Search results for "pOR"

Nuclear localization but not PML protein is required for incorporation of the papillomavirus minor capsid protein L2 into virus-like particles.

2004

ABSTRACT Recent reports suggest that nuclear domain(s) 10 (ND10) is the site of papillomavirus morphogenesis. The viral genome replicates in or close to ND10. In addition, the minor capsid protein, L2, accumulates in these subnuclear structures and recruits the major capsid protein, L1. We have now used cell lines deficient for promyelocytic leukemia (PML) protein, the main structural component of ND10, to study the role of this nuclear protein for L2 incorporation into virus-like particles (VLPs). L2 expressed in PML protein knockout (PML −/− ) cells accumulated in nuclear dots, which resemble L2 aggregates forming at ND10 in PML protein-containing cells. These L2 assemblies also attracted…

Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations.

2001

ABSTRACT Studies of the Hepatitis C virus (HCV) replication cycle have been made possible with the development of subgenomic selectable RNAs that replicate autonomously in cultured cells. In these replicons the region encoding the HCV structural proteins was replaced by the neomycin phosphotransferase gene, allowing the selection of transfected cells that support high-level replication of these RNAs. Subsequent analyses revealed that, within selected cells, HCV RNAs had acquired adaptive mutations that increased the efficiency of colony formation by an unknown mechanism. Using a panel of replicons that differed in their degrees of cell culture adaptation, in this study we show that adaptive…

Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.

1992

Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.

Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry

2012

ABSTRACT Adeno-associated virus type 2 (AAV2) has gained much interest as a gene delivery vector. A hallmark of AAV2-mediated gene transfer is an intracellular conformational change of the virus capsid, leading to the exposure of infection-relevant protein domains. These protein domains, which are located on the N-terminal portion of the structural proteins VP1 and VP2, include a catalytic phospholipase A 2 domain and three clusters of basic amino acids. We have identified additional protein sequence motifs located on the VP1/2 N terminus that also proved to be obligatory for virus infectivity. These motifs include signals that are known to be involved in protein interaction, endosomal sort…

Single amino acid substitutions in the glycoprotein B carboxy terminus influence the fusion from without property of herpes simplex virus type 1.

1995

Syncytial mutations of herpes simplex virus type 1 (HSV-1) strains ANG, ANG path, HFEM, tsB5 and HSZP cause extensive cell fusion and were mapped to the cytoplasmic domain of glycoprotein B (gB), within the syn 3 locus. These strains are so far the only ones which show the phenotype ‘fusion from without’ (FFWO): 60 min after infection with high m.o.i., cells in a tissue culture are fused without transcription and translation of the viral genome. In this report we detected, using the recombinants 27/III and K-7, that an amino acid exchange from Ala to Val at aa position 854 of gB is the main determinant for FFWO activity of strains ANG, ANG path and recombinant K-7. The transfer of this muta…

PTC124-mediated translational readthrough of a nonsense mutation causing Usher syndrome type 1C.

2011

We investigated the therapeutic potential of the premature termination codon (PTC) readthrough-inducing drug PTC124 in treating the retinal phenotype of Usher syndrome, caused by a nonsense mutation in the USH1C gene. Applications in cell culture, organotypic retina cultures, and mice in vivo revealed significant readthrough and the recovery of protein function. In comparison with other readthrough drugs, namely the clinically approved readthrough-inducing aminoglycoside gentamicin, PTC124 exhibits significant better retinal biocompatibility. Its high readthrough efficiency in combination with excellent biocompatibility makes PTC124 a promising therapeutic agent for PTCs in USH1C, as well a…

Ultrasound-guided viscosupplementation in glenohumeral microinstability in sports athletes

2014

Glie et neurones : vers un partenariat actif influençant le comportement sensoriel chez la drosophile

2014

Fenêtres sur couples : trois nouvelles de Sean O'Faolain

2003

In several short stories, Sean O'Faolain ponders over the complexity of love relationships and tries to unveil some of the unconscious motivations underlying them. This essay focuses on three of these short stories, namely "There's a Birdie in the Cage", "Discord" and "An Inside Outside Complex", and shows how, in each of them, the fissures or cracks in love relationships are figuratively represented by an isotopy of windows, the various qualities of which are artfully exploited by O'Faolain (the window as partition between contiguous spaces, as transparent filter, as surface or frame). The position of characters with regard to windows (their framing) and their relations to windows (how the…

Izturības īpašību attīstīšana futbolā pusaudžiem

2022

Futbolistu pusaudžu treniņu procesam un metodiskajiem paņēmieniem ir jābūt tik kvalitatīviem, lai tie sekmētu un attīstītu fizisko īpašību izturību. Ir daudzas metodes kā var trenēt izturības veidus. Kvalifikācijas darba tēma ir “Izturības īpašību attīstīšana futbolā pusaudžiem”. Kvalifikācijas darba mērķis ir- noskaidrot Madonas BJSS MT-6 futbola pusaudžu treniņgrupas izturības trenētību. Izstrādājot kvalifikācijas darbu, tika izmantotas sekojošas pētīšanas metodes- sporta treniņu teorijas, metodikas, sporta fizioloģijas, biomehānikas literatūras avotu pētīšana un analīze; aptaujas metode- intervija; pedagoģiskais novērojums; kvalifikācijas darba autors tika veicis kontrolvingrinājumus izt…