Search results for "physiologic"

showing 10 items of 2593 documents

The shell matrix of the european thorny oyster, Spondylus gaederopus: microstructural and molecular characterization.

2020

17 pages; International audience; Molluscs, the largest marine phylum, display extraordinary shell diversity and sophisticated biomineral architectures. However, mineral-associated biomolecules involved in biomineralization are still poorly characterised.We report the first comprehensive structural and biomolecular study of Spondylus gaederopus, a pectinoid bivalve with a peculiar shell texture. Used since prehistoric times, this is the best-known shell of Europe’s cultural heritage. We find that Spondylus microstructure is very poor in mineral-bound organics, which are mostly intercrystalline and concentrated at the interface between structural layers.Using high-resolution liquid chromatog…

BiomineralizationProteomicsProteomeEvolution[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM]Shell (structure)Proteomics03 medical and health sciencesCalcification PhysiologicAnimal ShellsStructural BiologyAnimals14. Life underwater030304 developmental biologyMinerals0303 health sciencesbiology[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM]PhylumChemistry030302 biochemistry & molecular biologybiology.organism_classificationOstreidaeBiomineralization; Evolution; Liquid chromatography-tandem mass spectrometry; Proteomics; Shell biochemistryCharacterization (materials science)[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate ZoologySpondylusEvolutionary biologyLiquid chromatography-tandem mass spectrometryProteomeShell biochemistrySpondylus gaederopus[SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate ZoologyBiomineralization
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Shell proteome of rhynchonelliform brachiopods.

2015

7 pages; International audience; Brachiopods are a phylum of marine invertebrates that have an external bivalved shell to protect their living tissues. With few exceptions, this biomineralized structure is composed of calcite, mixed together with a minor organic fraction, comprising secreted proteins that become occluded in the shell structure, once formed. This organic matrix is thought to display several functions, in particular, to control mineral deposition and to regulate crystallite shapes. Thus, identifying the primary structure of matrix proteins is a prerequisite for generating bioinspired materials with tailored properties. In this study, we employed a proteomic approach to identi…

BiomineralizationProteomicsProteomeShell (structure)BrachiopodsBiologyMatrix (biology)ProteomicsCalcium CarbonatePaleontologychemistry.chemical_compoundCalcification PhysiologicAnimal ShellsStructural Biology[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ShellAnimals14. Life underwater[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsCalciteMineralsPhylumMarine invertebratesExtracellular matrix[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/BiomaterialsInvertebrateschemistryEvolutionary biology[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ProteomePeptidesBiomineralization
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Bioelectrical model of head-tail patterning based on cell ion channels and intercellular gap junctions

2020

Robust control of anterior-posterior axial patterning during regeneration is mediated by bioelectric signaling. However, a number of systems-level properties of bioelectrochemical circuits, including stochastic outcomes such as seen in permanently de-stabilized "cryptic" flatworms, are not completely understood. We present a bioelectrical model for head-tail patterning that combines single-cell characteristics such as membrane ion channels with multicellular community effects via voltage-gated gap junctions. It complements the biochemically-focused models by describing the effects of intercellular electrochemical coupling, cutting plane, and gap junction blocking of the multicellular ensemb…

BioquímicaTailPolarity (physics)Cèl·lulesBiophysicsHead-tail patterning02 engineering and technology01 natural sciencesIon ChannelsGap junctional communicationElectrochemistryAnimalsRegenerationPhysical and Theoretical ChemistryIon channelBody PatterningPhysicsbiologyRegeneration (biology)010401 analytical chemistryGap junctionGap JunctionsPlanariansGeneral Medicine021001 nanoscience & nanotechnologybiology.organism_classificationElectrophysiological Phenomena0104 chemical sciencesCoupling (electronics)Multicellular organismBioelectricityPlanarianBiophysicsPositional information0210 nano-technologyIon channelHeadIntracellular
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Assessing autophagy in archived tissue or how to capture autophagic flux from a tissue snapshot

2020

This article belongs to the Special Issue Autophagy in Cancer.

Bioquímicaautophagy:Ciências da Saúde [Ciências Médicas]Ciências Médicas::Ciências da SaúdeCellular homeostasisAutofagia610 Medicine & healthBiologyGeneral Biochemistry Genetics and Molecular Biology:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]03 medical and health sciences0302 clinical medicineHuman disease:Chemicals and Drugs::Biological Factors::Biological Markers [Medical Subject Headings]:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Autophagy [Medical Subject Headings]lcsh:QH301-705.5030304 developmental biology0303 health sciencesdiseaseBiología molecularScience & TechnologyGeneral Immunology and MicrobiologyMechanism (biology)CommunicationAutophagyautophagy; biomarkers; pathology; diseasebiomarkersPatología3. Good healthCell biology:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results [Medical Subject Headings]BiomarcadoresTejidoslcsh:Biology (General)030220 oncology & carcinogenesis570 Life sciences; biologypathologyGeneral Agricultural and Biological SciencesFlux (metabolism)Enfermedad:Phenomena and Processes::Physiological Phenomena::Physiological Processes::Homeostasis [Medical Subject Headings]
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Host–virus evolutionary dynamics with specialist and generalist infection strategies: Bifurcations, bistability, and chaos

2019

In this work, we have investigated the evolutionary dynamics of a generalist pathogen, e.g., a virus population, that evolves toward specialization in an environment with multiple host types. We have particularly explored under which conditions generalist viral strains may rise in frequency and coexist with specialist strains or even dominate the population. By means of a nonlinear mathematical model and bifurcation analysis, we have determined the theoretical conditions for stability of nine identified equilibria and provided biological interpretation in terms of the infection rates for the viral specialist and generalist strains. By means of a stability diagram, we identified stable fixed…

BistabilityPopulationGeneral Physics and AstronomyDynamical Systems (math.DS)Fixed pointParameter spaceBiologyGeneralist and specialist speciesModels Biological01 natural sciencesStability (probability)010305 fluids & plasmas0103 physical sciencesFOS: MathematicsHumansQuantitative Biology::Populations and EvolutionComputer SimulationMathematics - Dynamical SystemsQuantitative Biology - Populations and Evolution010306 general physicsEvolutionary dynamicseducationMathematical Physicseducation.field_of_studyApplied MathematicsDegenerate energy levelsPopulations and Evolution (q-bio.PE)Statistical and Nonlinear Physics3. Good healthNonlinear DynamicsEvolutionary biologyFOS: Biological sciencesHost-Pathogen InteractionsVirusesVirus Physiological Phenomena
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Bivariate nonlinear prediction to quantify the strength of complex dynamical interactions in short-term cardiovascular variability.

2005

A nonlinear prediction method for investigating the dynamic interdependence between short length time series is presented. The method is a generalization to bivariate prediction of the univariate approach based on nearest neighbor local linear approximation. Given the input and output series x and y, the relationship between a pattern of samples of x and a synchronous sample of y was approximated with a linear polynomial whose coefficients were estimated from an equation system including the nearest neighbor patterns in x and the corresponding samples in y. To avoid overfitting and waste of data, the training and testing stages of the prediction were designed through a specific out-of-sampl…

Bivariate time seriePhysics::Medical PhysicsBiomedical EngineeringBlood PressureBivariate analysisOverfittingCross-validationk-nearest neighbors algorithmCardiovascular Physiological PhenomenaHealth Information ManagementHeart RateTilt-Table TestStatisticsApplied mathematicsHumansComputer SimulationPredictabilityHeart rate variabilityMathematicsHealth InformaticBaroreflex controlSystolic arterial pressure variabilityUnivariateModels CardiovascularNonlinear predictionComputer Science Applications1707 Computer Vision and Pattern RecognitionComputer Science ApplicationsNonlinear systemComputational Theory and MathematicsNonlinear DynamicsLinear approximationMedicalbiological engineeringcomputing
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Physiological and agonistic behavioural response of Procambarus clarkii to an acoustic stimulus

2012

Summary This study examined the effects of an acoustic stimulus on the haemolymph and agonistic behaviour of the red swamp crayfish Procambarus clarkii. The experiment was conducted in a tank equipped with a video recording system using 6 groups (3 control and 3 test groups) of five adult crayfish (30 specimens in total). After one hour of habituation, the behaviour of the crayfish was monitored for two hours. During the second hour, the animals in the test groups were exposed to a linear sweep (frequency range 0.1-25 kHz; peak amplitude 148 dBrms re 1 µPa at 12 kHz) acoustic stimulus for 30 minutes. Exposure to the noise produced significant variations in haemato-immunological parameters a…

Blood GlucoseFish ProteinsMaleHemocytesSound SpectrographyPhysiologyVideo RecordingCell CountAstacoideaAquatic ScienceStimulus (physiology)Animal scienceHemolymphAgonistic behaviourAnimalsHSP70 Heat-Shock ProteinsHabituationMolecular BiologyEcology Evolution Behavior and SystematicsVideo recordingProcambarus clarkiibiologyHemagglutinationOsmolar ConcentrationAnatomyacoustic stress agonistic behaviour physiological response red swamp crayfish.biology.organism_classificationCrayfishAcoustic StimulationInsect ScienceFemaleAnimal Science and ZoologyRabbitsAgonistic BehaviorJournal of Experimental Biology
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Differential expression of the corticosteroid receptors GR1, GR2 and MR in rainbow trout organs with slow release cortisol implants

2012

The present study describes the transcriptional levels of the corticosteroid receptors (CRs) GR1, GR2 and MR in the different organs of the rainbow trout (Oncorhynchus mykiss) in response to a slow release of cortisol, throughout a 10-day period. We show that after short term (1 day after cortisol implantation), when the plasma levels of cortisol emulate an acute stress, the GR2 and MR expression levels were upregulated in the brain and head kidney tissues. This result reflects the role of these organs as regulators of the stress response. In general, the rest of the organs, especially gills, intestine, liver, muscle and spleen, showed decreased transcriptional levels of GR1, GR2 and MR, al…

Blood GlucoseFish Proteinsendocrine systemmedicine.medical_specialtyHydrocortisoneTranscription GeneticPhysiologymedicine.drug_classSpleenBiologyHematocritCarbohydrate metabolismBiochemistryReceptors GlucocorticoidDownregulation and upregulationStress PhysiologicalInternal medicinemedicineAnimalsChronic stressLactic AcidReceptorMolecular BiologyDrug Implantsmedicine.diagnostic_testOxygen transportBrainHead KidneyUp-RegulationReceptors Mineralocorticoidmedicine.anatomical_structureEndocrinologyOrgan SpecificityOncorhynchus mykissCorticosteroidhormones hormone substitutes and hormone antagonistsComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
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Profiling the physiological and molecular response to sulfonamidic drag in Procambarus clarkii

2014

Sulfamethoxazole (SMZ) is one of the most widely employed sulfonamides. Because of the widespread use of SMZ, a considerable amount is indeed expected to be introduced into the environment. The cytotoxicity of SMZ relies mainly on arylhydroxylamine metabolites (S-NOH) of SMZ and it is associated with the production of reactive oxygen species (ROS). There is limited information about the toxic potential of SMZ at the cellular and molecular levels, especially in aquatic and/or non-target organisms. In the present study, the red swamp crayfish (Procambarus clarkii), being tolerant to extreme environmental conditions and resistant to disease, was used as a model organism to profile the molecula…

Blood GlucoseGillsHemocytesAntioxidantSulfamethoxazolePhysiologyHealth Toxicology and Mutagenesismedicine.medical_treatmentHepatopancreasHaemolymphatic parametersAquacultureAstacoideaToxicologyBiochemistryArthropod ProteinsAnti-Infective AgentsRed swamp crayfishStress PhysiologicalGene expressionmedicineAnimalsMetallothioneinHSP70 Heat-Shock ProteinsTissue DistributionAntiossidanti enzymesharmony patio parameters proinflammatory genes red swap crac fish sulfametoxazoleProcambarus clarkiichemistry.chemical_classificationReactive oxygen speciesbiologyGene Expression Regulation DevelopmentalProinflammatory genesCell BiologyGeneral Medicinebiology.organism_classificationBlood Cell CountHsp70FerritinBiochemistrychemistryMolecular ResponseFerritinsbiology.proteinMetallothioneinAntioxidant enzymesOxidoreductasesBiomarkersWater Pollutants Chemical
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Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit

2017

Metabolic syndrome (MetS) has become one of the main concerns for public health because of its link to cardiovascular disease. Murine models have been used to study the effect of MetS on the cardiovascular system, but they have limitations for studying cardiac electrophysiology. In contrast, the rabbit cardiac electrophysiology is similar to human, but a detailed characterization of the different components of MetS in this animal is still needed. Our objective was to develop and characterize a diet-induced experimental model of MetS that allows the study of cardiovascular remodeling and arrhythmogenesis. Male NZW rabbits were assigned to control (n = 15) or MetS group (n = 16), fed during 2…

Blood GlucoseMale0301 basic medicinePhysiologylcsh:MedicineBlood Pressure030204 cardiovascular system & hematologyVascular MedicineBiochemistryEatingchemistry.chemical_compound0302 clinical medicineGlucose MetabolismDietary SucroseBlood plasmaMedicine and Health Scienceslcsh:ScienceMammalsMetabolic SyndromeMultidisciplinaryLiver DiseasesFatty liverAnimal ModelsBody FluidsBloodExperimental Organism SystemsPhysiological ParametersLiverVertebratesHypertensionMetabolomeCarbohydrate MetabolismRabbitsAnatomyResearch Articlemedicine.medical_specialtyMean arterial pressureBilirubinDiastoleGastroenterology and HepatologyBiologyResearch and Analysis MethodsDiet High-FatBlood Plasma03 medical and health sciencesInternal medicineGlucose IntolerancemedicineAnimalsMetabolomicsObesityNuclear Magnetic Resonance BiomolecularNutritionAnalysis of VarianceBody Weightlcsh:ROrganismsBiology and Life Sciencesmedicine.diseaseDietFatty LiverDisease Models AnimalMetabolism030104 developmental biologyEndocrinologyBlood pressurechemistryAmnioteslcsh:QMetabolic syndromeSteatosisPLOS ONE
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