Search results for "nucleic acid"

showing 10 items of 810 documents

Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure

2002

A project group working under the European Confederation of Laboratory Medicine (ECLM) presents recommendations for standardized procedures for the Feulgen-Rossenbeck-Schiff and the periodic acid-Schiff (PAS) reactions on cytological and histological material. The advantages and disadvantages of such standardized procedures are presented here in a preamble. Both users and manufacturers are encouraged to give their opinions with a view to achieving consensus on these procedures and on how further work on these lines may proceed.

Pathologymedicine.medical_specialtyHistologyColoring agentsGeneral MedicinePeriodic acid–Schiff stainBiologyStainingMedical Laboratory TechnologymedicineMedical physicsFeulgen stainProject groupDesoxyribonucleic acidBiotechnic & Histochemistry
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HSV hepatitis in the mouse: A light and electron microscopic study with immunohistology and in situ hybridization

1988

In order to characterize better the morphology and immune response in acute necrotizing HSV infection, murine HSV hepatitis was examined. BALB/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (PFU) of HSV-1 (Lenette) and HSV-2 (D316). In both groups half the animals were pretreated with silica particles to block macrophage function. Up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron microscopy, immunohistology, in situ hybridization, combined immunohistology/in situ hybridization and titration of viral PFU. HSV-2 infected mice developed severe necrotizing hepatitis with persiste…

Pathologymedicine.medical_specialtyvirusesIn situ hybridizationBiologyVirus Replicationmedicine.disease_causeVirusLeukocyte CountMiceAlphaherpesvirinaemedicineAnimalsLymphocytesCytopathic effectHepatitisMice Inbred BALB CNucleic Acid HybridizationHerpes SimplexHistologyDNAGeneral Medicinemedicine.diseasebiology.organism_classificationImmunohistochemistryMicroscopy ElectronHerpes simplex virusLiverHepatitis Viral AnimalFemaleViral hepatitisVirchows Archiv B Cell Pathology Including Molecular Pathology
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Evaluation of a rapid antigen detection test (Panbio™ COVID‐19 Ag Rapid Test Device) as a point‐of‐care diagnostic tool for COVID‐19 in a pediatric e…

2021

Abstract We evaluated the Panbio™ COVID‐19 Ag Rapid Test Device as a point‐of‐care diagnostic tool for COVID‐19 in 357 patients at a pediatric emergency department. Thirty‐four patients tested positive by reverse transcription polymerase chain reaction, of which 24 were positive by the antigen assay. The sensitivity and specificity of the assay were 70.5% and 100%, respectively.

Pediatric emergencyMale2019-20 coronavirus outbreakmedicine.medical_specialtyCoronavirus disease 2019 (COVID-19)emergency departmentShort CommunicationShort CommunicationsSARS‐CoV‐2 RNA viral loadfield evaluationImmunologic TestsSensitivity and SpecificityCOVID-19 Serological TestingAntigenVirologyInternal medicineNasopharynxmedicineHumansProspective StudiesChildAntigens ViralPoint of carepediatric patientsbusiness.industrySARS-CoV-2clinical sensitivityCOVID-19InfantEmergency departmentVirologyTest (assessment)Reverse transcription polymerase chain reactionInfectious Diseasesrapid antigen assayPoint-of-Care TestingCOVID-19 Nucleic Acid TestingChild PreschoolFemalebusinessEmergency Service HospitalJournal of Medical Virology
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A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

2009

We describe a quantitative method for detecting RNA alternative splicing variants that combines in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes with confocal microscopy Förster resonance energy transfer (FRET). The use of PNA probes complementary to sequences flanking a given splice junction allows to specifically quantify, within the cell, the RNA isoform generating such splice junction by FRET measure. As a proof of concept we analyzed two alternative splicing events originating from lymphocyte antigen 6 (LY6) complex, locus G5B (LY6G5B) pre-mRNA. These are characterized by the removal of the first intron (Fully Spliced Isoform, FSI) or by retention of suc…

Peptide Nucleic AcidsGene isoformCytoplasmIn situ hybridizationBiologychemistry.chemical_compoundFluorescence Resonance Energy TransferGeneticsmedicineHumansProtein IsoformsspliceRNA MessengerIn Situ Hybridization FluorescenceMicroscopy ConfocalPeptide nucleic acidmedicine.diagnostic_testAlternative splicingIntronPepsin AAlternative SplicingNucleic Acid ProbesFörster resonance energy transferBiochemistrychemistryBiophysicsMethods OnlineCell NucleolusHeLa CellsFluorescence in situ hybridizationNucleic Acids Research
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Nanocarrier based on halloysite and fluorescent probe for intracellular delivery of peptide nucleic acids

2022

The development of systems able to deliver genetic material into a target site is a challenge for modern medicine. Single-stranded peptide nucleic acids have attracted attention as promising therapeutic molecules for diagnostic and gene therapy. However, their poor cell membrane permeability represents a drawback for biomedical applications. Halloysite nanotubes (HNTs) are emerging materials in drug delivery applications both for their ability to penetrate cell membranes and for enhancing the solubility of drugs in biological media. Herein, we report the first example of the use of a nanocarrier based on halloysite labelled with fluorescent switchable halochromic oxazine molecules, to deliv…

Peptide Nucleic AcidsNanotubesHalloysite nanotubesHalloysite nanotubes PNA Covalent modificationsSettore CHIM/06 - Chimica OrganicaHalochromic switchCovalent modificationsSettore CHIM/12 - Chimica Dell'Ambiente E Dei Beni CulturaliSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsBiomaterialsColloid and Surface ChemistryCell Line TumorCellular uptakeSettore BIO/14 - FarmacologiaClayPNAFluorescent Dyes
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The analysis of modified peroxisome proliferator responsive elements of the peroxisomal bifunctional enzyme in transfected HepG2 cells reveals two re…

1995

AbstractPeroxisome proliferators (PPs) are non-genotoxic carcinogens in rodents. They can induce the expression of numerous genes via the heterodimerization of two members of the steroid hormone receptor superfamily, called the peroxisome proliferator-activated receptor (PPAR) and the 9-cis retinoic acid receptor (RXR). Many of the PP responsive genes possess a peroxisome proliferator response element (PPRE) formed by two TGACCT-related motifs. The bifunctional enzyme (HD) PPRE contains 3 such motifs, creating DR1 and DR2 sequences. PPAR and RXR regulate transcription via the DR1 element while DR2 modulates the expression of the gene via auxiliary factors in HepG2 cells.

Peroxisome proliferator-activated receptor gammaReceptors Retinoic AcidSteroid hormone receptorMolecular Sequence DataResponse elementBiophysicsReceptors Cytoplasmic and NuclearPeroxisome proliferator-activated receptorchemical and pharmacologic phenomenaIn Vitro TechniquesRegulatory Sequences Nucleic AcidRetinoid X receptorBiologyPeroxisomal Bifunctional EnzymeTransfectionMicrobodiesBiochemistryGene Expression Regulation EnzymologicTranscriptional activationPeroxisomal Bifunctional EnzymeMultienzyme ComplexesStructural BiologyPeroxisome proliferator response element9-cis Retinoic acid receptor alphaTumor Cells CulturedGeneticsHumansRNA MessengerIsomerasesEnoyl-CoA HydrataseMolecular Biologychemistry.chemical_classificationBinding SitesBase Sequence3-Hydroxyacyl CoA DehydrogenasesPeroxisome proliferator-activated receptorCell BiologyDNA-Binding ProteinsRetinoic acid receptorRetinoid X ReceptorsLiverOligodeoxyribonucleotidesBiochemistrychemistryRat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenaseEnzyme InductionPeroxisome proliferator-activated receptor alphaTranscription FactorsFEBS Letters
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A new, easy and safe method for the purification of nucleic acids for reliable PCR

1994

PharmacologyCellular and Molecular NeuroscienceBiochemistryChemistryNucleic acid methodsNucleic acidMolecular MedicineCell BiologyMolecular BiologyExperientia
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Inulin Derivatives Obtained <i>Via</i> Enhanced Microwave Synthesis for Nucleic Acid Based Drug Delivery

2015

A new class of therapeutic agents with a high potential for the treatment of different socially relevant human diseases is represented by Nucleic Acid Based Drugs (NABD), including small interfering RNAs (siRNA), decoy oligodeoxynucleotides (decoy ODN) and antisense oligonucleotides (ASOs). Although NABD can be engineered to be specifically directed against virtually any target, their susceptibility to nuclease degradation and the difficulty of delivery into target tissues severely limit their use in clinical practice and require the development of an appropriate nanostructured delivery system. For delivery of NABD, Inulin (Inu), a natural, water soluble and biocompatible polysaccharide, wa…

PharmacologyNucleaseBiocompatibilitybiologyChemistryClinical BiochemistryCombinatorial chemistrychemistry.chemical_compoundBiochemistryDrug DiscoveryDrug deliveryNucleic acidbiology.proteinMolecular MedicineAgaroseAmine gas treatingLuciferaseCytotoxicityCurrent Drug Targets
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Transcriptional regulation and expression of CYP3A4 in hepatocytes.

2007

CYP3A4 is the most abundantly expressed drug-metabolizing P450 enzyme in human liver and contributes to the metabolism of a large number of drugs in use today. CYP3A4 is constitutively expressed in adult hepatocytes but it can also be transcriptionally induced by a variety of structurally diverse xenochemicals. CYP3A4 strongly contributes to the important variability in the therapeutic and toxic effects of drugs owing to the major role it plays in xenobiotic metabolism and the large intra- and inter-individual variability to which it is subjected. The functional examination of up to 13 kb of the CYP3A4 5'-flanking region has revealed that the regulation of this gene is a complex issue, with…

PharmacologyRegulation of gene expressionPregnane X receptorTranscription GeneticClinical BiochemistryDown-RegulationBiologyPharmacologyRegulatory Sequences Nucleic AcidGene Expression Regulation EnzymologicCell biologyDrug developmentNuclear receptorCytochrome P-450 Enzyme SystemLiverRegulatory sequenceTranscriptional regulationHepatocytesAnimalsCytochrome P-450 CYP3AHumansTranscription factorDrug metabolismCurrent drug metabolism
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Role of clays in protecting adsorbed DNA against X-ray radiation

2004

The X-ray emission of the young Sun was much harder and intense than today and might have played a significant role in the evolution of complex organics in protoplanetary environments. We investigate the effects of soft X-rays on tryptophan molecules in aqueous solutions at room temperature. As results of the irradiation experiments we detect several light species indicative of fragmentation, together with large molecular structures such as tryptophan dipeptide and tripeptide. Complexification is more evident in H2O solution than in D2O, probably due to isotopic effects. The abundances of peptides depend on the irradiation dose and decrease with increasing energy deposition. Radicals such a…

Physics and Astronomy (miscellaneous)X-rayX-ray irradiation of DNA clay minerals clay nucleic acid complexes surface mediated origin of genetic material origin of lifeRadiationPhotochemistrychemistry.chemical_compoundCrystallographyAdsorptionMontmorilloniteSettore FIS/05 - Astronomia E AstrofisicachemistrySpace and Planetary ScienceEarth and Planetary Sciences (miscellaneous)KaoliniteMoleculeClay mineralsEcology Evolution Behavior and SystematicsDNA
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