (MHD) – methods: numerical – solar wind – Sun: heliosphere – Sun: coronamagnetohydrodynamics(MHD)methods:numericalsolar windSun:heliosphereSun:coronaPhysics - Plasma PhysicsSolar and Stellar Astrophysics (astro-ph.SR)Space Physics (physics.space-ph)
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Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation.

1983

A rapid and easy final purification method is described for the isolation of plasmids from B. fragilis. Using RbTCA density gradient centrifugation in an airfuge ultracentrifuge ccc plasmid DNA can be separated from RNA, residual chromosomal DNA, linear and oc plasmid DNA. Pure ccc plasmid DNA is obtained from cultures of between 1 ml and 2 l in less than one day.

Plasmid preparationDifferential centrifugationDNA BacterialRNAchemistry.chemical_elementBiologyIsolation (microbiology)RubidiumMolecular biologyRubidiumBacteroides fragilisPlasmidchemistryGeneticsCentrifugation Density GradientUltracentrifugePurification methodsTrichloroacetic AcidMolecular BiologyPlasmidsMoleculargeneral genetics : MGG
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Platelet function testing in pigs using the Multiplate® Analyzer.

2019

PLOS ONE 14(8), e0222010 (2019). doi:10.1371/journal.pone.0222010

Platelet AggregationSwinePhysiology030204 cardiovascular system & hematologyPlatelet inhibitionPharmacologyAntiplatelet Therapy0302 clinical medicineOral administrationAnimal CellsPig ModelsMedicine and Health SciencesMedicinePlateletOral AdministrationRoutes of AdministrationMammalsMultidisciplinaryPharmaceuticsQREukaryotaHematologyAnimal ModelsClopidogrelBody FluidsBloodExperimental Organism SystemsVertebratesMedicineAnatomyCellular Typesmedicine.drugResearch ArticleBlood PlateletsPlateletsPlatelet Function TestsScience610Research and Analysis Methods03 medical and health sciencesAnimal modelDrug TherapyAnimalsddc:610Platelet activationBlood CoagulationApplication methodsPharmacologyBlood CellsAspirinbusiness.industryOrganismsBiology and Life Sciences030208 emergency & critical care medicineCell BiologyPlatelet ActivationReference valuesAmniotesPurinergic P2Y Receptor AntagonistsAnimal StudiesbusinessPlatelet Aggregation InhibitorsPloS one
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Subliminal fear priming potentiates negative facial reactions to food pictures in women with anorexia nervosa.

2010

BackgroundTo investigate hedonic reactivity and the influence of unconscious emotional processes on the low sensitivity to positive reinforcement of food in anorexia nervosa (AN).MethodAN and healthy women were exposed to palatable food pictures just after a subliminal exposure to facial expressions (happy, disgust, fear and neutral faces), either while fasting or after a standardized meal (hungerversussatiety). Both implicit [facial electromyographic (EMG) activity from zygomatic and corrugator muscles, skin conductance, heart rate, and videotaped facial behavior] and explicit (self-reported pleasure and desire) measures of affective processes were recorded.ResultsIn contrast to healthy wo…

PleasureMESH : Galvanic Skin ResponseAnorexia NervosaMESH : Recognition (Psychology)AudiologyDevelopmental psychologyAdult Affect Analysis of Variance Anorexia Nervosa/ psychology Cues Electromyography/methods/statistics & numerical data Face Facial Expression Fear/ psychology Female Food Galvanic Skin Response Heart Rate Humans Hunger Motivation MuscleMESH : FearTask Performance and AnalysisEmotional expressionMESH : Task Performance and AnalysisMESH : Muscle Skeletalmedia_commonMESH : Reinforcement (Psychology)MESH: Subliminal Stimulationdigestive oral and skin physiologyFearMESH: Recognition (Psychology)MESH: Reinforcement (Psychology)Facial ExpressionPsychiatry and Mental healthMESH: Photic StimulationMESH: PleasureMESH : MotivationVisual PerceptionMESH : FaceMESH : FoodFacial electromyographyReinforcement PsychologyMESH: Anorexia NervosaMESH : Visual Perceptionmedicine.medical_specialtymedia_common.quotation_subjectMESH: MotivationMESH : Facial ExpressionMESH: ElectromyographyMESH : HungerHumansMESH: HumansMESH: SatiationMESH: Visual PerceptionMESH : CuesElectromyographyMESH : HumansRecognition PsychologyMESH: AdultMESH: Task Performance and Analysismedicine.diseaseDisgustFoodFaceMESH: FemalePhotic StimulationMESH: CuesMESH: Facial ExpressionHunger[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionMESH: FearMESH : Photic StimulationMESH : Analysis of VarianceAnorexia nervosaHeart RateMESH: Unconscious (Psychology)MESH : FemaleMESH: Heart RateApplied PsychologyMESH: Muscle SkeletalUnconscious PsychologyMESH: AffectGalvanic Skin ResponseMESH : AdultSkeletal Photic Stimulation/ methods Pleasure Recognition (Psychology) Reinforcement (Psychology) Satiation Subliminal Stimulation Task Performance and Analysis Unconscious (Psychology) Visual PerceptionMESH : Unconscious (Psychology)MESH: HungerMESH: Galvanic Skin ResponseMESH : Anorexia NervosaMESH : ElectromyographyFemaleCuesPsychologyMESH : Subliminal StimulationPriming (psychology)MESH: FaceMESH: FoodAdultMESH : PleasureSatiationSubliminal StimulationPleasureMESH: Analysis of VariancemedicineMuscle SkeletalFacial expressionAnalysis of VarianceMotivationMESH : Heart RateSubliminal stimuliMESH : AffectAffect[SDV.AEN]Life Sciences [q-bio]/Food and NutritionMESH : Satiation
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TRIC: an automated alignment strategy for reproducible protein quantification in targeted proteomics

2016

Nature Methods, 13 (9)

Pluripotent Stem CellsProteomics0301 basic medicineAnalyteStreptococcus pyogenesSoftware toolQuantitative proteomicsProteomic analysisComputational biologyBiologyProteome informaticsProteomicsBioinformaticsBiochemistryArticleMass Spectrometry03 medical and health sciencesSequence Analysis ProteinProtein methodsHumansProtein PrecursorsHuman Induced Pluripotent Stem CellsMolecular BiologyElectronic Data ProcessingReproducibility of ResultsCell BiologyMass spectrometricTargeted proteomics030104 developmental biologyProteolysissense organsPeptidesSequence AlignmentAlgorithmsSoftwareBiotechnologyNature Methods
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2021

Abstract This paper reports on a teaching innovation using participant-generated drawings. Experienced managers were asked to produce a drawing to illustrate their work from an accounting perspective. The drawings were then used to make the managerial context of the participants the explicit starting point for personalized executive learning. This study is the first in the sphere of accounting education and research to take drawing seriously as a learning method. The results of the experiment show how drawing can be further used as a tool in management education by facilitating the visualization of the managerial contexts participants work within.

Point (typography)business.industry05 social sciencesPerspective (graphical)050301 educationContext (language use)Accounting050201 accountingPersonalized learningAccounting educationEducationVisualizationWork (electrical)Accounting0502 economics and businessLearning methodsbusinessPsychology0503 educationJournal of Accounting Education
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Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing st…

2021

Viruses are responsible for multiple infections in humans that impose huge health burdens on individuals and populations worldwide. Therefore, numerous diagnostic methods and strategies have been developed for prevention, management, and decreasing the burden of viral diseases, each having its advantages and limitations. Viral infections are commonly detected using serological and nucleic acid-based methods. However, these conventional and clinical approaches have some limitations that can be resolved by implementing other detector devices. Therefore, the search for sensitive, selective, portable, and costless approaches as efficient alternative clinical methods for point of care testing (P…

Point of care testingViral infectionsDiagnostic methodsSARS-CoV-2Computer sciencePoint-of-care testingSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)HIVArticleInfluenzaClinical methodAnalytical ChemistryMultiple infectionsVirus detectionDengueZikaRisk analysis (engineering)HBVMedical diagnosisSpectroscopyVirus classificationLateral flow assaysTrAC Trends in Analytical Chemistry
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Constraining the neutrino emission of gravitationally lensed Flat-Spectrum Radio Quasars with ANTARES data

2014

This paper proposes to exploit gravitational lensing effects to improve the sensitivity of neutrino telescopes to the intrinsic neutrino emission of distant blazar populations. This strategy is illustrated with a search for cosmic neutrinos in the direction of four distant and gravitationally lensed Flat-Spectrum Radio Quasars. The magnification factor is estimated for each system assuming a singular isothermal profile for the lens. Based on data collected from 2007 to 2012 by the ANTARES neutrino telescope, the strongest constraint is obtained from the lensed quasar B0218+357, providing a limit on the total neutrino luminosity of this source of 1.08×10^46 erg s-1. This limit is about one o…

Point sourceAstronomyAstrophysics::High Energy Astrophysical Phenomenagravitational lensingFOS: Physical sciencesgravitational lensing; neutrino astronomyAstrophysicsAstrophysics::Cosmology and Extragalactic Astrophysics01 natural sciencesLuminosityneutrino astronomyNeutrino0103 physical sciencesSensitivity (control systems)Blazar010303 astronomy & astrophysicsPhysicsHigh Energy Astrophysical Phenomena (astro-ph.HE)gravitational lensing; neutrino astronomy; Astronomy and AstrophysicsCOSMIC cancer database010308 nuclear & particles physicshigh energy astrophysical neutrinosAstrophysics::Instrumentation and Methods for AstrophysicsFísicaQuasarAstronomy and AstrophysicsGravitational lensFISICA APLICADANeutrinoMATEMATICA APLICADAAstrophysics - High Energy Astrophysical Phenomena[PHYS.ASTR]Physics [physics]/Astrophysics [astro-ph]
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Implantable collamer lens and femtosecond laser for myopia: comparison using an adaptive optics visual simulator.

2014

Purpose: To compare optical and visual quality of implantable collamer lens (ICL) implantation and femtosecond laser in situ keratomileusis (F-LASIK) for myopia. Methods: The CRX1 adaptive optics visual simulator (Imagine Eyes, Orsay, France) was used to simulate the wavefront aberration pattern after the two surgical procedures for -3-diopter (D) and -6-D myopia. Visual acuity at different contrasts and contrast sensitivities at 10, 20, and 25 cycles/degree (cpd) were measured for 3-mm and 5-mm pupils. The modulation transfer function (MTF) and point spread function (PSF) were calculated for 5-mm pupils. Results: F-LASIK MTF was worse than ICL MTF, which was close to diffraction-limited MT…

Point spread functionAdultLaser de excimerPhakic Intraocular LensesVisual acuityVisual acuitymedicine.medical_treatmentKeratomileusis Laser In SituLentes intraoculares; Sensibilidade de contrasteVisual AcuityCeratomileuse assistida por excimer laser in situ/métodosKeratomileusisPhakic intraocular lensMyopia/surgeryPupilKeratomileusis laser in situ/methodsContrast SensitivityYoung Adultlcsh:OphthalmologyLens Implantation IntraocularOptical transfer functionmedicineMyopiaHumansComputer SimulationContrast sensitivityAdaptive opticsSimulationPhysicsLasers excimer/therapeutic useAcuidade visualAberrometryAccommodation OcularGeneral MedicineOphthalmologyTreatment Outcomelcsh:RE1-994Lenses intraocularMiopia/cirurgiaOptometrySpatial frequencymedicine.symptomArquivos brasileiros de oftalmologia
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The INTEGRAL IBIS/ISGRI System Point Spread Function and Source Location Accuracy

2003

The imager on board INTEGRAL (IBIS) presently provides the most detailed sky images ever obtained at energies above 30 keV. The telescope is based on a coded aperture imaging system which allows to obtain sky images in a large field of view 29deg x 29deg with an angular resolution of 12'. The System Point Spread Function of the telescope and its detailed characteristics are here described along with the specific analysis algorithms used to derive the accurate point-like source locations. The derived location accuracy is studied using the first in-flight calibration data on strong sources for the IBIS/ISGRI system. The dependence of the calibrated location accuracy with the signal to noise r…

Point spread functionAstrophysics::High Energy Astrophysical PhenomenaData analysisFOS: Physical sciencesImage processingField of viewAstrophysicsAstrophysicsUNESCO::ASTRONOMÍA Y ASTROFÍSICAlaw.inventionTelescopeHigh angular resolutionSignal-to-noise ratioImage processinglawData analysis ; Image processing ; High angular resolutionAngular resolutionCoded aperture:ASTRONOMÍA Y ASTROFÍSICA::Astronomía óptica [UNESCO]Remote sensingIbisPhysicsbiologyUNESCO::ASTRONOMÍA Y ASTROFÍSICA::Astronomía ópticaAstrophysics (astro-ph)Astrophysics::Instrumentation and Methods for AstrophysicsAstronomy and Astrophysicsbiology.organism_classificationSpace and Planetary Science:ASTRONOMÍA Y ASTROFÍSICA [UNESCO]
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