Search results for "oligo"

showing 10 items of 1298 documents

Identification of calcium sensing receptor (CaSR) mRNA-expressing cells in normal and injured rat brain

2009

Calcium sensing receptor (CaSR), isolated for the first time from bovine and human parathyroid, is a G-protein-coupled receptors that has been involved in diverse physiological functions. At present a complete in vivo work on the identification of CaSR mRNA-expressing cells in the adult brain lacks and this investigation was undertaken in order to acquire more information on cell type expressing CaSR mRNA in the rat brain and to analyse for the first time its expression in different experimental models of brain injury. The expression of CaSR mRNAs was found mainly in scattered cells throughout almost all the brain regions. A double labeling analysis showed a colocalization of CaSR mRNA expr…

Malemedicine.medical_specialtyTime FactorsCentral nervous systemHippocampusCell CountSettore BIO/11 - Biologia MolecolareBiologySettore BIO/09 - Fisiologiachemistry.chemical_compoundSeizuresInternal medicineSettore BIO/10 - BiochimicaCaSRmedicineAnimalsRNA MessengerRats WistarIbotenic AcidMolecular BiologyIn Situ HybridizationNeuronsKainic AcidGeneral NeuroscienceDentate gyrusBrainColocalizationImmunohistochemistryRatsOligodendrogliamedicine.anatomical_structureEndocrinologynervous systemchemistryBrain InjuriesNeurogliaNeurology (clinical)Pyramidal cellCaSR; BrainCalcium sensing receptor (CaSR) isolated for the first time from bovine and human parathyroid is a G-protein-coupled receptors that has been involved in diverse physiological functions. At present a complete in vivo work on the identification of CaSR mRNA-expressing cells in the adult brain lacks and this investigation was undertaken in order to acquire more information on cell type expressing CaSR mRNA in the rat brain and to analyse for the first time its expression in different experimental models of brain injury. The expression of CaSR mRNAs was found mainly in scattered cells throughout almost all the brain regions. A double labeling analysis showed a colocalization of CaSR mRNA expression in neurons and oligodendrocytes whereas it was not found expressed both in the microglia and in astrocytes. One week after kainate-induced seizure CaSR was found in the injured CA3 region of the hippocampus and very interestingly it was found up-regulated in the neurons of CA1-CA2 and dentate gyrus. Similarly 1 week following ibotenic acid injection in the hippocampus CaSR mRNA expression was increased in oligodendrocytes both in the lesioned area and in the contralateral CA1-CA3 pyramidal cell layers and dentate gyrus. One week after needle-induced mechanical lesion an increase of labeled cells expressing CaSR mRNA was observed along the needle track. In conclusion the present results contribute to extend available data on cell type-expressing CaSR in normal and injured brain and could spur to understand the role of CaSR in repairing processes of brain injury.Receptors Calcium-SensingIbotenic acidDevelopmental BiologyAstrocyte
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Targeting transcription factor Stat4 uncovers a role for interleukin-18 in the pathogenesis of severe lupus nephritis in mice

2011

Polymorphisms in the transcription factor Stat4 gene have been implicated as risk factors for systemic lupus erythematosus. Although some polymorphisms have a strong association with autoantibodies and nephritis, their impact on pathophysiology is still unknown. To explore this further we used signal transducers and activators of transcription 4 (Stat4) knockout MRL/MpJ-Fas(lpr)/Fas(lpr) (MRL-Fas(lpr)) mice and found that they did not differ in survival or renal function from Stat4-intact MRL-Fas(lpr) mice. Circulating interleukin (IL)-18 levels, however, were elevated in Stat4-deficient compared to Stat4-intact mice, suggesting that this interleukin might contribute to the progression of l…

Malemusculoskeletal diseasesMice Inbred MRL lprchronic inflammationLupus nephritisKidneyInterleukin-23ArticleProinflammatory cytokineOligodeoxyribonucleotides AntisenseGene Knockout TechniquesInterferon-gammaMiceimmune system diseasesmedicineAnimalsskin and connective tissue diseasesSTAT4DNA PrimersAutoimmune diseaseMice Knockoutlupus nephritisMice Inbred BALB CBase Sequencebusiness.industryGene Transfer TechniquesInterleukin-18InterleukinGlomerulonephritishemic and immune systemsSTAT4 Transcription Factormedicine.diseaseInterleukin-12chronic glomerulonephritisNephrologyImmunologyInterleukin 18FemalebusinessNephritisKidney International
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Expanded CTG repeats trigger miRNA alterations in Drosophila that are conserved in myotonic dystrophy type 1 patients

2013

Myotonic dystrophy type 1 (DM1) is caused by the expansion of CTG repeats in the 3' untranslated region of the DMPK gene. Several missplicing events and transcriptional alterations have been described in DM1 patients. A large number of these defects have been reproduced in animal models expressing CTG repeats alone. Recent studies have also reported miRNA dysregulation in DM1 patients. In this work, a Drosophila model was used to investigate miRNA transcriptome alterations in the muscle, specifically triggered by CTG expansions. Twenty miRNAs were differentially expressed in CTG-expressing flies. Of these, 19 were down-regulated, whereas 1 was up-regulated. This trend was confirmed for thos…

Malemusculoskeletal diseasescongenital hereditary and neonatal diseases and abnormalitiesDown-RegulationGene ExpressionBiologyMyotonic dystrophyLife ExpectancyGeneticsmedicineAnimalsDrosophila ProteinsHumansMyotonic DystrophyMuscle SkeletalMolecular BiologyCells CulturedGenetics (clinical)Oligonucleotide Array Sequence AnalysisGeneticsBase SequenceLife spanNuclear ProteinsGeneral Medicinemedicine.diseaseMicroRNAsDrosophila melanogasterGene Expression RegulationFemaleTranscriptomeTrinucleotide Repeat Expansion
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Involvement of plasmalogens in post-natal retinal vascular development

2014

Objective: Proper development of retinal blood vessels is essential to ensure sufficient oxygen and nutrient supplies to the retina. It was shown that polyunsaturated fatty acids (PUFAs) could modulate factors involved in tissue vascularization. A congenital deficiency in ether-phospholipids, also termed "plasmalogens'', was shown to lead to abnormal ocular vascularization. Because plasmalogens are considered to be reservoirs of PUFAs, we wished to improve our understanding of the mechanisms by which plasmalogens regulate retinal vascular development and whether the release of PUFAs by calcium-independent phospholipase A2 (iPLA2) could be involved. [br/] Methods and Results: By characterizi…

MaleretinaOrganes des sensAngiogenesis[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutritionlcsh:MedicineRetinal NeovascularizationBiochemistryImmunoenzyme TechniquesMicechemistry.chemical_compoundangiogenesisMedicine and Health Sciencesangiogenesis;astrocytes;capillaries;endothelial cells;gene expression;phospholipids;retina;retinal vesselscapillarieslcsh:ScienceCells CulturedOligonucleotide Array Sequence AnalysisMice KnockoutMultidisciplinarymedicine.diagnostic_testReverse Transcriptase Polymerase Chain ReactionLipidsendothelial cellsCell biologyEndothelial stem cellmedicine.anatomical_structureBiochemistry[ SDV.MHEP.OS ] Life Sciences [q-bio]/Human health and pathology/Sensory OrgansAlimentation et NutritionFatty Acids UnsaturatedRetinal DisordersFemaleResearch ArticleAstrocyteEndotheliumSensory OrgansPlasmalogensBiologyReal-Time Polymerase Chain ReactionGroup VI Phospholipases A2AngiopoietinElectroretinographymedicineFood and NutritionAnimalsRNA Messenger[SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory OrgansphospholipidsRetinaGene Expression Profilinglcsh:Rretinal vesselsastrocytesBiology and Life SciencesRetinalMice Inbred C57BLOphthalmologyAnimals Newbornchemistrygene expressionlcsh:QEndothelium Vascular[SDV.AEN]Life Sciences [q-bio]/Food and NutritionAcyltransferasesBiomarkersDevelopmental BiologyElectroretinography
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Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats

2020

Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice. Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, the effect of XOS on F. prausnitzii growth was assessed in vitro. Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group). XOS increased F. prausnitzii growth, having onl…

MalesuolistomikrobistoPROGRESSIONBIFIDOBACTERIASTEATOHEPATITISNon-alcoholic Fatty Liver DiseaseCecumDiet Fat-RestrictedaineenvaihduntaFatty Acidsrasvamaksafood and beveragesmitochondriaLiverprebioticBody CompositionBIOPSIESFemaleOxidation-Reductionlcsh:Nutrition. Foods and food supplymitokondriotGlucuronateslcsh:TX341-641Diet High-Fatdigestive systemArticleDYSBIOSISprebiootitINFLAMMATIONLIVER-DISEASEINTESTINAL MICROBIOTAoligosaccharidesoligosakkariditAnimalsRats WistarTriglyceridesfatty livergut microbiotaFaecalibacterium prausnitziinutritional and metabolic diseasesLipid MetabolismGastrointestinal MicrobiomeRatsFAECALIBACTERIUM-PRAUSNITZIIGlucosePrebiotics416 Food Scienceaineenvaihduntatuotteet3111 BiomedicineEnergy IntakeEnergy MetabolismmetabolismNutrients
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Defining the course of tumefactive multiple sclerosis: A large retrospective multicentre study

2021

Background and purpose: Tumefactive multiple sclerosis (TuMS) (i.e., MS onset presenting with tumefactive demyelinating lesions [TDLs]) is a diagnostic and therapeutic challenge. We performed a multicentre retrospective study to describe the clinical characteristics and the prognostic factors of TuMS. Methods: One hundred two TuMS patients were included in this retrospective study. Demographic, clinical, magnetic resonance imaging (MRI), laboratory data and treatment choices were collected. Results: TuMS was found to affect women more than men (female:male: 2.4), with a young adulthood onset (median age: 29.5 years, range: 11–68 years, interquartile range [IQR]: 38 years). At onset, 52% of …

Maletumefactive demyelinating lesions (TDLs)0302 clinical medicineRetrospective StudieInterquartile rangedifferential diagnosis030212 general & internal medicineProspective StudiesYoung adultProspective cohort studyChildtreatmentTumefactive multiple sclerosiTumefactive demyelinating lesionsDemyelinating DiseaseMiddle AgedMagnetic Resonance ImagingDifferential diagnosis Multiple sclerosis Tumefactive demyelinating lesions Tumefactive multiple sclerosisNeurologydifferential diagnosis; Multiple sclerosis; Tumefactive demyelinating lesions; Tumefactive multiple sclerosisFemaleHumanAdultmedicine.medical_specialtyMultiple SclerosisAdolescentdifferential diagnosiSettore MED/26Multiple sclerosis03 medical and health sciencesYoung AdultTumefactive multiple sclerosisOligoclonal BandInternal medicinemedicineHumansMultiple sclerosiTumefactive multiple sclerosisTumefactive multiple sclerosis (TuMS)AgedRetrospective StudiesTumefactive demyelinating lesionExpanded Disability Status Scalebusiness.industryOligoclonal BandsRetrospective cohort studyOdds ratiomedicine.diseaseConfidence intervalProspective Studiedifferential diagnosis; Multiple sclerosis; Tumefactive demyelinating lesions; Tumefactive multiple sclerosis; Adolescent; Adult; Aged; Child; Female; Humans; Magnetic Resonance Imaging; Male; Middle Aged; Oligoclonal Bands; Prospective Studies; Retrospective Studies; Young Adult; Demyelinating Diseases; Multiple SclerosisprognosisNeurology (clinical)business030217 neurology & neurosurgeryDemyelinating Diseases
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Exploring the Specificity of Rationally Designed Peptides Reconstituted from the Cell-Free Extract of Deinococcus radiodurans toward Mn(II) and Cu(II)

2020

A series of five rationally designed decapeptides [DEHGTAVMLK (DP1), THMVLAKGED (DP2), GTAVMLKDEH (Term-DEH), TMVLDEHAKG (Mid-DEH), and DEHGGGGDEH (Bis-DEH)] have been studied for their interactions with Cu(II) and Mn(II) ions. The peptides, constructed including the most prevalent amino acid content found in the cell-free extract of Deinococcus radiodurans (DR), play a fundamental role in the antioxidant mechanism related to its exceptional radioresistance. Mn(II) ions, in complex with these peptides, are found to be an essential ingredient for the DR protection kit. In this work, a detailed characterization of Cu(II) systems was included, because Cu(II)–peptide complexes have also shown r…

Manganesebiology010405 organic chemistryChemistryStereochemistryDeinococcus radioduransCell free010402 general chemistrybiology.organism_classification01 natural sciencesArticle0104 chemical sciencesInorganic ChemistryCoordination ComplexesDeinococcusPhysical and Theoretical ChemistryOligopeptidesCopperProtein BindingInorganic Chemistry
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Growth and sustainability of agricultural systems: the case of Sicilian wine-growing farms

2016

International audience; The Sicilian wine-growing sector is characterised by the presence on the one hand of many small enterprises that limit their activity to the first stage of the supply chain (field production) and on the other of few enterprises that adopt a strategy of total vertical integration, from the production to the sale of wine. The first group of enterprises operates in a competitive market and in many cases with marginal revenues that are lower than marginal costs, leading entrepreneurs to abandon the activity of grape production. The second group operates in an oligopolistic market and it is able to compete in an international market. Findings reveal that competitive advan…

Marginal costEconomics and EconometricscompetitiveneSupply chain0211 other engineering and technologies0507 social and economic geography02 engineering and technologyentrepreneurshipwine-growing farms.Competitive advantagelocal developmentOligopolyMarket economyAgrifood productSettore AGR/01 - Economia Ed Estimo RuraleEconomicsProduction (economics)Perfect competitionBusiness and International Managementeconomic sustainabilitySicilysupply chainSupply chain management[QFIN]Quantitative Finance [q-fin]05 social sciencesSMEsmall and medium enterprise021107 urban & regional planningCompetitor analysisrural territoryincome8. Economic growth050703 geography
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Les followers ont-ils vraiment de l'importance dans le modèle de Stackelberg?

2011

In this paper, we consider a T-stage linear model of Stackelberg oligopoly. First, we show geometrically and analytically that under the two conditions of linear market demand and identical constant marginal costs, the T-stage Stackelberg model reduces to a model where T oligopolies exploit residual demand sequentially. At any stage, leaders behave as if followers did not matter. Second, we study social welfare and convergence toward competitive equilibrium. Especially, we consider the velocity of convergence as the number of firms increases. The convergence is faster when reallocating firms from the most to the less populated cohort until equalizing the size of all cohorts.

Marginal costEconomics and Econometricsfollower's output indexíndice de producto del seguidorJEL: L - Industrial Organization/L.L1 - Market Structure Firm Strategy and Market Performance/L.L1.L13 - Oligopoly and Other Imperfect Marketsmodèle généralisé de Stackelberggeneralized Stackelberg competitioncompetencia de Stackelberg generalizadaCompetitive equilibriumrazón de descuento del markup del líderSupply and demandlcsh:Economic history and conditionsOligopolyjel:L20JEL : L - Industrial Organization/L.L1 - Market Structure Firm Strategy and Market Performance/L.L1.L13 - Oligopoly and Other Imperfect Marketseconomía linealgeneralized Stackelberg competition.Stackelberg competitionEconomicsLeader’s markup discount factor linear economy follower’s output discount factor myopic behavior[ SHS.ECO ] Humanities and Social Sciences/Economies and financesfacteurs d'escompte markupJEL : L - Industrial Organization/L.L2 - Firm Objectives Organization and Behavior/L.L2.L20 - General[SHS.ECO] Humanities and Social Sciences/Economics and FinanceHB71-74lcsh:HB71-74Economic history and conditionsLinear modellcsh:Economics as a scienceConvergence (economics)HC10-1085leader's markup discount ratio[SHS.ECO]Humanities and Social Sciences/Economics and FinanceGeneral Business Management and AccountingJEL: L - Industrial Organization/L.L2 - Firm Objectives Organization and Behavior/L.L2.L20 - GeneralEconomics as a sciencelinear economyjel:L13leader's markup discount ratio linear economy follower's output index generalized Stackelberg competitionlcsh:HC10-1085économie linéaireStatistics Probability and UncertaintyConstant (mathematics)Mathematical economicsFinanceSocial Sciences (miscellaneous)
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The beer market and advertising expenditure

2009

PurposeThe purpose of this paper is to examine the impacts of advertising expenditure on brands' market shares, utilizing a novel four‐week advertising‐sales data from the highly competitive oligopolistic Finnish beer market in which price competition among the homogeneous larger‐type beer brands is not allowed during the period of the study.Design/methodology/approachCompetition is modelled using the Lanchester model. The impacts of advertising on market shares are estimated using the impulse‐response functions from vector autoregression, and the full information maximum likelihood and advertising elasticities.FindingsSome new insights into beer market dynamics are obtained. First, the imp…

MarketingCompetition (economics)OligopolyHomogeneousValue (economics)EconomicsAdvertisingCompetitor analysisMarket dynamicsMarket shareVector autoregressionMarketing Intelligence & Planning
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