Search results for "microbial"
showing 10 items of 2041 documents
Extraction of bioactive compounds and essential oils from mediterranean herbs by conventional and green innovative techniques: A review
2018
International audience; Market interest in aromatic plants from the Mediterranean is continuously growing mainly due to their medicinal and bioactive compounds (BACs) with other valuable constituents from essential oils (EOs). From ancient times, these plants have been important condiments for traditional Mediterranean cuisine and remedies in folk medicine. Nowadays, they are considered as important factors for food quality and safety, due to prevention of various deteriorative factors like oxidations and microbial spoilage. EOs have different therapeutic benefits (e.g. antioxidant, anti-inflammatory, antimicrobial, and antifungal), while BACs mostly affect nutritive, chemical, microbiologi…
Benthic microbial indicators of fish farm impact in a coastal area of the Tyrrhenian Sea
2004
Abstract We studied the impact of organic loads due to the biodeposition of a fish farm in a non-impacted coastal area of the Tyrrhenian Sea (Western Mediterranean). Sediment chemistry and benthic microbial community were investigated from July 1997 to February 1998 on monthly basis at two stations: one was located under the fish farm, while the second was about 1 km away, and served as a reference site. The presence of the cage induced rapid changes in the benthic conditions: the sediments were rapidly (after 6 weeks) reduced. A significant accumulation of biopolymeric carbon was observed beneath the cage both 2 weeks after the initial cage deployment, on non-impacted sediments, and 5-7 mo…
SOIL QUALITY INDICATORS AS AFFECTED BY SHALLOW TILLAGE IN A VINEYARD GROWN IN A SEMIARID MEDITERRANEAN ENVIRONMENT
2016
Within the Mediterranean basin, soil tillage enhances the mineralisation of soil organic matter. We assessed the short-term impact of shallow tillage [field cultivator (FC), rotary tiller (RT) and spading machine (SM)] on some soil quality indicators [bulk density, water-stable aggregates, total and labile organic C pools (microbial biomass and extractable organic C), soil respiration and related eco-physiological indexes] in a Sicilian vineyard. Also no tillage was included. We hypothesized that (i) RT and FC worsened soil quality indicators more than SM, and (ii) within the same tillage system, labile C pools, soil respiration and eco-physiological indexes will respond more efficiently th…
Occurrence of Edwardsiella tarda in wild European eels Anguilla anguilla from Mediterranean Spain
2007
Pure cultures of Edwarsiella tarda were isolated from body ulcers and internal organs of wild European eels caught in a Mediterranean freshwater coastal lagoon (Albufera Lake, Valencia, Spain) over a 1 yr period. Overall, the E. tarda isolation rate from wild eels was 9%, but this increased to 22.8% in diseased individuals. All 22 E. tarda isolates belonged to the 'wild-type' biogroup of the species and were virulent for eels (lethal dose that kills 50% of exposed individuals [LD50 dose]: 10(4.85) to 10(6.83) CFU ind.(-1)), and therefore represented the aetiological agent of the haemorrhagic disease observed in wild European eels. The E. tarda isolates and E. tarda CECT 894T type strain wer…
Individual variability of mytimycin gene expression in mussel
2012
The antifungal peptide mytimycin (MytM) is synthesized by hemocytes of the Mediterranean mussel, Mytilus galloprovincialis. In addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual M. galloprovincialis, before and after challenge. Within untreated mussel, MytM mRNA was observed by ISH in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. Injection with Fusarium oxysporum increased such percentage, but in only some of the mussels. Similarly, MytM gene expression increased after injection in only some of the mussels, as measured by qPCR. Responders and not…
(19)F NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
2014
AbstractMany amphiphilic antimicrobial peptides permeabilize bacterial membranes via successive steps of binding, re-alignment and/or oligomerization. Here, we have systematically compared the lipid interactions of two structurally unrelated peptides: the cyclic β-pleated gramicidin S (GS), and the α-helical PGLa. 19F NMR was used to screen their molecular alignment in various model membranes over a wide range of temperatures. Both peptides were found to respond to the phase state and composition of these different samples in a similar way. In phosphatidylcholines, both peptides first bind to the bilayer surface. Above a certain threshold concentration they can re-align and immerse more dee…
Performance of industrial scale hollow-fibre membranes in a submerged anaerobic MBR (HF-SAnMBR) system at mesophilic and psychrophilic conditions
2013
The aim of this work was to evaluate the effect of temperature on the performance of industrial hollow-fibre (HF) membranes treating urban wastewater in a submerged anaerobic MBR system (SAnMBR). To this end, a demonstration plant with two commercial HF ultrafiltration membrane modules (PURON®, Koch Membrane Systems, PUR-PSH31) was operated at 20, 25 and 33 °C. The mixed liquor total solid (MLTS) level was a key factor affecting membrane permeability (K). K was higher under psychrophilic than mesophilic conditions when operating at similar transmembrane fluxes and MLTS, because the biomass activity of the psychrophilic mixed liquor was lower than the mesophilic mixed liquor. Thus, lower ext…
Impact of Gluten-Friendly Bread on the Metabolism and Function of In Vitro Gut Microbiota in Healthy Human and Coeliac Subjects
2016
The main aim of this paper was to assess the in vitro response of healthy and coeliac human faecal microbiota to gluten-friendly bread (GFB). Thus, GFB and control bread (CB) were fermented with faecal microbiota in pH-controlled batch cultures. The effects on the major groups of microbiota were monitored over 48 h incubations by fluorescence in situ hybridisation. Short-chain fatty acids (SCFAs) were measured by high-performance liquid chromatography (HPLC). Furthermore, the death kinetics of Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, Staphylococcus aureus, and Salmonella Typhimurium in a saline solution supplemented with GFB or CB were also assessed. The experiment…
Influence of cell-cell contact between L. thermotolerans and S. cerevisiae on yeast interactions and the exo-metabolome
2019
International audience; Sequential fermentation of grape must inoculated with L. thermotolerans and then S. cerevisiae 24 h later (typical wine-making practice) was conducted with or without cell-cell contact between the two yeast species. We monitored cell viability of the two species throughout fermentation by flow cytometry. The cell viability of S. cerevisiae decreased under both conditions, but the decrease was greater if there was cell-cell contact. An investigation of the nature of the interactions showed competition between the two species for nitrogen compounds, oxygen, and must sterols. Volatile-compound analysis showed differences between sequential and pure fermentation and that…
Bioactive metabolites from the endophytic fungus Ampelomyces sp. isolated from the medicinal plant Urospermum picroides
2007
Extracts of cultures grown in liquid or on solid rice media of the fungal endophyte Ampelomyces sp. isolated from the medicinal plant Urospermum picroides exhibited considerable cytotoxic activity when tested in vitro against L5178Y cells. Chromatographic separation yielded 14 natural products that were unequivocally identified based on their 1H and 13C NMR as well as mass spectra and comparison with previously published data. Six compounds (2, 4, 5, 7, 9 and 11) were natural products. Both fungal extracts differed considerably in their secondary metabolites. The extract obtained from liquid cultures afforded a pyrone (2) and sulfated anthraquinones (7 and 9) along with the known compounds …