Search results for "palm"
showing 10 items of 338 documents
Analisi molecolare per lo studio della variabilità genetica nel Punteruolo rosso
2009
Al fine di caratterizzare e confrontare le popolazioni di Rhynchophorus ferrugineus presenti in Sicilia, con quelle presenti negli areali autoctoni e di recente introduzione, sono stati messi a punto dei protocolli per l’analisi di specifiche sequenze del DNA genomico di questo fitofago. Sono stati disegnati i primer da utilizzare per l’amplificazione della regione ITS del punteruolo rosso. In particolare, per l’amplificazione della porzione ITS1 un primer forward è posizionato nella porzione terminale del gene 18S (P1) e l’altro nella porzione iniziale del gene 5,8S (P2), con opposta direzione di sintesi.Per l’amplificazione dell’ITS2, invece, i primer forward e reverse sono posizionati, r…
Bacillus thuringiensis treatment modulate the HSP70 expression in larva and adult brain of Rhynchophorus ferrugineus
2013
To study the pathogen-host relationship, we used the model of the entomopathogenic bacterium Bacillus thuringiensis (Bt) and Rhynchophorus ferrugineus, a quarantine pest that attacks palm trees. In particular, we focused on the Bt stress-induced infections. We studied the effect of Bt on larval and adult growth, and on the expression of the heat shock proteins (HSPs), rapidly synthesized in the cell after exposure to stress including pathogens. Bt has negative effects on larval and adult growth, on total hemocytes counts and on the hemocyte type. HSP70 was evaluated in the supernatant of the brain lysate obtained from larvae and adults fed with sublethal doses of Bt. HSP70 expression was mo…
Association of entomopathogenic fungi with exotic red palm weevil in treated and untreated Phoenix canariensis
2011
Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) is introduced pests of established Phoenix canariensis in Sicily (Italy). A three-year field survey of R. ferrugineus generated data on species of fungal associated with larval, pupae, and adult R. ferrugineus recovered from several cadavers. Moreover specimens were collected from infested Phoenix canariensis in scattered locales in Sicily to determine also infection rates with entomopathogenic fungi over 3 years. Collection was done after overwintering period, during summer and at the beginning of autumn in palm, untreated and treated with chemical pesticides. As can be expected the highest infestation was recorded after overw…
Potential effects of Bacillus thuringiensis against adults and older larvae of Rhynchophorus ferrugineus.
2009
Liposomes as nonspecific nanocarriers for 5-Fluorouracil in the presence of cyclodextrins
2021
Abstract 5-Fluorouracil (5-FU) is one of anticancer drugs with broad activity. Due to its severe side effects, recent studies concentrate on new ways of directed 5-FU delivery and its release in ill tissue. One of selective carriers could be cyclodextrins and liposomes. The combination of novel methods, leading to formation of inclusion complexes (IC) between host molecule of β-cyclodextrin (β-CD) or 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and 5-FU guest and its subsequent encapsulation in dipalmitoylphosphatidylcholine (DPPC) liposomes is studied experimentally in the present work. Several methods are applied to proof the encapsulation of the analysed drug and its release over time at 37 …
Mboat7 down-regulation by hyper-insulinemia induces fat accumulation in hepatocytes.
2020
Background: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved in phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between hepatic Mboat7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2 hepatocytes. Findings: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammatio…
NATURAL OCCURRENCE AND CHARACTERISTICS OF ENTOMOPATHOGENIC FUNGI ISOLATED FROM RED PALM WEEVIL IN SICILY (ITALY).
2008
Introduction: Originally from tropical Asia, the red palm weevil (Rhynchophorus ferrugineus (Olivier)) is the most dangerous and deadly pest of date, coconut, oil, sago and other palms. It has spread to Africa and Europe, reaching the Mediterranean in the 1980s, and was first recorded in Sicily (Italy) in 2005. Due to its recent introduction and the difficulty of its control, to known the natural pathogens of RPW in the introduced areas is very important. Methods: Pupae from infested palms Phoenix canariensis Hort. were collected. The entomopathogenic fungi were isolated and growth. Because of it is possible to growth only the 1% of the known fungi, the identification of entomopathogens is …
Preliminary studies on red palm weevil as potential risk for palm trees in mediterranean basin
2012
The impacts of insect invasive species on human health and on agricutlture have attracted worldwide attention. However, researchers and policymakers rarely directly address the connection between invasive species and loss in natual and cultural heritages. Various attempts have been made to address the plants that are affected by invasive species but the links between these and loss in naturla biodiversity and heritage in botanical and historical gardens are largely lacking in the literature. We report a study on the Red Palm Weevil (RPW), Rhynchophorous ferrugineus (Oliv.) (Fig. 1) (Coleoptera, Curculionidae) which is the most important pest of the palm date trees in many countries. RPW is …
INDAGINI SULLA RISPOSTA IMMUNITARIA DEL PUNTERUOLO ROSSO NEI CONFRONTI DEI NEMATODI ENTOMOPAGENI
2009
Il Punteruolo rosso delle palme (Rhynchophorus ferrugineus Olivier, 1790. Coleoptera Curculionidae), originario dell’Asia meridionale si è velocemente diff uso nell’areale mediterraneo attaccando diversi generi di palme e creando seri problemi al settore ornamentale e vivaistico, oltre che alle aree verdi delle città. Il controllo di questo fi tofago si è rivelato problematico e di diffi cile realizzazione a causa delle sue proprietà invasive. Allo stato attuale non sono stati individuati patogeni effi caci nella lotta biologica, anche se i nematodi entomopatogeni (EPN) sono stati impiegati con discreto successo in Spagna. Generalmente gli EPN sono impiegati contro gli stadi larvali ma poic…
Antimicrobial activity of the red palm weevil Rhynchophorus ferrugineus
2011
The red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera Dryophthoridae), is an important pest of palms. Knowl- edge of both its natural enemies and its defensive mechanisms against predators and microorganisms is important to develop methods for an integrated pest control. Antimicrobial activity of the cuticular surface of adults and larvae, as well as of eggs, of this invasive species was investigated. This activity was tested against the Gram-positive bacteria Bacillus subtilis (Ehrenberg) Cohn and Bacillus thuringiensis Berliner, the Gram-negative bacterium Escherichia coli Escherich, and the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium aniso…