Search results for "pero"
showing 10 items of 3365 documents
Effects of tributyltin(IV) chloride on the gametes and fertilization ofAscidia malaca (Ascidiacea: Tunicata)
2003
Ascidia malaca gametes before fertilization incubated in 10-5 or 10-7 M solutions of tributyltin(IV) chloride, TBTCl, for 3 h appear highly damaged under transmission electron microscopy observation. Also, the fertilization process is affected by the compound: the damaged spermatozoa are present in the vitelline coat and the egg does not cleave. An increase of microbodies, structurally similar to peroxisomes, have been detected in the egg peripheral cytoplasm, probably in relation to their role in alleviating damage to some cellular components. The results have shown that the reproduction of ascidians under unfavourable environmental conditions is prevented. Copyright © 2003 John Wiley &…
Haemoglobin in ascitic fluid increases lipid peroxidation in acute pancreatitis
2012
Causative role of oxidative stress in a Drosophila model of Friedreich ataxia
2006
Friedreich ataxia (FA), the most common form of hereditary ataxia, is caused by a deficit in the mitochondrial protein frataxin. While several hypotheses have been suggested, frataxin function is not well understood. Oxidative stress has been suggested to play a role in the pathophysiology of FA, but this view has been recently questioned, and its link to frataxin is unclear. Here, we report the use of RNA interference (RNAi) to suppress the Drosophila frataxin gene (fh) expression. This model system parallels the situation in FA patients, namely a moderate systemic reduction of frataxin levels compatible with normal embryonic development. Under these conditions, fh-RNAi flies showed a shor…
Deferiprone and idebenone rescue frataxin depletion phenotypes in a Drosophila model of Friedreich's ataxia
2013
Friedreich's ataxia (FRDA), the most common inherited ataxia, is a neurodegenerative disease caused by a reduction in the levels of the mitochondrial protein frataxin, the function of which remains a controversial matter. Several therapeutic approaches are being developed to increase frataxin expression and reduce the intramitochondrial iron aggregates and oxidative damage found in this disease. In this study, we tested separately the response of a Drosophila RNAi model of FRDA ( Llorens et al., 2007) to treatment with the iron chelator deferiprone (DFP) and the antioxidant idebenone (IDE), which are both in clinical trials. The FRDA flies have a shortened life span and impaired motor coord…
Senataxin defective in ataxia oculomotor apraxia type 2 is involved in the defence against oxidative DNA damage
2007
Adefective response to DNA damage is observed in several human autosomal recessive ataxias with oculomotor apraxia, including ataxia-telangiectasia. We report that senataxin, defective in ataxia oculomotor apraxia (AOA) type 2, is a nuclear protein involved in the DNA damage response. AOA2 cells are sensitive to H2O2, camptothecin, and mitomycin C, but not to ionizing radiation, and sensitivity was rescued with full-length SETX cDNA. AOA2 cells exhibited constitutive oxidative DNA damage and enhanced chromosomal instability in response to H2O2. Rejoining of H2O2-induced DNA double-strand breaks (DSBs) was significantly reduced in AOA2 cells compared to controls, and there was no evidence fo…
Diurnal variability, photochemical production and loss processes of hydrogen peroxide in the boundary layer over Europe
2019
Hydrogen peroxide (H2O2) plays a significant role in the oxidizing capacity of the atmosphere. It is an efficient oxidant in the liquid phase and serves as a temporary reservoir for the hydroxyl radical (OH), the most important oxidizing agent in the gas phase. Due to its high solubility, removal of H2O2 due to wet and dry deposition is efficient, being a sink of HOx (OH+HO2) radicals. In the continental boundary layer, the H2O2 budget is controlled by photochemistry, transport and deposition processes. Here we use in situ observations of H2O2 and account for chemical source and removal mechanisms to study the interplay between these processes. The data were obtained during five ground-base…
Die autoxydation von linolsäuremethylesterhydroperoxyd. Über die autoxydation ungesättigter verbindungen. XIII. Mitteilung
1961
Linolsauremethylesterhydroperoxyd wurde mit Hilfe der O'KEEFFE-Verteilung aus autoxydiertem Linolsauremethylester isoliert. Die Autoxydation dieses Hydroperoxydes verlauft rascher als die des Linolsaureesters. Die Reaktion verlaft aber nicht autokatalytisch und ist nach Aufnahme von 1 Mol Sauerstoff pro Mol Hydroperoxyd praktisch beendet; die Hydroperoxydgruppen bleiben dabei zum grosten Teil erhalten, die konjugierten Doppelbindungen verschwinden. Die Aktivierungsenergie dieser Reaktion ist um 3 kcal/Mol kleiner als die der Autoxydation des Linolsauremethylesters. Es wird ein Mechanismus vorgeschlagen, bei dem das Linolsauremethylesterhydroperoxyd hauptsachlich in biomolekularer Startreakt…
The role of the co-chaperone BAG3 in selective macroautophagy: implications for aging and disease
2012
Maintenance of protein homeostasis, correct protein folding, refolding and clearance is of central importance for the function and survival of every cell. Here, the degradation of proteins is of particular importance, especially during aging and certain degenerative disorders when the protein load is increased. During cellular aging as well as under acute stress, there is a reciprocal change in expression of two members of the BAG (Bcl-2-associated athanogene) family, BAG1 and BAG3. While BAG1 serves an important function during the degradation of ubiquitinated proteins via the proteasome, BAG3 is the mediator of a novel macroautophagy pathway. This BAG3-mediated macroautophagy is based on …
Involvement of PAR-4 in Cannabinoid-Dependent Sensitization of Osteosarcoma Cells to TRAIL-Induced Apoptosis
2014
The synthetic cannabinoid WIN 55,212-2 is a potent cannabinoid receptor agonist with anticancer potential. Experiments were performed to determine the effects of WIN on proliferation, cell cycle distribution, and programmed cell death in human osteosarcoma MG63 and Saos-2 cells. Results show that WIN induced G2/M cell cycle arrest, which was associated with the induction of the main markers of ER stress (GRP78, CHOP and TRB3). In treated cells we also observed the conversion of the cytosolic form of the autophagosome marker LC3-I into LC3-II (the lipidated form located on the autophagosome membrane) and the enhanced incorporation of monodansylcadaverine and acridine orange, two markers of t…
Abitare sociale e governance territoriale: quali strumenti per la rigenerazione urbana?
2013
La questione abitativa oggi ha assunto un ruolo di notevole importanza nei processi decisionali di trasformazione urbana; nonostante la drammaticità del tema, rimane un compito molto arduo individuare una coerenza strutturale in merito alla storia delle politiche di edilizia residenziale pubblica. L’auto-recupero, l’auto-costruzione, l’alloggio condiviso sono alcune delle forme più interessanti fra le politiche e le strategie dell’alloggio sociale, poiché rappresentano delle risposte specifiche e concrete al bisogno di abitazione a buon mercato e costituiscono un valido strumento nei processi di governance territoriale.