Search results for "pcr"

Bioaugmentācijas izmantošana farmācijas notekūdeņu attīrīšanas efektivitātes uzlabošanai

2015

Šajā darbā tika pētīta no farmācijas attīrīšanas iekārtām izdalītu, dabisku mikroorganismu spēja degradēt specifiskus farmakoloģijas savienojumus tīrkultūru veidā, kā arī bioaugmentācijas procesā simulētos reālos vide apstākļos. Izmantotās metodes ļauj salīdzinoši novērtēt mikroorganismu koncentrāciju un dinamiku vidē. Tika parādīts, ka mikroorganismu aktivitāte testa sistēmas apstākļos ir būtiski atšķirīga no in vitro. Konstatēts, ka bioaugmentācijas celmiem ir augsta izskalošanas pakāpe un ir nepieciešams veikt papildus pasākumus, lai to samazinātu. Ir grūti paredzēt pievienoto mikroorganismu efektu uz eksistējošo mikrofloru. Sēnei Gibberella indica ir augsts potenciāls tikt izmantotai fa…

Associazione di più fattori di rischio e d’elevati livelli di fibrinogeno e PCR: ruolo predittivo di complicanze vascolari in una popolazione di sogg…

2003

RELAZIONE FRA I LIVELLI EMATICI DI PCR, FIBRINOGENO C E MMP-9 E DIFFERENTI PATTERN CLINICI DELL'ARITMIA

2010

Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain

2013

Teschovirus and Sapelovirus are two genera of the Picornaviridae family, comprising highly variable and heterogeneous enteric viruses, commonly found in faecal samples from domestic pigs. Although both of them are also known to infect wild boar, studies on their presence in these wild suids are scarce. The present study aimed at determining the presence of porcine teschovirus (PTV) and sapelovirus (PSV) in free-living wild boar populations, as well as to study their relationships with similar viruses present in pigs. Fresh faecal samples (n = 63) from wild boar were collected in Doñana Biological Reserve (SW Spain) during 2007 and 2011, and analysed using multiplex RT-PCR for the simultaneo…

Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR

2011

Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen (R) method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affec…

HCV vīrusa genomu CORE/ARFP reģiona sekvenču analīze

2015

C hepatīta vīruss (HCV) – pieder flavivīrusu dzimtai. HCV ir apvalku saturošs vīruss. HCV var izraisīt tikai cilvēka un augstāko primātu slimības. C hepatīta vīrusa (HCV) ierosināta infekcija ir pasaulē izplatīts iemesls hroniskām aknu slimībām. Pret HCV vēl nav izstrādāta vakcīna. HCV ir pirmais vīruss, kas ir atklāts ar molekulārās klonēšanas palīdzību, bez tiešas bioloģisko vai biofizikālo metožu palīdzības. Galvenā HCV vīrusa īpatnība ir izteikta ģenētiskā mainība. ARFP (alternatīvās nolasīšanas rāmja proteīns) jeb F (nobīdes), jeb core +1 proteīns (17 kDa) ir radies ribosomu -2/+1 nobīdes rezultātā N-gala core -kodēšanas reģionā HCV poliproteīnā. Joprojām nav zināma ARFP bioloģiskā fun…

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

2022

Background: the most widely distributed and virulent Grapevine leafroll-associated viruses (GLRaV) that affect grapevine are GLRaV-1 and GLRaV-3, transmitted semi-persistently by different mealybugs and soft scales, mainly causing downward rolling of the leaf margins and interveinal reddening. Methods: the main objectives of this study were to investigate the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in 617 samples from 11 autochthonous Sicilian grapevine cultivars, ascertaining their presence and spread. The detection was implemented by serological and molecular analyses and subsequently phylogenetic analyses on selected Sicilian isolates were conducted. Results: in …

Evolutionary Analysis of Grapevine Virus A: Insights into the Dispersion in Sicily (Italy)

2022

Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by the scale insect Neopulvinaria innumerabilis. In this work, we studied the genetic structure and molecular variability of GVA, ascertaining its presence and spread in different commercial vineyards of four Sicilian provinces (Italy). In total, 11 autochthonous grapevine cultivars in 20 commercial Sicilian vineyards were investigated, for a total of 617 grapevine samples. Preliminary screening by serological (DAS-ELISA) analysis for GV…

easyPAC: A Tool for Fast Prediction, Testing and Reference Mapping of Degenerate PCR Primers from Alignments or Consensus Sequences

2012

Video abstract A video abstract by the authors of this paper is available. video-abstract8870.mov

Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts

2021

Abstract Interactions among symbiotic organisms and their hosts are major drivers of ecological and evolutionary processes. Monitoring the infection patterns among natural populations and identifying factors affecting these interactions are critical for understanding symbiont–host relationships. However, many of these interactions remain understudied since the knowledge about the symbiont species is lacking, which hinders the development of appropriate tools. In this study, we developed a digital droplet PCR (ddPCR) assay based on apicomplexan COX1 gene to detect an undescribed agamococcidian symbiont. We show that the method gives precise and reproducible results and enables detecting cryp…