Search results for "microbiology"

Devices containing allyl isothiocyanate against the growth of spoilage and mycotoxigenic fungi in mozzarella cheese

2018

Filter paper and stickers containing allyl isothiocyanate (AIT), as well as pouches containing mustard meal and water for intrinsic production of AIT, were tested against aflatoxigenic strains of Penicillium digitatum CECT 2954 and Aspergillus parasiticus CECT 2681 in sliced mozzarella. Slices were inoculated with either fungus, packaged in plastic bags or plastic trays with one antimicrobial device (2–16 µL/L of AIT). Control groups presented visual growth of P. digitatum after 19.0 ± 1.5 d in bags and 26.1 ± 2.1 d in trays. A. parasiticus was visually detectable after 41.4 ± 1.7 d and 28.4 ± 1.5 d in trays and bags, respectively. AIT‐treated samples did not present visual fungal growth fo…

Microbial Colonization of the Peri-Implant Sulcus and Implant Connection of Implants Restored With Cemented Versus Screw-Retained Superstructures: A …

2016

The aim of this study is to investigate peri-implant and intraconnection microflora of healthy implants restored with cemented and screwed superstructures.Patients with two to three implants restored with cemented or screwed restorations and 5 years of follow-up were recruited. Samples were taken from peri-implant sulci, adjacent teeth, and the inner portion of connections. Prevalence of positive sites and bacterial loads for 10 microorganisms were obtained with quantitative real-time polymerase chain reaction. Implant connection permeability to the studied microorganisms was estimated using a standardized bacterial contamination index. Statistical analysis was performed using a generalized…

Characterization of a Novel Conformational GII.4 Norovirus Epitope: Implications for Norovirus-Host Interactions

2016

ABSTRACT Human noroviruses (NoVs) are the main etiological agents of acute gastroenteritis worldwide. While NoVs are highly diverse (more than 30 genotypes have been detected in humans), during the last 40 years most outbreaks and epidemics have been caused by GII.4 genotype strains, raising questions about their persistence in the population. Among other potential explanations, immune evasion is considered to be a main driver of their success. In order to study antibody recognition and evasion in detail, we analyzed a conformational epitope recognized by a monoclonal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface plasmon resonance. Our results show that the predi…

Un accompagnement sur mesure

2018

Resume Le diagnostic, l’initiation de traitement, les switches therapeutiques et les periodes a risques sont des moments cles dans l’accompagnement du patient atteint de sclerose en plaques. Tout au long de sa prise en charge, le malade exprime de nombreuses attentes vis-a-vis du pharmacien, ce qui implique une collaboration renforcee entre les professionnels de sante et, pour l’officinal, le developpement de nouveaux champs d’intervention.

Délivrance d’une ordonnance dans le cadre d’un trouble anxieux

2020

Afin d’etre correctement dispensee par l’equipe officinale, puis prise en charge par la caisse d’Assurance maladie, une prescription medicale de medicaments doit contenir un certain nombre d’elements obligatoires. Les erreurs reglementaires qui ont ete introduites sur l’ordonnance d’une patiente presentant des troubles anxieux doivent etre identifiees.

Conseil en aromathérapie et sommeil

2019

Resume Que la plainte concerne un endormissement difficile, des eveils nocturnes ou un reveil matinal precoce, l’aromatherapie peut constituer une aide interessante pour le patient insomniaque qui envisage une approche non medicamenteuse.

Bacterial Biofilm Inhibition in the Development of Effective Anti-Virulence Strategy

2018

There is an urgent need for new therapeutic strategies to counteract the global threat of antibiotic resistance, which has become, in recent years, one of the major public health concern. An important contribution to the microbial survival in hostile environments has been given by the capability of pathogens to form sessile communities able to adhere to biotic or abiotic surfaces, known as biofilms.

Les bronchospasmes iatrogènes

2016

Resume Les bronchospasmes sont des effets indesirables pouvant mettre en jeu le pronostic vital. Les symptomes ressemblent a ceux de la crise d’asthme aigue grave. Le pharmacien doit savoir reconnaitre ces signes, mais aussi envisager la cause iatrogene afin d’identifier le plus vite possible le traitement suspect et conseiller au mieux le patient sur la conduite a tenir.

In vitro study of antimicrobial activity on Klebsiella Pneumoniae biofilms in endotracheal tubes

2019

Effective treatment approaches for biofilms in endotracheal tubes (ETTs) are lacking. In this study, we evaluated the in vitro effects of five antimicrobials against biofilms formed by Klebsiella pneumoniae in ETTs. K. pneumoniae was added to minimal mucin medium prior to inoculation in microtiter plates containing ETT fragments. Biofilm susceptibility was assessed by crystal violet staining. At 24 h, the antimicrobials significantly reduced biofilm formation. At 48 h, all of the antimicrobial agents exhibited significant reductions in biofilm formation, even at concentrations above the minimum inhibitory concentration (MIC). Tigecycline and fosfomycin showed the greatest inhibition capacit…

2019

Abstract Type B Coxsackieviruses (CVBs) are a common cause of acute and chronic myocarditis, dilated cardiomyopathy and aseptic meningitis. However, no CVB-vaccines are available for human use. We have previously produced virus-like particles (VLPs) for CVB3 with a baculovirus-insect cell production system. Here we have explored the potential of a VLP-based vaccine targeting CVB1 and describe the production of CVB1-VLPs with a scalable VLP purification method. The developed purification method consisting of tangential flow filtration and ion exchange chromatography is compatible with industrial scale production. CVB1-VLP vaccine was treated with UV-C or formalin to study whether stability a…