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RESEARCH PRODUCT
Individual variability of mytimycin gene expression in mussel
Mylène ToubianaFranck CantetPhilippe RochMatteo CammarataMaria Giovanna ParisiMolruedee Sonthisubject
Mediterranean musselHemocytesanimal structuresPopulationAquatic ScienceReal-Time Polymerase Chain ReactionMicrobiologyFusariumFusarium oxysporumGene expressionAnimalsEnvironmental ChemistryRNA MessengereducationGeneIn Situ HybridizationMytilusMessenger RNAeducation.field_of_studybiologyGene Expression ProfilingfungiGeneral MedicineMusselbiology.organism_classificationMytilusGene Expression RegulationAntifungal Antimicrobial Gene expression Challenge Innate immunityAntimicrobial Cationic Peptidesdescription
The antifungal peptide mytimycin (MytM) is synthesized by hemocytes of the Mediterranean mussel, Mytilus galloprovincialis. In addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual M. galloprovincialis, before and after challenge. Within untreated mussel, MytM mRNA was observed by ISH in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. Injection with Fusarium oxysporum increased such percentage, but in only some of the mussels. Similarly, MytM gene expression increased after injection in only some of the mussels, as measured by qPCR. Responders and not responders are common evidence in any given population of organisms. Nevertheless, even if the use of proper pool size selection has been practised to find out and evaluate the most common response trends, individual analyses must be regarded as optimal.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-09-01 | Fish & Shellfish Immunology |