Search results for "pero"
showing 10 items of 3365 documents
Functional and conformational properties of the exclusive C-domain from the Arabidopsis copper chaperone (CCH)
2001
The Arabidopsis thaliana copper chaperone (CCH) is a small copper binding protein involved in copper trafficking. When compared to homologues from other eukaryotes, CCH has two different domains; the conserved N-domain and the plant-exclusive C-domain, a C-terminal extension with an unusual amino-acid composition. In order to characterize this extra C-domain, the CCH protein, the N-domain and the C-domain were all expressed separately in heterologous systems. While the N-domain retained the copper chaperone and antioxidant properties described for the yeast Atx1 and human HAH1 counterparts, the C-domain displayed particular structural properties that would be necessary to optimize copper ho…
Chromogranin A in the Mammalian Merkel Cell: Cellular and Subcellular Distribution
1989
Chromogranin-A (CGA), which accounts for more than half the soluble matrix protein in secretory granules of various neuroendocrine cells, has a wide spectrum of potential biological roles and is considered an important marker of the diffuse neuroendocrine system (DNES). Light and electron microscopic immunohistochemistry of mammalian skin revealed that Merkel cells are exclusively CGA-immunoreactive (ir) and that the immunoreaction is localized in the secretory granules. This finding supports the classification of the Merkel cell as a member of the DNES. The CGA immunoreactivity was restricted to Merkel cells of pigs and humans. In human embryonic skin, CGA was expressed in Merkel cells as …
Role of Antioxidants in Cooled Liquid Storage of Mammal Spermatozoa
2021
Cooled preservation of semen is usually associated with artificial insemination and genetic improvement programs in livestock species. Several studies have reported an increase in reactive oxidative species and a decrease in antioxidant substances and sperm quality parameters during long-term semen storage at refrigerated temperatures. The supplementation of antioxidants in extenders before refrigeration could reduce this detrimental effect. Various antioxidants have been tested, both enzymatic, such as superoxide dismutase and catalase, and non-enzymatic, such as reduced glutathione, vitamins E and C and melatonin. However, the problem of oxidative stress in semen storage has not been full…
2004
Background Arabidopsis thaliana copper metallochaperone CCH is a functional homologue of yeast antioxidant ATX1, involved in cytosolic copper transport. In higher plants, CCH has to be transported to specialised cells through plasmodesmata, being the only metallochaperone reported to date that leaves the cell where it is synthesised. CCH has two different domains, the N-terminal domain conserved among other copper-metallochaperones and a C-terminal domain absent in all the identified non-plant metallochaperones. The aim of the present study was the biochemical and biophysical characterisation of the C-terminal domain of the copper metallochaperone CCH.
Daidzein has neuroprotective effects through ligand-binding-independent PPARγ activation.
2011
Phytoestrogens are a group of plant-derived compounds that include mainly isoflavones like daidzein. Phytoestrogens prevent neuronal damage and improve outcome in experimental stroke; however, the mechanisms of this neuroprotective action have not been fully elucidated. In this context, it has been postulated that phytoestrogens might activate the peroxisome proliferator-activated receptor-γ (PPARγ), which exerts neuroprotective effects in several settings. The aim of this study was to determine whether the phytoestrogen daidzein elicits beneficial actions in neuronal cells by mechanisms involving activation of PPARγ. Our results show that daidzein (0.05-5 μM) decreases cell death induced b…
A New Mutation in the Promoter Region of the PAX8 Gene Causes True Congenital Hypothyroidism with Thyroid Hypoplasia in a Girl with Down's Syndrome
2014
Thyroid dysfunction is common in newborn infants with Down's syndrome (DS), but defects causing classic thyroid dysgenesis (TD) with permanent congenital hypothyroidism (CH) have not been described.We studied a girl with DS and CH who had a mutation in the promoter sequence of the PAX8 gene.A female infant was found to have trisomy 21 and CH, with a venous thyrotropin (TSH) of150 mU/L and a free thyroxine (fT4) of 15.1 pmol/L (day 12). Thyroid peroxidase antibodies and thyroglobulin antibodies were elevated. Scintigraphy showed normal uptake, but ultrasound identified a small gland with heterogenous echotexture and cystic changes. Sequence analysis of the PAX8 gene revealed a new heterozygo…
In vitro and in vivo detection of end-products of MPO-driven oxidative and nitrosative stress in human endocardial endothelial cells from patients af…
2008
In vivo evaluation of the effects of 10% carbamide peroxide and 3.5% hydrogen peroxide on the enamel surface
2007
Objectives: Bleaching of vital teeth performed at home by the patient under the dentist’s supervision, using low-concentration peroxides and custom-fitted trays specifically designed for this purpose, is one of several options for this type of dental treatment, whether alone or in combination with another in-office bleaching technique. The objective of this study is to analyse the effect on the enamel surface of two bleaching products recommended for this technique. Materials & methods: Two bleaching products were used: VivaStyle (Vivadent), a 10% carbamide peroxide, and FKD (Kin), a 3.5% hydrogen peroxide. They were applied in trays to the anterior teeth of 20 patients (10 in each group). …
Estudio de los valores de referencia para los parámetros de estrés oxidativo: malondialdehído y glutatión, medidos por cromatografía líquida de alta …
2015
La investigación en estrés oxidativo ha experimentado una expansión masiva en los últimos años. Multitud de datos indican que la producción de especies reactivas en los sistemas biológicos regulan numerosos procesos fisiológicos. La producción descontrolada de estas especies está implicada en el inicio y propagación de diversas patologías. Una aproximación común para la determinación del estrés oxidativo en los sistemas biológicos consiste en la medida del aumento o el descenso de moléculas redox-sensibles que responden a este tipo de estrés. Los biomarcadores de estrés oxidativo usualmente se clasifican en 4 categorías. La primera engloba la detección de oxidantes. Desafortunadamente, la m…