Search results for "pcr"
showing 10 items of 438 documents
Origin of neuronal-like receptors in Metazoa: cloning of a metabotropic glutamate/GABA-like receptor from the marine sponge Geodia cydonium.
1999
To date, no conclusive evidence has been presented for the existence of neuronal-like elements in Porifera (sponges). In the present study, isolated cells from the marine sponge Geodia cydonium are shown to react to the excitatory amino acid glutamate with an increase in the concentration of intracellular calcium [Ca2+]i. This effect can also be observed when the compounds L-quisqualic acid (L-QA) or L-(+)-2-amino-4-phosphonobutyric acid (L-AP-4) are used. The effect of L-QA and L-AP-4, both agonists for metabotropic glutamate receptors (mGluRs), can be abolished by the antagonist of group I mGluRs, (RS)-alpha-methyl-4-carboxyphenylglycine. These data suggest that sponge cells contain an mG…
Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro
2021
IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-t…
On Extremal Cases of the Hopcroft's Algorithm
2010
In this paper we consider the problem of minimization of deterministic finite automata (DFA) with reference to Hopcroft’s algorithm. Hopcroft’s algorithm has several degrees of freedom, so there can exist different executions that can lead to different sequences of refinements of the set of the states up to the final partition. We find an infinite family of binary automata for which such a process is unique, whatever strategy is chosen. Some recent papers (cf. Berstel and Carton (2004) [3], Castiglione et al. (2008) [6] and Berstel et al. (2009) [1]) have been devoted to find families of automata for which Hopcroft’s algorithm has its worst execution time. They are unary automata associated…
Comparative analysis of Hsp10 and Hsp90 expression in healthy mucosa and adenocarcinoma of the large bowel.
2014
Heat shock proteins (Hsps) assist other proteins in their folding and drive the degradation of defective proteins. During evolution, these proteins have also acquired other roles. Hsp10 is involved in immunomodulation and tumor progression. Hsp90 stabilizes a range of "client" proteins involved in cell signaling. The present study evaluated the expression levels of Hsp10 and Hsp90 in normal mucosa and adenocarcinoma samples of human large bowel.Samples of normal mucosa and adenocarcinoma were collected and Reverse transcriptase-polymerase chain reaction RT-PCR, western blotting (WB) analyses, as well as immunohistochemistry were performed to evaluate the expression levels of Hsp10 and Hsp90…
Green sulphur bacteria as a component of the photosynthetic plankton community in small dimictic humic lakes with an anoxic hypolimnion
2013
High bacteriochlorophyll (BChl) concentrations in the anoxic water layers of some humic lakes have indicated that green sulphur bacteria (GSB) may be ecologically significant. The abundance and spatial distribution of GSB were therefore addressed in 13 small humic lakes using fragment analysis and sequencing of PCR-amplified 16S rRNA genes. GSB were detected from lakes where the photosynthetically active radiation was at least 1.1 µE m-2 s-1 at the oxic-anoxic boundary layer. In these lakes, 13 to 42% of the 16S rRNA gene sequences of the anoxic water column were assigned to GSB. The spatial distribution of GSB was tightly correlated with the spectrophotometrically measured BChl concentrati…
Isolation and characterisation of Nocardioides sp. SP12, an atrazine-degrading bacterial strain possessing the gene trzN from bulk- and maize rhizosp…
2003
International audience; We report the characterisation of Nocardioides sp. SP12, an atrazine-degrading bacteria isolated from atrazine-treated bulk- and maize rhizosphere soil. Based on 16S rDNA alignment, strain SP12 showed close phylogenic relationships with Nocardioides sp. C157 and Nocardioides simplex. Internal transcribed spacer (ITS) sequences of strain SP12 were longer than those of other Nocardioides sp. and present Ala- and Ile-tRNA unlike Actinomycetales. Nocardioides sp. SP12 presents a novel atrazine catabolic pathway combining trzN with atzB and atzC. Atrazine biodegradation ends in a metabolite that co-eluted in HPLC with cyanuric acid. This metabolite shows an absorption spe…
Profile of P-glycoprotein distribution in the rat and its possible influence on the salbutamol intestinal absorption process.
2004
8 pages, 2 figures, 2 tables.--PMID: 15124220 [PubMed]
Discrimination of non-infectious SARS-CoV-2 particles from fomites by viability RT-qPCR.
2021
The ongoing coronavirus 2019 (COVID-19) pandemic constitutes a concerning global threat to public health and economy. In the midst of this pandemic scenario, the role of environment-to-human COVID-19 spread is still a matter of debate because mixed results have been reported concerning SARS-CoV-2 stability on high-touch surfaces in real-life scenarios. Up to now, no alternative and accessible procedures for cell culture have been applied to evaluate SARS-CoV-2 infectivity on fomites. Several strategies based on viral capsid integrity have latterly been developed using viability markers to selectively remove false-positive qPCR signals resulting from free nucleic acids and damaged viruses. T…
New CpCrCl2(PR3) complexes: physical properties and reduction chemistry
1998
Abstract Compounds CpCrCl2L ( L = PMe 2 Ph , 1 , PMePh 2 , 2 ; PPh 3 , 3 ) have been prepared. Their magnetic moment is in accord with the presence of three unpaired electron and a variable temperature 1H NMR investigation of 1 shows Curie-Weiss behavior. The 1H NMR, EPR and UV-visible spectra are in agreement with those of other previously reported compounds of the same family. An electrochemical investigation shows the accessibility of an irreversible reduction process. A parallel sodium reduction investigation of complexes CpCrCl2L (L = PMe3 and η1-dppe) suggests tjat the reduction process is followed by immediate chloride loss, and then by a ligand redistribution process to afford chrom…
Plasma cell-free DNA in diagnostic KRAS mutation testing
2015
Epidermaalisen kasvutekijän reseptorin (EGFR) monoklonaalisia vasta-aineita (mAb) käytetään apuna metastaattisen kolorektaalisyövän (mCRC) hoidossa. Aktivoivat mutaatiot KRAS- ja NRAS-onkogeeneissä tekevät syöpäsolut vastustuskykyisiksi anti-EGFR-vasta-aineille, ja tästä syystä tuumorit tulee genotyypittää ennen hoidon aloittamista. Kudosbiopsian ottaminen on kuitenkin invasiivinen toimenpide, eikä näyte välttämättä edusta kattavasti syövän kaikkien solupopulaatioiden geneettisiä muutoksia. Verenkierron solunulkoisen DNA:n (cfDNA) on havaittu sisältävän maligneista soluista lähtöisin olevia onkogeenisiä mutaatioita, ja se voi olla hyvä vaihtoehtoinen tuumoriperäisen DNA:n lähde. Kehitimme j…