Search results for "phoretic"

showing 10 items of 59 documents

Beta-MSH inhibits brain inflammation via MC(3)/(4) receptors and impaired NF-kappaB signaling.

2005

The anti-inflammatory effects of melanocortin peptides have been demonstrated in different inflammation models. This is the first report describing the molecular mechanisms for the beta-MSH-induced suppression of bacterial lipopolisaccharide (LPS)-caused brain inflammation. We found that beta-MSH suppresses LPS-induced nuclear translocation of the transcription factor NF-kappaB, and inhibits the expression of inducible nitric oxide synthase, and the following nitric oxide overproduction in the brain, in vivo. Moreover, administering the preferentially MC(4) receptor selective antagonist HS014 blocked completely these effects, suggesting a tentative MC(4) receptor mediated mechanism of actio…

LipopolysaccharidesMalemedicine.medical_specialtyImmunologyNitric Oxide Synthase Type IIInflammationElectrophoretic Mobility Shift AssayNitric OxidePeptides CyclicNitric oxidechemistry.chemical_compoundMiceInternal medicinebeta-MSHmedicineImmunology and AllergyAnimalsDrug InteractionsReceptorBrain ChemistryMice Inbred ICRbiologyDose-Response Relationship DrugImmunochemistryElectron Spin Resonance SpectroscopyNF-kappa BNF-κBHormonesCell biologyNitric oxide synthaseDisease Models AnimalEndocrinologyNeurologyMechanism of actionchemistrybiology.proteinEncephalitisReceptor Melanocortin Type 4Neurology (clinical)medicine.symptomMelanocortinSignal transductionhormones hormone substitutes and hormone antagonistsReceptor Melanocortin Type 3Signal TransductionJournal of neuroimmunology
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Acute exercise activates nuclear factor (NF)-kappaB signaling pathway in rat skeletal muscle.

2004

Two studies were performed to investigate the effects of an acute bout of physical exercise on the nuclear protein kappaB (NF-kappaB) signaling pathway in rat skeletal muscle. In Study 1, a group of rats (n=6) was run on the treadmill at 25 m/min, 5% grade, for 1 h or until exhaustion (Ex), and compared with a second group (n=6) injected with two doses of pyrrolidine dithiocarbamate (PDTC, 100 mg/kg, i.p.) 24 and 1 h prior to the acute exercise bout. Three additional groups of rats (n=6) were injected with either 8 mg/kg (i.p.) of lipopolysaccharide (LPS), 1 mmol/kg (i.p.) t-butylhydroperoxide (tBHP), or saline (C) and killed at resting condition. Ex rats showed higher levels of NF-kappaB b…

Lipopolysaccharidesmedicine.medical_specialtyP50PyrrolidinesElectrophoretic Mobility Shift AssayIκB kinaseBiologyProtein Serine-Threonine Kinasesmedicine.disease_causeBiochemistryRats Sprague-Dawleychemistry.chemical_compoundPyrrolidine dithiocarbamateNF-KappaB Inhibitor alphatert-ButylhydroperoxideThiocarbamatesInternal medicinePhysical Conditioning AnimalGeneticsmedicineAnimalsMuscle SkeletalMolecular Biologychemistry.chemical_classificationReactive oxygen speciesNF-kappa BSkeletal muscleI-kappa B KinaseRatsCytosolOxidative Stressmedicine.anatomical_structureEndocrinologychemistryFemaleI-kappa B ProteinsSignal transductionOxidative stressBiotechnologySignal TransductionFASEB journal : official publication of the Federation of American Societies for Experimental Biology
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Reversibly stable thiopolyplexes for intracellular delivery of genes.

2006

Novel polyaspartamide non-viral carriers for gene therapy were synthesized by introducing, on the same polymer backbone, positively charged groups, for electrostatic interactions with DNA, and thiol groups for the formation of disulfide bridges between polymer chains. The introduction of thiols was aimed to have a vector with low redox potential sensitivity: disulfide crosslinking in fact, being stable in extracellular environment, allowed either to have stable complexes in plasma, that can protect DNA from metabolism, or to be reduced inside the cell, where the excess of glutathion in reduced form maintains a low redox potential. The consequent destabilization of the complex after disulfid…

Magnetic Resonance SpectroscopyLightStereochemistryCell SurvivalPolymersPharmaceutical ScienceElectrophoretic Mobility Shift AssayGene deliveryTransfectionchemistry.chemical_compoundGene DeliveryMiceDynamic light scatteringGenes ReporterCell Line TumorAnimalsScattering RadiationElectrophoretic mobility shift assayDisulfidesSulfhydryl CompoundsLuciferaseschemistry.chemical_classificationthiopolycationsEndodeoxyribonucleasesLuminescent AgentsGenetic transferCationic polymerizationProteinsDNAChromatography Ion ExchangeCombinatorial chemistrychemistrypolyaspartammideAgarose gel electrophoresisThiolPeptidesOxidation-ReductionDNAJournal of controlled release : official journal of the Controlled Release Society
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Transcription of the MAT2A gene, coding for methionine adenosyltransferase, is up-regulated by E2F and Sp1 at a chromatin level during proliferation …

2006

Methionine adenosyltransferase (MAT) is an essential enzyme because it catalyzes the formation of S-adenosylmethionine, the main methyl donor. Two MAT-encoding genes (MAT1A, MAT2A) are found in mammals. The latter is expressed in proliferating liver, dedifferentiation and cancer, whereas MAT1A is expressed in adult quiescent hepatocytes. Here, we report studies on the molecular mechanisms controlling the induction of MAT2A in regenerating rat liver and in proliferating hepatocytes. The MAT2A is up-regulated at two discrete moments during liver regeneration, as confirmed by RNApol-ChIP analysis. The first one coincides with hepatocyte priming (i.e. G0-G1 transition), while the second one tak…

MaleChromatin ImmunoprecipitationTranscription GeneticSp1 Transcription FactorMolecular Sequence DataOligonucleotidesElectrophoretic Mobility Shift AssayBiologyBiochemistryS PhaseSequence Homology Nucleic AcidmedicineAnimalsE2F1Electrophoretic mobility shift assayRats WistarPromoter Regions GeneticE2FE2F4Cells CulturedCell ProliferationSp1 transcription factorBase SequenceG1 PhaseMethionine AdenosyltransferaseCell BiologyMolecular biologyChromatinLiver regenerationE2F Transcription FactorsLiver RegenerationRatsUp-Regulationmedicine.anatomical_structureLiverMethionine AdenosyltransferaseHepatocyteHepatocytesProtein BindingThe International Journal of Biochemistry & Cell Biology
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Differential regulation of interleukin-10 production by genetic and environmental factors – a twin study

2002

Interleukin-10 (IL-10) has a critical role in the regulation of immune responses. The relative contribution of genetic and environmental factors to IL-10 production is under debate. We performed a twin study in 246 monozygotic and dizygotic twins to assess the heritability of IL-10 production after LPS stimulation in whole blood. In addition, the influence of promoter single nucleotide polymorphisms (-1082, -819 and -592) on transcriptional activity and their binding to nuclear factors was studied in luciferase reporter gene and electrophoretic mobility shift assays. IL-10 production showed a genetic determination with a heritability of 0.5. Decreasing body mass index (BMI), smoking and fem…

MaleImmunologyElectrophoretic Mobility Shift AssaySingle-nucleotide polymorphismIn Vitro TechniquesBiologyPolymorphism Single NucleotideMonocytesCell LineGenes ReporterPolymorphism (computer science)GeneticsHumansAllelePromoter Regions GeneticTranscription factorGenetics (clinical)GeneticsBinding SitesHaplotypeDNAHeritabilityTwin studyInterleukin-10Interleukin 10Gene Expression RegulationTwin Studies as TopicFemaleTranscription FactorsGenes & Immunity
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Electrophoretically deposited α-Fe2O3 and TiO2 composite anchored on rGO with excellent cycle performance as anode for lithium ion batteries

2018

Abstract Two nanostructured oxides, α-Fe2O3 and TiO2 with a particle diameters 50 nm and 21 nm, were mixed with graphene oxide (GO). Composite thin films on a stainless steel substrate were obtained by electrophoretic deposition (EPD) procedure from water suspensions: α-Fe2O3/GO, TiO2/GO and α-Fe2O3/TiO2/GO. Subsequently reduction of as-prepared thin films was performed. Thicknesses of acquired films were evaluated in the range of 2–6 μm. Structure and morphology were investigated as well as electrochemical properties of all samples were studied. The results revealed that α-Fe2O3/TiO2/rGO (in this article denoted as FTGO) exhibited the specific discharge capacity of 790 mAh·g−1 after 150 cy…

Materials scienceGrapheneComposite numberOxidechemistry.chemical_element02 engineering and technologyGeneral Chemistry010402 general chemistry021001 nanoscience & nanotechnologyCondensed Matter Physics01 natural sciences0104 chemical sciencesAnodelaw.inventionchemistry.chemical_compoundElectrophoretic depositionChemical engineeringchemistrylawGeneral Materials ScienceLithiumThin film0210 nano-technologyFaraday efficiencySolid State Ionics
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Electrophoretic Nanocrystalline Graphene Film Electrode for Lithium Ion Battery

2015

Graphene sheets were fabricated by electrophoretic deposition method from water suspension of graphene oxide followed by thermal reduction. The formation of nanocrystalline graphene sheets has been confirmed by scanning electron microscopy, X-ray diffraction and Raman spectroscopy. The electrochemical performance of graphene sheets as anode material for lithium ion batteries was evaluated by cycling voltammetry, galvanostatic charge-discharge cycling, and electrochemical impedance spectroscopy. Fabricated graphene sheets exhibited high discharge capacity of about 1120 mAhg−1 and demonstrated good reversibility of lithium intercalation and deintercalation in graphene sheet film with capacity…

Materials scienceGrapheneGraphene foamInorganic chemistrychemistry.chemical_elementNanocrystalline materialLithium-ion batterylaw.inventionElectrophoretic depositionchemistryChemical engineeringlawLithiumGraphene nanoribbonsGraphene oxide paperIOP Conference Series: Materials Science and Engineering
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Laser densification of organic coating: Effects of laser wavelength, operating parameters and substrate properties

2012

International audience; Mechanical bonding and interface behaviour play a key role for any materials deposited on different substrates. Usually, a post-spray heat treatment is required to improve the coating morphology and to enhance mechanical properties of thermal-sprayed polymeric coating. The effects of YAG, CO2 and diode laser radiations on as-sprayed PEEK coating deposited on stainless steel and aluminum substrates were investigated. The results revealed a good coating densification and interface behavior. A correlation between coating and substrate absorption coefficients, their thermophysical properties and laser operating parameters was shown. Besides, the finite element modeling b…

Materials sciencePOLYETHERETHERKETONE PEEKchemistry.chemical_element02 engineering and technologySubstrate (electronics)engineering.materialADHESIONFILMSlaw.inventionElectrophoretic deposition0203 mechanical engineeringCoatinglawAluminiumELECTROPHORETIC DEPOSITIONMaterials ChemistryPeekComposite materialAbsorption (electromagnetic radiation)PEEK COATINGSDRY SLIDING FRICTIONSurfaces and InterfacesGeneral ChemistryMECHANICAL-PROPERTIES[SPI.MECA]Engineering Sciences [physics]/Mechanics [physics.med-ph]021001 nanoscience & nanotechnologyCondensed Matter PhysicsLaserSTAINLESS-STEELSurfaces Coatings and Films020303 mechanical engineering & transportschemistryCOMPOSITE COATINGSMelting pointengineering0210 nano-technologyBEHAVIOR
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Inkjet Printable ZnO/PEDOT:PSS Heterojunction for Thin Flexible Semi-Transparent Optoelectronic Sensors

2020

International audience; Flexible sensors play an increasing role in printed electronics and are of interest for optoelectronic applications in flexible robotics and industrial automation. Thus, we have investigated the hybrid inorganic-organic junction between ZnO and PEDOT:PSS (poly(3,4-ethylenedioxythiophene) polystyrene sulfonate). A thin ITO (indium tin oxide) layer on PET (polyethylene terephthalate) foils was used as substrate electrode. ZnO was deposited from a nanoparticle (NP) suspension by electrophoretic deposition. For comparison, we have used three different methods for the deposition of PEDOT:PSS, namely (i) drop casting, (ii) dip-coating, (iii) inkjet printing. For the result…

Materials scienceheterojunctionResistanceII-VI semiconductor materialsNanoparticle02 engineering and technologySubstrate (electronics)01 natural sciencesElectrophoretic depositionPEDOT:PSS0103 physical sciencesZinc oxide[CHIM]Chemical SciencesFilms010302 applied physicsphotosensorinkjet printingnegative photoresponseNanocompositebusiness.industry021001 nanoscience & nanotechnologyIndium tin oxideelectrophoretic depositionPrinted electronicsHeterojunctionsOptoelectronicsPrintingprinted electronics0210 nano-technologybusinessLayer (electronics)
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Theoretical and experimental study of the kinetics of particle chains near electrodes in dielectrophoretic devices

2014

Using a three dimensional coupled Monte Carlo-Poisson algorithm and experimental results we studied the role of the particle-particle dipole interaction on the kinetics of a system of human cells suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. We found that the kinetics of the cells during negative/repulsive dielectrophoresis depends on the local distribution of particles. If the cells have generated long chains during positive/attractive dielectrophoresis, such chains can hinder subsequent detachment resulting in a reduction of the separation/manipulation efficiency of dielectrophoretic device. This effec…

Materials sciencekinetics of particle chains dielectrophoretic devicesparticle chainsKineticsElectrodeDielectrophoresisAnalytical chemistrycellsParticle chainsNanotechnologyDielectrophoresis
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