Search results for "mutants"

PPHD: une plateforme pour le phenotypage a haut debit

2012

International audience; The methodologies for genotyping and high throughput automated platforms for the production of type-omics (genomics, transcriptomics, protected omics ...) have greatly increased over the last few years and are now available to analyze the expression of genes of plants in several environmental conditions. However, a break of conceptual and technical services, non-destructive characterization and dynamic phenotypes, at the organ or plarite, has hundreds of related genotypes (Broadband) remains the limiting factor for the discovery of new characters or new varieties. This systematic exploration of diversity. Genetics within a species or between species requires platform…

Study of the adaptative response of N2 fixation to a root pruning in peas using environmental and genetic variations

2013

International audience; Pea is the first grain legume grown in Europe for its seeds rich in protein. As a legume, it has the ability to fix atmospheric N2 by the symbiosis with Rhyzobium bacteria. Still, N nutrition can be a limiting factor of pea yield, due to a high sensitivity of symbiotic N2 fixation to biotic stress like Aphanomyces that leads to root rots. The consequences of root damage on nodules and their N2 fixing activity and the possible adaptive response of plants still remain unknown. Root pruning could be an innovative experimental way to study these effects, resulting both in nodules and N2 deprivation. Still, researches described the adaptive responses of plants to a local …

Characterization of Pea (Pisum Sativum L.) genes implicated in arbuscular mycorrhiza formation and function

2010

The arbuscular mycorrhizal (AM) association results from a successful interaction between the genomes of the two symbiotic partners. In this context, the aim of my research was to better characterize the role of the late stage symbiosis-related pea genes PsSym36, PsSym33 and PsSym40 in the functional AM (i) by investigating the effect of mutations in the three genes on fungal and plant gene responses and (ii) by creating conditions for the localization of two of the genes, PsSym36 and PsSym40, on the pea genetic map for future map-based cloning. The expression of a subset of ten fungal and eight plant genes,previously reported to be activated during mycorrhiza development, was compared in G…

Contribution of proteomics to arbuscular mycorrhiza in Medicago truncatula

2007

International audience; Because proteins are key effectors of plant responses to environmental cues including recognition, signalling, transport and defence reactions, main interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. In our group, the setting up of high throughput proteomic techniques on the model species, Medicago truncatula, is providing step-by-step a large-scale analysis of AM symbiosis-related proteins. Depending on the symbiotic stage targeted and on the abundance of mycorrhizal material, different proteomic strategies that can be combined with other large-scale approaches (transcriptomic and meta…

Un facteur de transcription impliqué dans le développement de la graine chez Medicago truncatula

2013

Elpošanas ķēdes loma piruvātu producējošos Zymomonas mobilis celmos

2018

Lai novirzītu Zymomonas mobilis katabolismu no etanola producēšanas uz piruvāta producēšanu, jāatrod veids, kā oksidēt to NADH, kas parasti tiek izmantots etanola sintēzei, bet nav nepieciešams piruvāta producēšanai. Elpošana ir viens veidiem, kā oksidēt NADH. Lai gūtu priekšstatu par elpošanas ķēdes ietekmi uz piruvāta akumulēšanos Z. mobilis, tika salīdzināti vairāki elpošanas ķēdes mutantie celmi. Uz daļēji inaktivētas piruvātdekarboksilāzes (PDC) fona darbā izdevās iegūt celmu ar pārekspresētu membrānsaistīto D-laktātdehidrogenāzi (LDH), bet neizdevās iegūt celmu ar pārekspresētu NADH dehidrogenāzi. Novērojām, ka elpošanas ķēdes NADH dehidrogenāzes un LDH aktivitātes aerobi augošās Z.mo…

Blue-copper binding proteins of Medicago truncatula: Characterization of the expression of two genes related to the arbuscular mycorrhizal symbiosis

2009

International audience; Expression profiling of two paralogous arbuscular mycorrhizal (AM)-specific blue copper-binding gene (MtBcpla and MtBcp1b) isoforms was performed by real-time quantitative polymerase chain reaction in wild-type Medicago truncatula Jemalong 5 (J5) during the mycorrhizal development with Glomus intraradices for up to 7 weeks. Timecourse analysis in J5 showed that expression of both MtBcp1 genes increased continuously and correlated strongly with the colonization intensity and arbuscule content. MtPT4, selected as a reference gene of the functional plant-fungus association, showed a weaker correlation to mycorrhizal development. In a second experiment, a range of mycorr…

The effect of a temperature-sensitive prophage on the evolution of virulence in an opportunistic bacterial pathogen.

2022

https://doi.org/10.1111/mec.16638 Abstract Viruses are key actors of ecosystems and have major impacts on global biogeochemical cycles. Prophages deserve particular attention as they are ubiquitous in bacterial genomes and can enter a lytic cycle when triggered by environmental conditions. We explored how temperature affects the interactions between prophages and other biological levels by using an opportunistic pathogen, the bacterium Serratia marcescens, that harbours several prophages and that had undergone an evolution experiment under several temperature regimes. We found that the release of one of the prophages was temperature-sensitive and malleable to evolutionary changes. We furthe…

Homeostatic control of polyamine levels under long-term salt stress in Arabidopsis

2011

Salt stress has been frequently studied in its first osmotic phase. Very often, data regarding the second ionic phase is missing. It has also been suggested that Putrescine or/and Spermine could be responsible for salt resistance. In order to test this hypothesis under long-term salt stress, we obtained Arabidopsis thaliana transgenic plants harboring pRD29A::oatADC or pRD29A::GUS construction. Although Putrescine was the only polyamine significantly increased after salt acclimation in pRD29A::oatADC transgenic lines, this rendered in no advantage to this kind of stress. The higher Spermine levels found in WT and transgenic lines when compared to control conditions along with no increment o…