Search results for "modified"
showing 10 items of 585 documents
A novel homologous model for noninvasive monitoring of endometriosis progression.
2017
To date, several groups have generated homologous models of endometriosis through the implantation of endometrial tissue fluorescently labeled by green fluorescent protein (GFP) or tissue from luciferase-expressing transgenic mice into recipient animals, enabling noninvasive monitoring of lesion signal. These models present an advantage over endpoint models, but some limitations persist; use of transgenic mice is laborious and expensive, and GFP presents poor tissue penetration due to the relatively short emission wavelength. For this reason, a homologous mouse model of endometriosis that allows in vivo monitoring of generated lesions over time and mimics human lesions in recipient mice wou…
Guidance on allergenicity assessment of genetically modified plants
2017
Abstract This document provides supplementary guidance on specific topics for the allergenicity risk assessment of genetically modified plants. In particular, it supplements general recommendations outlined in previous EFSA GMO Panel guidelines and Implementing Regulation (EU) No 503/2013. The topics addressed are non‐IgE‐mediated adverse immune reactions to foods, in vitro protein digestibility tests and endogenous allergenicity. New scientific and regulatory developments regarding these three topics are described in this document. Considerations on the practical implementation of those developments in the risk assessment of genetically modified plants are discussed and recommended, where …
Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells.
2005
Abstract PlOtp (Orthopedia), a homeodomain-containing transcription factor, has been recently characterized as a key regulator of the morphogenesis of the skeletal system in the embryo of the sea urchin Paracentrotus lividus . Otp acts as a positive regulator in a subset of oral ectodermal cells which transmit short-range signals to the underlying primary mesenchyme cells where skeletal synthesis is initiated. To shed some light on the molecular mechanisms involved in such a process, we begun a functional analysis of the cis -regulatory sequences of the Otp gene. Congruent with the spatial expression profile of the endogenous Otp gene, we found that while a DNA region from −494 to +358 is s…
Production of cecropin A in transgenic rice plants has an impact on host gene expression.
2008
Summary Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar ‘Senia’. Gene expression studies revealed that the expression of components of the protein secretory and vesicular transport machinery is co-ordinately activated at the pre-invasive stage of infection of rice by the blast fungus. Comparisons of gene expression between wild-type and cecropin A plants revealed the over-expression of genes involved in protection against oxidative stress in tran…
Surface Modified Arundo Donax Natural Fibers for Oil Spill Recovery
2021
The use of green materials for oil recovery applications is the goal to be achieved to reduce the environmental impact of these essential processes. In this context, Arundo Donax L. is a plant known for its wide uses whose absorbent properties have been previously investigated. In this paper, the influence of silane surface treatment on the absorbent behavior of natural fibers extracted from the culms of this eco-friendly and cost-effective material was assessed. A close correspondence has been identified between the physical characteristics of the investigated oil and the fiber size, by means of microstructural and morphological analysis. Excellent results have been achieved with an absorp…
A screening of five Bacillus thuringiensis Vip3A proteins for their activity against lepidopteran pests
2014
Five Bacillus thuringiensis Vip3A proteins (Vip3Aa, Vip3Ab, Vip3Ad, Vip3Ae and Vip3Af) and their corresponding trypsin-activated toxins were tested for their toxicity against eight lepidopteran pests: Agrotis ipsilon, Helicoverpa armigera, Mamestra brassicae, Spodoptera exigua, Spodoptera frugiperda, Spodoptera littoralis, Ostrinia nubilalis and Lobesia botrana. Toxicity was first tested at a high dose at 7 and 10. days. No major differences were found when comparing protoxins vs. trypsin-activated toxins. The proteins that were active against most of the insect species were Vip3Aa, Vip3Ae and Vip3Af, followed by Vip3Ab. Vip3Ad was non-toxic to any of the species tested. Considering the res…
Paratransgenic manipulation of tsetsemiR275alters the physiological homeostasis of the fly’s midgut environment
2021
AbstractTsetse flies are vectors of parasitic African trypanosomes (Trypanosomaspp.). Current disease control methods include fly-repelling pesticides, trapping flies, and chemotherapeutic treatment of infected people. Inhibiting tsetse’s ability to transmit trypanosomes by strengthening the fly’s natural barriers can serve as an alternative approach to reduce disease. The peritrophic matrix (PM) is a chitinous and proteinaceous barrier that lines tsetse’s midgut. It protects the epithelial cells from the gut lumen content such as food and invading trypanosomes, which have to overcome this physical barrier to establish an infection. Bloodstream form trypanosomes shed variant surface glycopr…
Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes
2011
Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 ± 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and str…
Effects of passive and active modified atmosphere packaging conditions on quality parameters of minimally processed table grapes during cold storage
2015
BACKGROUND: Table grape is a non-climacteric berry, sensitive to water loss and gray mold during postharvest storage. OBJECTIVE: To assess the effect of passive and active modified atmosphere packaging on quality parameters of minimally processed table grapes. METHODS: 'Red Globe' minimally processed table grapes were dipped in a sodium hypochlorite solution and irradiated with ultraviolet-C, before being packaged in a rigid polypropylene boxes, sealed with a micro perforated polypropylene film (PP) or a continuous polyethylene film, with: a) 5% O2 + 15% CO2 + 80% N (PET1); b) 20% CO2 + air (PET2). Samples were stored at 5°C for 21 days plus and additional 6-day period at 20°C to simulate s…
Effect of pressure on the electrochemical generation of hydrogen peroxide in undivided cells on carbon felt electrodes
2017
The electrochemical generation of H2O2can be performed in aqueous solutions by cathodic reduction at carbonaceous cathodes of oxygen coming from air. The performances of the process in terms of concentration of H2O2and current efficiency (CE) are limited by the low solubility of oxygen in water at atmospheric pressure. However, the solubility of oxygen can be increased upon enhancing the air pressure. The effect of pressure on the electrochemical generation of hydrogen peroxide was studied in undivided cells changing the pressure from 0 to 30 relative bar and the current density from 1 to 100Â mAÂ cmâ2. Simple carbon felt and compact graphite cathodes were first used. A dramatic effect of…