Search results for "microarray."

showing 10 items of 384 documents

Microarray-Based Determination of Response of Tumor Cells to Cycloshikonin

2011

biologyMicroarraybusiness.industryTumor cellsTraditional Chinese medicinePharmacologyLithospermum erythrorhizonbiology.organism_classificationBiochemistryPharmacogenomicsGeneticsCancer researchMolecular MedicineMedicinebusinessBiotechnologyForum on Immunopathological Diseases and Therapeutics
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Proton-irradiated breast cells: molecular points of view

2019

Abstract Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and descri…

breast cancer cDNA microarray gene signature proton therapy radiation Breast Breast Neoplasms Cell Line Tumor DNA Complementary Dose-Response Relationship Radiation Female Gene Expression Profiling Gene Expression Regulation Neoplastic Humans Inflammation MCF-7 Cells Oligonucleotide Array Sequence Analysis Phenotype Proton Therapy Radiation Tolerance Radiotherapy ProtonsDNA ComplementaryHealth Toxicology and Mutagenesismedicine.medical_treatmentBreast NeoplasmsCell fate determinationRadiation Tolerancegene signature03 medical and health sciences0302 clinical medicineBreast cancerbreast cancerCell Line TumorRegular Papermedicineproton therapyHumansRadiology Nuclear Medicine and imagingBreastClonogenic assayBiologyProton therapyOligonucleotide Array Sequence Analysis030304 developmental biologyInflammationcDNA microarray0303 health sciencesRadiotherapyChemistryGene Expression ProfilingBreast cancer; radiation; cDNA microarray; gene signature; proton therapyCancerDose-Response Relationship RadiationGene signaturemedicine.diseaseGene Expression Regulation NeoplasticGene expression profilingRadiation therapyradiationPhenotype030220 oncology & carcinogenesisMCF-7 CellsCancer researchFemaleProtons
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Tissue microarray technology in breast cancer subtyping

2008

breast cancerrintasyöpätissue microarray technologyimmunohistochemistrybreast carcinomacancer marker
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Gene Expression Profile of Chronic Myeloid Leukemia Innately Resistant to Imatinib

2007

Background. Most chronic myeloid leukemia patients who receive imatinib as first line-terapy will obtain, after 12 months treatment, complete cytogenetic and molecular response . However several cases will not achieve molecular response, but their innate mechanism(s) of resistance remain poorly understood. We tried to explore the molecular events involved in innate resistance in CML. Study design. Five patients who were molecular “non responder” and seven “major” responder were investigated by using the expression profile of a set of 380 genes. Multiple testing procedure (MTP), Significance Analysis of Microarrays (SAM), Empirical Bayes Analysis of Microarrays (EBAM), False Discovery Rate (…

business.industryMyeloid leukemiaImatinibGeneral MedicineCell cycleMolecular ResponseImmunologySignificance analysis of microarraysCancer researchmedicineEpigeneticsDNA microarraybusinessGenemedicine.drugClinical Leukemia
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MLML2R

2019

R package for maximum likelihood estimation of DNA methylation and hydroxymethylation proportions.

carbohydrates (lipids)organic chemicalsbacteriaheterocyclic compoundsEpigeneticsMicroarray experimentMethylated DNA immunoprecipitation
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Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.

2012

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

chemistry.chemical_classificationChromatographytechnology industry and agricultureBiomedical EngineeringAssayBioprintingDrug Evaluation PreclinicalBioengineeringGeneral ChemistryMicroarray AnalysisBiochemistryMembraneEnzymechemistryLuminescent MeasurementsCytochrome P-450 CYP3ANanotechnologyBiochipBiosensorInkjet printingDrug metabolismLab on a chip
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Inside Cover: Antibody Induction Directed against the Tumor-Associated MUC4 Glycoprotein (ChemBioChem 6/2015)

2015

chemistry.chemical_classificationbiologyChemistryOrganic ChemistryCancermedicine.diseaseBiochemistryMolecular biologyGlycopeptideAntibody inductionbiology.proteinmedicineMolecular MedicineCover (algebra)AntibodyDNA microarrayGlycoproteinMolecular BiologyChemBioChem
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Computational cluster validation for microarray data analysis: experimental assessment of Clest, Consensus Clustering, Figure of Merit, Gap Statistic…

2008

Abstract Background Inferring cluster structure in microarray datasets is a fundamental task for the so-called -omic sciences. It is also a fundamental question in Statistics, Data Analysis and Classification, in particular with regard to the prediction of the number of clusters in a dataset, usually established via internal validation measures. Despite the wealth of internal measures available in the literature, new ones have been recently proposed, some of them specifically for microarray data. Results We consider five such measures: Clest, Consensus (Consensus Clustering), FOM (Figure of Merit), Gap (Gap Statistics) and ME (Model Explorer), in addition to the classic WCSS (Within Cluster…

clustering microarray dataMicroarrayComputer scienceStatistics as Topiccomputer.software_genrelcsh:Computer applications to medicine. Medical informaticsBiochemistryStructural BiologyDatabases GeneticConsensus clusteringStatisticsCluster (physics)AnimalsCluster AnalysisHumansCluster analysislcsh:QH301-705.5Molecular BiologyOligonucleotide Array Sequence AnalysisStructure (mathematical logic)Microarray analysis techniquesApplied MathematicsComputational BiologyComputer Science ApplicationsBenchmarkingComputingMethodologies_PATTERNRECOGNITIONlcsh:Biology (General)Gene chip analysislcsh:R858-859.7Data miningDNA microarraycomputerAlgorithmsSoftwareResearch ArticleBMC Bioinformatics
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Cortical gene expression in spinal cord injury and repair: insight into the functional complexity of the neural regeneration program

2011

Traumatic spinal cord injury (SCI) results in the formation of a fibrous scar acting as a growth barrier for regenerating axons at the lesion site. We have previously shown (Klapka et al., 2005) that transient suppression of the inhibitory lesion scar in rat spinal cord leads to long distance axon regeneration, retrograde rescue of axotomized cortical motoneurons, and improvement of locomotor function. Here we applied a systemic approach to investigate for the first time specific and dynamic alterations in the cortical gene expression profile following both thoracic SCI and regeneration-promoting anti-scarring treatment (AST). In order to monitor cortical gene expression we carried out micr…

corticospinal tractmedicine.medical_treatmentlesion scarlcsh:RC321-571Cellular and Molecular Neuroscienceanti-scarring treatmentmedicineAxonlcsh:Neurosciences. Biological psychiatry. Neuropsychiatrysensorimotor cortexMolecular BiologySpinal cord injurySpinal Cord RegenerationOriginal Researchbusiness.industryRegeneration (biology)axonal regenerationmedicine.diseaseSpinal cordspinal cord injuryaxotomymedicine.anatomical_structureCorticospinal tractAxotomybusinessmicroarrayNeural developmentNeuroscienceNeuroscienceFrontiers in Molecular Neuroscience
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Cancer Signaling Transcriptome Is Upregulated in Type 2 Diabetes Mellitus

2020

We aimed to explore the differences in the whole transcriptome of peripheral blood mononuclear cells between elderly individuals with and without type 2 diabetes (T2D). We conducted a microarray-based transcriptome analysis of 19 individuals with T2D and 15 without. Differentially expressed genes according to linear models were submitted to the Ingenuity Pathway Analysis system to conduct a functional enrichment analysis. We established that diseases, biological functions, and canonical signaling pathways were significantly associated with T2D patients when their logarithms of Benjamini&ndash

endocrine system diseasesMicroarrayIntegrinT cellslcsh:Medicine030209 endocrinology & metabolismInflammationPeripheral blood mononuclear cellArticleDiabetis no-insulinodependentTranscriptome03 medical and health scienceschemistry.chemical_compound0302 clinical medicineDownregulation and upregulationmedicinecancerNon-insulin-dependent diabetesCàncer030304 developmental biologyCancer0303 health sciencesbiologybusiness.industrylcsh:Rnutritional and metabolic diseasesGeneral Medicinesignaling pathwayschemistryCèl·lules TGuanosine diphosphateCancer researchbiology.proteintype 2 diabetesmedicine.symptomSignal transductionbusinesstranscriptomemicroarray
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