Search results for "ops"

showing 10 items of 5435 documents

Cancer-associated circulating large extracellular vesicles in cholangiocarcinoma and hepatocellular carcinoma.

2017

Background & Aims Large extracellular vesicles, specifically AnnexinV + EpCAM + CD147 + tumour-associated microparticles (taMPs), facilitate the detection of colorectal carcinoma (CRC), non-small cell lung carcinoma (NSCLC) as well as pancreas carcinoma (PaCa). Here we assess the diagnostic value of taMPs for detection and monitoring of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). Specifically, the aim of this study was to differentiate liver taMPs from other cancer taMPs, such as CRC and NSCLC. Methods Fluorescence-activated cell scanning (FACS) was applied to detect various taMP populations in patients' sera that were associated with the presence of a tumour (AnnexinV + Ep…

0301 basic medicineAdultMalePathologymedicine.medical_specialtyCirrhosisCarcinoma HepatocellularColorectal cancerAsialoglycoprotein ReceptorCholangiocarcinomaDiagnosis Differential03 medical and health scienceschemistry.chemical_compoundYoung Adult0302 clinical medicineCell-Derived MicroparticlesCell Line TumorCarcinomaBiomarkers TumorMedicineHumansLiquid biopsyAnnexin A5AgedHepatologybusiness.industryLiver NeoplasmsEpithelial cell adhesion moleculeHep G2 CellsMiddle Agedmedicine.diseaseEpithelial Cell Adhesion MoleculeTumor Burden030104 developmental biologychemistryBile Duct Neoplasms030220 oncology & carcinogenesisHepatocellular carcinomaCancer cellCancer researchBasiginFemalebusinessLiver cancerJournal of hepatology
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Endothelial cell damage is the central part of COVID-19 and a mouse model induced by injection of the S1 subunit of the spike protein☆

2021

Neurologic complications of symptomatic COVID-19 are common. Brain tissues from 13 autopsies of people who died of COVID-19 were examined. Cultured endothelial and neuronal cells were incubated with and wild type mice were injected IV with different spike subunits. In situ analyses were used to detect SARS-CoV-2 proteins and the host response. In 13/13 brains from fatal COVID-19, pseudovirions (spike, envelope, and membrane proteins without viral RNA) were present in the endothelia of microvessels ranging from 0 to 14 positive cells/200× field (mean 4.3). The pseudovirions strongly co-localized with caspase-3, ACE2, IL6, TNFα, and C5b-9. The surrounding neurons demonstrated increased NMDAR2…

0301 basic medicineAdultMalePathologymedicine.medical_specialtyProtein subunitH&E stainCaspase 3Spike proteinThirstPathology and Forensic Medicine03 medical and health sciencesMice0302 clinical medicineS1 subunitmedicineAnimalsHumansAgedAged 80 and overChemistrySARS-CoV-2COVID-19Endothelial CellsGeneral MedicineOriginal ContributionMiddle AgedMolecular biologyEndothelial stem cellDisease Models AnimalProtein Subunits030104 developmental biologyMembrane protein030220 oncology & carcinogenesisMicrovesselsSpike Glycoprotein CoronavirusImmunohistochemistryRNA ViralTumor necrosis factor alphaFemaleAutopsymedicine.symptomAnnals of Diagnostic Pathology
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The largest caucasian kindred with dentatorubral-pallidoluysian atrophy: A founder mutation in italy

2019

BACKGROUND: Dentatorubral-pallidoluysian atrophy is a hereditary neurodegenerative disease prevalently reported in Japan but rare in Caucasians. The objective of this study was to reconstruct the pedigree of Italian dentatorubral-pallidoluysian atrophy familial cases describing their clinical features. METHODS: We investigated 6 apparently unrelated dentatorubral-pallidoluysian atrophy families comprising a total of 51 affected individuals: 13 patients were clinically examined, and for 38 patients clinical data were collected from clinical sources. The dentatorubral-pallidoluysian atrophy diagnosis was genetically confirmed in 18 patients. Genealogical data from historical archives were ana…

0301 basic medicineAdultMalePediatricsmedicine.medical_specialtyAdolescentDiseaseNeuropsychological TestsWhite People03 medical and health sciencesEpilepsyYoung Adult0302 clinical medicineAtrophyTrinucleotide Repeatsdentatorubral-pallidoluysian atrophymedicineHumansFamilyATN1 geneChildFounder mutationAgedDentatorubral-pallidoluysian atrophyEpilepsybusiness.industrygenealogical methodMiddle Agedmedicine.diseaseMyoclonic Epilepsies ProgressivePedigree030104 developmental biologyfounder effectNeurologyCerebellar cognitive affective syndromeItalycerebellar cognitive-affective syndromeMutationFemaleNeurology (clinical)business030217 neurology & neurosurgeryFounder effect
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Clinical course of sly syndrome (mucopolysaccharidosis type VII).

2016

WOS: 000377110800007

0301 basic medicineAdultMalePediatricsmedicine.medical_specialtyAdolescentMucopolysaccharidosisSly syndromeHepatosplenomegalyMetabolic disordersMucopolysaccharidosis VIIMedical and Health Sciences03 medical and health sciencesYoung Adult0302 clinical medicineHydrops fetalisSurveys and QuestionnairesmedicineGeneticsHumansMedical history1506Clinical geneticsFamily historyPreschoolChildGenetics (clinical)GlucuronidaseGenetics & Hereditybusiness.industryGenotype-Phenotype CorrelationsMucopolysaccharidosis VIIInfantEnzyme replacement therapyBiological Sciencesmedicine.diseaseLysosomal Storage Diseases030104 developmental biologyPhenotypeClinical genetics Genetics Metabolic disordersChild PreschoolFemalemedicine.symptombusiness030217 neurology & neurosurgeryMPS ; lysosomal storage disease ; β-glucuronidase
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Long-term physical activity modulates brain processing of somatosensory stimuli: Evidence from young male twins.

2016

Leisure-time physical activity is a key contributor to physical and mental health. Yet the role of physical activity in modulating cortical function is poorly known. We investigated whether precognitive sensory brain functions are associated with the level of physical activity. Physical activity history (3-yr-LTMET), physiological measures and somatosensory mismatch response (sMMR) in EEG were recorded in 32 young healthy twins. In all participants, 3-yr-LTMET correlated negatively with body fat%, r = −0.77 and positively with VO2max, r = 0.82. The fat% and VO2max differed between 15 physically active and 17 inactive participants. Trend toward larger sMMR was seen in inactive compared to ac…

0301 basic medicineAdultMalehuman electrophysiologyFITNESSMISMATCH NEGATIVITY MMN515 PsychologyPhysical activityMonozygotic twinprecognitive brain functionPhysical exerciseSensory systemCHILDRENGatingElectroencephalographyMotor ActivitySomatosensory system03 medical and health sciences0302 clinical medicinephysical exercisemedicineEVOKED-POTENTIALSHumansHealthy Lifestyleta315Evoked PotentialsExerciseYoung malemedicine.diagnostic_testLATENCYGeneral NeuroscienceMEMORYta3141ADULTSSomatosensory Cortex16. Peace & justice3142 Public health care science environmental and occupational healthta3124030104 developmental biologyNeuropsychology and Physiological PsychologyDISCRIMINATIONFemalePsychologyNeurosciencesomatosensory mismatch response030217 neurology & neurosurgeryRESPONSESBiological psychology
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The Gut Metagenome Changes in Parallel to Waist Circumference, Brain Iron Deposition, and Cognitive Function.

2017

Context Microbiota perturbations seem to exert modulatory effects on emotional behavior, stress-, and pain-modulation systems in adult animals; however, limited information is available in humans. Objective To study potential relationships among the gut metagenome, brain microstructure, and cognitive performance in middle-aged, apparently healthy, obese and nonobese subjects after weight changes. Design This is a longitudinal study over a 2-year period. Setting A tertiary public hospital. Patients or other participants Thirty-five (18 obese) apparently healthy subjects. Intervention(s) Diet counseling was provided to all subjects. Obese subjects were followed every 6 months. Main outcome me…

0301 basic medicineAdultMalemedicine.medical_specialtyWaistEndocrinology Diabetes and MetabolismIronClinical BiochemistryHippocampusFirmicutesContext (language use)BiologyNeuropsychological TestsBiochemistryAmygdala03 medical and health sciences0302 clinical medicineEndocrinologyCognitionInternal medicinemedicineImage Processing Computer-AssistedHumansEffects of sleep deprivation on cognitive performanceLongitudinal StudiesObesityBacteroidetesBiochemistry (medical)Case-control studyBrainCognitionMiddle AgedMagnetic Resonance ImagingGastrointestinal Microbiome030104 developmental biologyEndocrinologymedicine.anatomical_structureCross-Sectional StudiesCase-Control StudiesMetagenomeFemaleWaist CircumferenceBody mass index030217 neurology & neurosurgeryTenericutesThe Journal of clinical endocrinology and metabolism
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Alpha-defensins (α-Defs) in Crohn's disease: decrease of ileal α-Def 5 via permanent methylation and increase in plasma α-Def 1-3 concentrations offe…

2018

Summary An impaired expression of α-defensins (α-Defs) in the ileal mucosa and, conversely, increased levels in plasma, have been reported in Crohn's disease (CD). However, the specificity and correlation of these findings with the degree of inflammation are unclear. We aimed to characterize the concentration and utility of ileal and plasma α-Defs in CD and to analyse a potential epigenetic mechanism of α-Def expression. Peripheral blood samples and ileal biopsies were obtained from patients at disease onset (aCD), from those who achieved remission (iCD) and from two control groups (healthy controls and non-CD-aetiology ileitis patients). Plasma α-Defs 1–3 and 4 were detected by enzyme-link…

0301 basic medicineAdultMalemedicine.medical_specialtyalpha-DefensinsAdolescentBiopsyImmunologyAlpha (ethology)InflammationGastroenterologyMethylationEpigenesis Genetic03 medical and health sciencesYoung Adult0302 clinical medicineIntestinal mucosaCrohn DiseaseIleumInternal medicineBiopsyImmunology and AllergyMedicineHumansIleitisRNA MessengerIntestinal MucosaAgedInflammationCrohn's diseasemedicine.diagnostic_testbusiness.industryMethylationOriginal ArticlesMiddle Agedmedicine.disease030104 developmental biologyEndocrinologyCase-Control StudiesBiomarker (medicine)030211 gastroenterology & hepatologyFemalemedicine.symptombusinessBiomarkersClinical and experimental immunology
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Associations between neuropsychological performance and appetite-regulating hormones in anorexia nervosa and healthy controls: Ghrelin's putative rol…

2019

Anorexia nervosa (AN) is a severe eating disorder accompanied by alterations in endocrinological circuits and deficits in neuropsychological performance. In this study, a series of appetite-regulating hormones (ghrelin, leptin, cholecystokinin, PYY, adiponectin, and visfatin) were measured under fasting conditions in female patients with AN and female healthy controls. All of the participants also underwent a battery of neuropsychological assessment [namely the Iowa Gambling Task (IGT), the Wisconsin Card Sorting Test (WCST), and the Stroop Color and Word Test (SCWT)]. As the main finding, we found that higher ghrelin levels predict better performance in the IGT. Ghrelin may be a putative m…

0301 basic medicineAdultmedicine.medical_specialtyAnorexia Nervosamedia_common.quotation_subjectDecision MakingAppetite030209 endocrinology & metabolismNeuropsychological TestsBiochemistryModels BiologicalCohort Studies03 medical and health sciencesYoung Adult0302 clinical medicineEndocrinologyWisconsin Card Sorting TestInternal medicineAppetite regulationmedicineHumansNeuropsychological assessmentMolecular Biologymedia_commonmedicine.diagnostic_testbusiness.industryLeptindigestive oral and skin physiologyAppetiteAnorexia nervosaIowa gambling taskNeuropsychological performanceGhrelin030104 developmental biologyEndocrinologyAnorexia nervosa (differential diagnoses)Case-Control StudiesGhrelinbusinesshormones hormone substitutes and hormone antagonistsStroop effectDecision-making
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The CD68+/H-ferritin+ cells colonize the lymph nodes of the patients with adult onset Still's disease and are associated with increased extracellular…

2015

Summary In this work, we aimed to evaluate the levels of ferritin enriched in H subunits (H-ferritin) and ferritin enriched in L subunits (L-ferritin) and the cells expressing these two molecules in the lymph node (LN) biopsies obtained from adult-onset Still's disease (AOSD) patients, and the possible correlation among these data and the severity of the disease. Ten patients with AOSD underwent LN biopsy. All the samples were stained by immunofluorescence. A statistical analysis was performed to estimate the possible correlation among both H-ferritin and L-ferritin tissue expression and the clinical picture of the disease. Furthermore, the same analysis was performed to evaluate the possib…

0301 basic medicineAdult-OnsetMalePathologyMacrophageApoferritinAdult-onset Still's disease; H-ferritin; Hyperferritinaemic syndrome; Macrophage; Adult; Aged; Antigens CD; Antigens Differentiation Myelomonocytic; Apoferritins; Biopsy; Female; Ferritins; Fluorescent Antibody Technique; Humans; Lymph Nodes; Macrophages; Male; Middle Aged; Still's Disease Adult-Onset; Immunology; Immunology and AllergyH-ferritinBiopsyFluorescent Antibody TechniquePathogenesis0302 clinical medicineMacrophageImmunology and AllergyLymph nodemedicine.diagnostic_testCD68Lymph NodeMiddle AgedCDmedicine.anatomical_structureAntigenDifferentiationFemaleLymphHyperferritinaemic syndromeStill's Disease Adult-OnsetHumanAdultmedicine.medical_specialtyImmunologyAntigens Differentiation MyelomonocyticBiologyImmunofluorescenceAdult-onset Still's disease03 medical and health sciencesAntigens CDBiopsymedicineHumansAntigensAged030203 arthritis & rheumatologyFerritinMacrophagesOriginal ArticlesMyelomonocyticStill's DiseaseFerritinSettore MED/16 - Reumatologia030104 developmental biologyImmunologyApoferritinsFerritinsbiology.proteinLymph Nodes
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Peripheral Maintenance of the Axis SIRT1-SIRT3 at Youth Level May Contribute to Brain Resilience in Middle-Aged Amateur Rugby Players

2019

Physical exercise performed regularly is known to improve health and to reduce the risk of age-related diseases. Furthermore, there is some evidence of cognitive improvement in physically active middle-aged and older adults. We hypothesized that long-term physically active middle-aged men may have developed brain resilience that can be detected with the analysis of peripheral blood markers. We aimed to analyze the activation of pathways potentially modulated by physical activity in a cohort of healthy amateur rugby players (n = 24) and control subjects with low physical activity (n = 25) aged 45¿65 years. We had previously reported neuropsychological improvement in immediate memory response…

0301 basic medicineAgingBrain resilienceMiddle-aged and young menCognitive Neurosciencemedia_common.quotation_subjectPhysiologyPhysical exerciseExerciciSanglcsh:RC321-571SIRT303 medical and health scienceswhole-blood gene expression0302 clinical medicineSIRT1Whole-blood gene expressionphysical exerciseMedicineCervellmiddle-aged and young menExerciselcsh:Neurosciences. Biological psychiatry. Neuropsychiatrymedia_commonWhole bloodOriginal Researchbusiness.industrybrain resilienceLongevityNeuropsychologyBrainPhysical exerciseCognitionExecutive functionsEsportistes d'elit030104 developmental biologyBloodAthletesCohortPsychological resiliencebusiness030217 neurology & neurosurgeryNeuroscienceFrontiers in Aging Neuroscience
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