6533b822fe1ef96bd127d748

RESEARCH PRODUCT

Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.

Sabrina CastellanoBruno PignataroClelia GalatiGiuseppe Arrabito

subject

chemistry.chemical_classificationChromatographytechnology industry and agricultureBiomedical EngineeringAssayBioprintingDrug Evaluation PreclinicalBioengineeringGeneral ChemistryMicroarray AnalysisBiochemistryMembraneEnzymechemistryLuminescent MeasurementsCytochrome P-450 CYP3ANanotechnologyBiochipBiosensorInkjet printingDrug metabolism

description

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

yearjournalcountryeditionlanguage
2012-11-08Lab on a chip
10.1039/c2lc40948hhttps://pubmed.ncbi.nlm.nih.gov/23132304