Search results for "microbiota."

Análisis de procesos inmunorreguladores en helmintiasis intestinales mediante el modelo Echinostoma caproni-ratón

2021

En la presente Tesis Doctoral se analizan diferentes parámetros que intervienen en las interacciones parásito-hospedador en helmintiasis intestinales mediante el empleo del modelo experimental Echinostoma caproni-ratón ICR. Para ello, se realizan diferentes estudios en los que se analiza y compara la respuesta inmunitaria en infecciones primarias, asociadas a susceptibilidad, y secundarias, asociadas a resistencia. Los resultados obtenidos demuestran que la susceptibilidad del ratón a la infección primaria con E. caproni se debe a su incapacidad de responder con producción de IL-25 lo cual determina que se genere una respuesta Th1 asociada a susceptibilidad. Asimismo, estos resultados ponen…

Metabolomics: An Emerging Approach to Understand Pathogenesis and to Assess Diagnosis and Response to Treatment in Spondyloarthritis

2022

Spondyloarthritis (SpA) is a group of rheumatic diseases whose pathogenesis relies on a complex interplay between genetic and environmental factors. Over the last several years, the importance of the alteration of the gut microbiota, known as dysbiosis, and the interaction of bacterial products with host immunity have been highlighted as intriguing key players in SpA development. The recent advent of the so called “-omics” sciences, that include metabolomics, opened the way to a new approach to SpA through a deeper characterisation of the pathogenetic mechanisms behind the disease. In addition, metabolomics can reveal potential new biomarkers to diagnose and monitor SpA patients. The aim of…

Effect of aerobic exercise and low carbohydrate diet on pre-diabetic non-alcoholic fatty liver disease in postmenopausal women and middle aged men: s…

2014

Background. Pre-diabetes and non-alcoholic fatty liver disease (NAFLD) are associated with an unhealthy lifestyle and pose extremely high costs to the healthcare system. In this study, we aim to explore whether individualized aerobic exercise (AEx) and low carbohydrate diet (LCh) intervention affect hepatic fat content (HFC) in pre-diabetes via modification of gut microbiota composition and other post-interventional effects. Methods/design. A 6-month randomized intervention with 6-month follow-up is conducted from January 2013 to December 2015. The target sample size for intervention is 200 postmenopausal women and middle-aged men aged 50–65 year-old with pre-diabetes and NAFLD. The qualifi…

Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice.

2018

Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E. cloacae treatment groups. The E. cloacae treatment group received live bacterial cells in PBS intragastrically twice a week, every ot…

Il Microbiota delle piante, ovvero le piante hanno bisogno dei microrganismi

2017

Le relazioni tra le piante e i fattori ambientali (il clima, i nutrienti, l’acqua, la composizione del suolo, etc) sono da tempo ampiamente studiate. Resta invece ancora molto da capire sul ruolo che la componente biologica del suolo (ci sono oltre cento milioni di cellule microbiche in un grammo di suolo!) ha sullo sviluppo e la salute delle piante. Nella rizosfera soprattutto (la porzione di suolo influenzata dalla presenza di radici), gli essudati radicali favoriscono lo sviluppo di microrganismi (batteri, funghi, protozoi, virus) che interagiscono con le radici e con il suolo nell’influenzare i processi di trasformazione ed assorbimento dei nutrienti, la crescita della pianta e le infez…

Isolation and characterization of sea urchin P. lividus microbiota from coelomic fluid

2015

Role of Biological Sex in the Cardiovascular-Gut Microbiome Axis

2022

There has been a recent, unprecedented interest in the role of gut microbiota in host health and disease. Technological advances have dramatically expanded our knowledge of the gut microbiome. Increasing evidence has indicated a strong link between gut microbiota and the development of cardiovascular diseases (CVD). In the present article, we discuss the contribution of gut microbiota in the development and progression of CVD. We further discuss how the gut microbiome may differ between the sexes and how it may be influenced by sex hormones. We put forward that regulation of microbial composition and function by sex might lead to sex-biased disease susceptibility, thereby offering a mechani…

Land use effects on bacterial diversity in Mediterranean soils vulnerable to desertification

2021

The soil microbiota is recognized as key player in both diversity and productivity of terrestrial ecosystems and it is mandatory to include its role in soil quality evaluation. The bacterial communities in soils vulnerable to desertification can undergo perturbations by various human activities, such as unsustainable land use and management, and by climate change. Alteration in microbial diversity and composition are sentinels of the impact of environmental changes on soil quality. Within the LIFE Desert-Adapt project we analyze the soil bacterial diversity and composition across European Soils threatened by desertification in the southern Mediterranean regions (Italy, Spain and Portugal) a…

An Integrated Approach to Skeletal Muscle Health in Aging

2023

A decline in muscle mass and function represents one of the most problematic changes associated with aging, and has dramatic effects on autonomy and quality of life. Several factors contribute to the inexorable process of sarcopenia, such as mitochondrial and autophagy dysfunction, and the lack of regeneration capacity of satellite cells. The physiologic decline in muscle mass and in motoneuron functionality associated with aging is exacerbated by the sedentary lifestyle that accompanies elderly people. Regular physical activity is beneficial to most people, but the elderly need well-designed and carefully administered training programs that improve muscle mass and, consequently, both funct…

Six-Week Endurance Exercise Alters Gut Metagenome That Is not Reflected in Systemic Metabolism in Over-weight Women.

2018

Recent studies suggest that exercise alters the gut microbiome. We determined whether six-weeks endurance exercise, without changing diet, affected the gut metagenome and systemic metabolites of overweight women. Previously sedentary overweight women (n = 19) underwent a six-weeks endurance exercise intervention, but two were excluded due to antibiotic therapy. The gut microbiota composition and functions were analyzed by 16S rRNA gene amplicon sequencing and metagenomics. Body composition was analyzed with DXA X-ray densitometer and serum metabolomics with NMR metabolomics. Total energy and energy-yielding nutrient intakes were analyzed from food records using Micro-Nutrica software. Serum…