Search results for "pero"
showing 10 items of 3365 documents
Improvement in Nuclear Entry and Transgene Expression of Baculoviruses by Disintegration of Microtubules in Human Hepatocytes
2005
ABSTRACT Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), a potent virus for mammalian cell gene delivery, possesses an ability to transduce mammalian cells without viral replication. We examined the role of the cellular cytoskeleton in the cytoplasmic trafficking of viral particles toward the nucleus in human hepatic cells. Microscopic studies showed that capsids were found in the nucleus after either viral inoculation or cytoplasmic microinjection of nucleocapsids. The presence of microtubule (MT) depolymerizing agents caused the amount of nuclear capsids to increase. Overexpression of p50/dynamitin, an inhibitor of dynein-dependent endocytic trafficking from peripheral e…
Incorporation and metabolism of trans 20∶5 in endothelial cells. Effect on prostacyclin synthesis
2000
To study the ability of long-chain trans fatty acids (FA) to be incorporated and metabolized into endothelial cells, bovine aortic endothelial cells were incubated with medium enriched eicosapentaenoic acid (EPA) bound to albumin (M2) or one of its geometrical isomers: 20:5 5c,8c,11t,14c,17c (M3), 20:5 5c,8c,11c,14c,17t (M4), or 20:5 5c,8c,11t,14c,17t (M5). After 48 h of incubation, supernatant and cells were harvested and their lipids were analyzed, including prostacyclin synthesis. EPA and 22:5n-3 of endothelial cells incubated with M2 were, respectively, three and two times higher than in control cells (incubated in M1, without any fatty acid added), whereas 22:6n-3 increased only in the…
High levels of the molecular chaperone Mdg1/ERdj4 reflect the activation state of endothelial cells
2003
Mdg1/ERdj4, a mammalian chaperone that belongs to the HSP40 protein family, has been reported to be located in the endoplasmic reticulum (ER), is induced by ER stress, and protects ER stressed cells from apoptosis. Here we show that under normal physiological conditions, Mdg1/ERdj4 is expressed at various levels in the vasculature due to different activation states of the endothelium. To elucidate the stimuli that induce ER stress and thus upregulate Mdg1/ERdj4, we investigated the effect of several endothelium specific stressors on its expression. Mdg1/ERdj4 mRNA is induced by activated macrophages, by nitric oxide (NO) and heat shock, and during terminal cell differentiation, whereas shea…
Reconstruction of the Rib Vaults of the Hypostyle Hall in the Maniace Castle of Siracusa After the 1693 Earthquake
2016
ABSTRACTBuilt in the first half of the 13th century on behalf of the Emperor Frederick II, the Maniace Castle of Siracusa focuses its enigma in the hypostyle hall (which was composed of 25 quadripartite rib vaults) and the design criteria adopted for its implementation. Recent research and a careful rereading of the documentary “restoration project” after the earthquake of 1693 have offered new tools to understand the construction techniques and building procedures for the entire organism. It has been possible to demonstrate that the different material (white limestone and volcanic stone) used for the vaults is, in fact, a product of reconstruction. The constructive node, which historiograp…
Comparative genomic and phylogenetic analyses of gammaproteobacterial glg genes traced the origin of the Escherichia coli glycogen glgBXCAP operon to…
2015
© 2015 Almagro et al. Production of branched α-glucan, glycogen-like polymers is widely spread in the Bacteria domain. The glycogen pathway of synthesis and degradation has been fairly well characterized in the model enterobacterial species Escherichia coli (order Enterobacteriales, class Gammaproteobacteria), in which the cognate genes (branching enzyme glgB, debranching enzyme glgX, ADP-glucose pyrophosphorylase glgC, glycogen synthase glgA, and glycogen phosphorylase glgP) are clustered in a glgBXCAP operon arrangement. However, the evolutionary origin of this particular arrangement and of its constituent genes is unknown. Here, by using 265 complete gammaproteobacterial genomes we have …
Evaluation of the effects of titanium dioxide and aluminum oxide nanoparticles through tarsal contact exposure in the model insect Oncopeltus fasciat…
2019
Abstract Despite the increasing presence of metal nanoparticles in the biosphere as a consequence of their widespread use, knowledge about the impact of these nanoparticles on fauna, ecosystems and human health is far from completion. This is especially true for terrestrial invertebrates. Insects are environmentally exposed to nanoparticles by several ways, the ectopic contact being one of the most probable. The model insect Oncopeltus fasciatus, has been used in the present work for testing toxicity of nanoparticles present in a surface. Adverse effects of TiO2 nanoparticles and Al2O3 in nanoparticulated or bulk form on mortality, reproductive and embryonic developmental parameters have be…
Time-dependent effects of polystyrene nanoparticles in brine shrimp Artemia franciscana at physiological, biochemical and molecular levels
2019
Micro- (<5 mm) and nanoplastics (<1 μm) are emerging threats for marine ecosystems worldwide. Brine shrimp Artemia is recognized as a suitable model among planktonic species for studying the impact of polystyrene nanoparticles (PS NPs) through short and long-term bioassays. Our study aims to evaluate the time-dependent effects of cationic amino-modified PS-NH (50 nm) in A. franciscana after short- (48 h) and long-term exposure (14 days). For this purpose, nauplii were exposed to a concentration range of PS-NH (0.1, 1, 3 and 10 μg/mL) in natural sea water (NSW), and physiological, biochemical and molecular responses were investigated. Short-term exposure to PS-NH caused a decrease in nauplii…
Comparison of organic peracids in wastewater treatment: Disinfection, oxidation and corrosion.
2015
The use of organic peracids in wastewater treatment is attracting increasing interest. The common beneficial features of peracids are effective anti-microbial properties, lack of harmful disinfection by-products and high oxidation power. In this study performic (PFA), peracetic (PAA) and perpropionic acids (PPA) were synthesized and compared in laboratory batch experiments for the inactivation of Escherichia coli and enterococci in tertiary wastewater, oxidation of bisphenol-A and for corrosive properties. Disinfection tests revealed PFA to be a more potent disinfectant than PAA or PPA. 1.5 mg L(-1) dose and 2 min of contact time already resulted in 3.0 log E. coli and 1.2 log enterococci r…
Oxidation of diclofenac in the presence of iron(II) octacarboxyphthalocyanine
2021
Abstract This paper presents the results of the research on the influence of catalytic activity of iron(II) octacarboxyphthalocyanines (FePcOC) on the transformation of diclofenac (DCF) which is the most popular anti-inflammatory analgesic. Diclofenac poses a serious threat to the natural environment. The paper demonstrates that diclofenac, in the presence a monomeric form of iron octacarboxyphthalocyanine and hydroxyl radicals (HO•) (from H2O2), undergoes a transformation into diclofenac-2,5-iminoquinone (DCF-2,5-IQ), causing distinct changes in the UV–Vis absorption spectrum. In the presence of iron octacarboxyphthalocyanine and H2O2, the previously colourless diclofenac solution becomes …
The use of anthracene as a model compound in a comparative study of hydrous pyrolysis methods for industrial waste remediation
2011
Author's version of an article published in Chemosphere, 84 (4), 403-408. Also available from the publisher: http://dx.doi.org/10.1016/j.chemosphere.2011.03.061 Polycyclic aromatic hydrocarbons are very stable compounds and tend to bioaccumulate in the environment due to their high degree of conjugation and aromaticity. Hydrous pyrolysis is explored as a technique for the treatment of industrial water containing PAH, using anthracene as a model compound. The reactivity of anthracene under a range of temperatures and durations are studied in this paper. Aliquots of 1.0-10.0mg of anthracene in a range of 1.0-5.0 mL of H(2)O are subjected to hydrous pyrolysis under varied conditions of tempera…