Search results for "microbiologia"

Drug resistance patterns of bacteria isolated from patients with nosocomial pneumonia at Tehran hospitals during 2009-2011

2013

Introduction: Nosocomial pneumonia remains an important cause of mortality and morbidity worldwide. Surveillance programs play an important role in the identification of common etiologic agents and local patterns of antimicrobial resistance. Methodology: In this study we determined the frequency and antimicrobial susceptibility of pathogens isolated from patients with nosocomial pneumonia during 2009 to 2011. Results: A total of 642 bacteria were isolated from 516 suspected samples. Acinetobacter baumannii (21.1%, n = 136), was the commonest isolated pathogen followed by Pseudomonas aeruginosa (17.4%, n = 112) , Staphylococcus aureus (15.8%, n = 102) and enterococci (8.4% n = 54). The most …

Innovative ready to use carrier-bacteria devices for bioremediation of oil contaminated water

2018

Bioremediation, that uses microorganisms to remove environmental pollutants, is the best way of restoring the environment due to its low cost and sustainability. Immobilization of microorganisms capable of degrading specific contaminants significantly promotes bioremediation processes. An innovative ready to use bioremediation system to clean up oil-contaminated water was developed immobilizing highly performant marine and soil HC degrading bacteria, on biodegradable oil-absorbing carriers. Two soil Actinobacteria (Gordonia sp. SoCg, Nocardia sp. SoB) and two marine Gammaproteobacteria (Alcanivorax sp. SK2, Oleibacter sp.5), were immobilized on biopolymeric membranes prepared by electrospin…

Regulation of antibiotic biosynthesis in Actinomycetes.

2013

Enhancement of antibiotic productions by plasma modified PLA electrospun membranes in Streptomyces coelicolor immobilized-cell cultivations

2017

This study, encouraging the use of PLA membranes for actinomycetes cultivations, could unveil functional insights associated with antibiotic production and S. coelicolor cellimmobilization

OXA-163-Producing Klebsiella pneumoniae in Cairo, Egypt, in 2009 and 2010

2012

ABSTRACT Two genetically unrelated OXA-163-carrying Klebsiella pneumoniae strains were identified from two infection cases in June 2009 and May 2010 in Cairo, Egypt. OXA-163-producing Enterobacteriaceae had been previously reported in Argentina only. Both patients had no history of travel abroad. The emergence of this newly recognized OXA-48-related β-lactamase able to hydrolyze cephalosporins and carbapenems is especially worrying in a geographic area where OXA-48 is endemic and effective surveillance for antibiotic resistance is largely unaffordable.

Persistent Endemicity of Salmonella bongori 48:z 35 :− in Southern Italy: Molecular Characterization of Human, Animal, and Environmental Isolates

2002

ABSTRACT From 1984 to 1999, we collected 31 isolates of the rare serovar Salmonella bongori 48:z 35 :− in southern Italy. Twenty-four of the isolates were from cases of acute enteritis in humans. Pulsed-field gel electrophoresis analysis showed that all but one of our isolates were at least 80% similar. Our findings suggest that genetically related S. bongori 48:z 35 :− strains are endemically circulating in southern Italy.

Characterization of measles virus strains circulating in Southern Italy (Palermo area, Sicily) between 2010 and 2011

2015

Measles virus (MV) was classified in 24 genotypes that show a distinct geographic distribution. Genotypes contain multiple distinct lineages. In 2011 large outbreaks of measles occurred in Italy and in many European countries. Aims of this study are to analyze the intra-genotype variability and to follow the importation and the spread of new MV strains in Sicily. A fragment of 450. bps of MV C-terminal nucleoprotein was sequenced from sera of 73 Sicilian patients with symptomatic measles infections, occurred between 2010 and 2011. Five MV strains were D4 genotype and 68 were D8 genotype. The MV/D4 sequences were related to MV/D4-Enfield variant. Two lineages of MV/D8 genotypes, related to M…

HPV infection in semen: results from a new molecular approach

2019

AbstractHuman papillomavirus (HPV) is the agent of the most common sexually transmitted diseases causing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPV infection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developed a new approach to evaluate virus localisation in the different semen components. We analysed also the specific genotype localisation and viral DNA quantity by qPCR. Results show that HPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HP…

Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients

2020

Abstract Purpose To evaluate the correlation between the DNA Fragmentation Index (DFI) and sperm morphology in patients undergoing ICSI, as a predictive parameter in reproductive outcomes. Methods A retrospective study was conducted on 125 infertile patients enrolled in a fertility clinic. Seminal characteristics were measured following the WHO guidelines (2010) for the examination of the seminal fluid. After collecting motile sperm population by pellet swim up, DFI was calculated and simultaneously associated with sperm morphology using in situ TUNEL assay and an image analyzer software in at least 250 spermatozoa for each patient. Results All subjects were divided into two groups accordin…

The epidemiology of Varicella Zoster Virus infection in Italy

2008

Abstract Background The epidemiological importance of varicella and zoster and the availability of an efficacious and safe vaccine have led to an important international debate regarding the suitability of mass vaccination. The objective of the study was to describe the epidemiology of varicella and zoster in Italy and to determine whether there have been changes with respect to observations provided by an analogous study conducted 8 years ago, in order to define the most appropriate vaccination strategy. Methods A number of data sources were evaluated, a cross-sectional population-based seroprevalence study was conducted on samples collected in 2004, and the results were compared with data…