Search results for "milieu"
showing 10 items of 19813 documents
Incertitude et présentification en hypnose clinique
2018
Nous proposons d’analyser une situation clinique dans une perspective de psychothérapie intégrative. Nous nous inscrivons dans le cadre de différentes approches : humaniste, psychodynamique, cognitive et comportementale, hypnose et EMDR. Dans ce contexte, nous présentons le parcours d’une patiente dont les réactions ont nécessité des aménagements du cadre thérapeutique et un changement de référentiel théorique. Nous sommes passés avec Mme Barnes d’un travail centré sur les cognitions et les comportements à une approche sensorielle et expérientielle par hypnose. La thérapie a initialement ciblé une symptomatologie anxieuse pour finalement se développer sur un versant existentiel intégrant la…
Phospholipase activities associated with the tonoplast from Acer pseudoplatanus cells: identification of a phospholipase A1 activity
1995
In higher plants, the lipolytic enzymes and their physiological functions are not well characterized [1]. Most reports demonstrated that phospholipid catabolism in plants is achieved by the concerted actions of membrane-bound enzymes including phospholipase D, phosphatidate phosphatase, lipolytic acyl hydrolases and lipoxygenases [1,2]. With the exception of the phospholipase D, the literature on plant phospholipases is still very limited. We previously reported that tonoplast from Acer pseudoplatanus cells contains small amounts of phosphatidc acid and lysophospholipids, which were produced together with free fatty acids, particularly after addition of Ca2+[3]. These data suggested the pos…
Intelligence artificielle : quel avenir en anatomie pathologique ?
2019
Resume Les techniques d’intelligence artificielle et en particulier les reseaux de neurones profonds (Deep Learning) sont en pleine emergence dans le domaine biomedical. Les reseaux de neurones s’inspirent du modele biologique, ils sont interconnectes entre eux et suivent des modeles mathematiques. Lors de l’utilisation des reseaux de neurones artificiels, deux phases sont necessaires : une phase d’apprentissage et une phase d’exploitation. Les deux principales applications sont la classification et la regression. Des outils informatiques comme les processeurs graphiques accelerateurs de calcul ou des bibliotheques de developpement specifiques ont donne un nouveau souffle a ces techniques. …
Reconstruction of Past Dynamics of Methane-Oxidizing Bacteria in Lake Sediments Using a Quantitative PCR Method: Connecting Past Environmental Change…
2019
AbstractIn this study, a quantitative PCR (qPCR) method was applied to amplify ancient DNA (aDNA) of different methane-oxidizing bacteria (MOB) types in lake sediments and to reconstruct microbial community dynamics over the last 1200 years. We also used reconstructions of in-lake nutrients concentrations, air temperature fluctuations, and sedimentary organic matter dynamics to study impacts of past environmental and climatic changes on MOB community composition. DNA preservation in lake sediments is sufficient, and qPCR amplification was successfully applied to the analysis of MOB aDNA. Temporal changes in MOB community showed different patterns between lakes, and drivers of past MOB dynam…
Bioprospecting challenges in unusual environments
2017
Editorial: The microbiome as a source of new enterprises and job creation.
Constitutive activation of MexT by amino acid substitutions results in MexEF-OprN overproduction in clinical isolates of Pseudomonas aeruginosa
2018
ABSTRACT When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of Pseudomonas aeruginosa to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene mexT result in oligomerization of the LysR regulator MexT, constitutive upregulation of the efflux pump, and increased resistance in clinical isolates.
Phosphorylation of CENP-A on serine 7 does not control centromere function.
2019
CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viab…
TReND in Africa: Toward a Truly Global (Neuro)science Community
2020
TReND is a volunteer-scientist run charity dedicated to promoting research and education on the African continent. Focusing on neuroscience, we discuss approaches to address some of the factors that currently stifle Africa's scientific development and our experience in implementing them.
Role of AxyZ Transcriptional Regulator in Overproduction of AxyXY-OprZ Multidrug Efflux System in Achromobacter Species Mutants Selected by Tobramycin
2017
ABSTRACT AxyXY-OprZ is an RND-type efflux system that confers innate aminoglycoside resistance to Achromobacter spp. We investigated here a putative TetR family transcriptional regulator encoded by the axyZ gene located upstream of axyXY-oprZ . An in-frame axyZ gene deletion assay led to increased MICs of antibiotic substrates of the efflux system, including aminoglycosides, cefepime, fluoroquinolones, tetracyclines, and erythromycin, indicating that the product of axyZ negatively regulates expression of axyXY-oprZ . Moreover, we identified an amino acid substitution at position 29 of AxyZ (V29G) in a clinical Achromobacter strain that occurred during the course of chronic respiratory tract…
High-throughput sequencing for 1-methyladenosine (m1A) mapping in RNA
2016
Abstract Detection and mapping of modified nucleotides in RNAs is a difficult and laborious task. Several physico-chemical approaches based on differential properties of modified nucleotides can be used, however, most of these methods do not allow high-throughput analysis. Here we describe in details a method for mapping of rather common 1-methyladenosine (m1A) residues using high-throughput next generation sequencing (NGS). Since m1A residues block primer extension during reverse transcription (RT), the accumulation of abortive products as well as the nucleotide misincorporation can be detected in the sequencing data. The described library preparation protocol allows to capture both types …