Search results for "pig"
showing 10 items of 2235 documents
Highly Fluorescent and Water-Soluble Diketopyrrolopyrrole Dyes for Bioconjugation
2015
International audience; The preparation of highly water-soluble and strongly fluorescent diketopyrrolopyrrole (DPP) dyes using an unusual taurine-like sulfonated linker has been achieved. Exchanging a phenyl for a thienyl substituent shifts the emission wavelength to near λ=600 nm. The free carboxylic acid group present in these new derivatives was readily activated and the dyes were subsequently covalently linked to a model protein (bovine serum albumin; BSA). The bioconjugates were characterized by electronic absorption, fluorescence spectroscopy and MALDI-TOF mass spectrometry, thus enabling precise determination of the labeling density (ratio DPP/BSA about 3 to 8). Outstanding values of…
Phylostratic Shift of Whole-Genome Duplications in Normal Mammalian Tissues towards Unicellularity Is Driven by Developmental Bivalent Genes and Reve…
2020
Tumours were recently revealed to undergo a phylostratic and phenotypic shift to unicellularity. As well, aggressive tumours are characterized by an increased proportion of polyploid cells. In order to investigate a possible shared causation of these two features, we performed a comparative phylostratigraphic analysis of ploidy-related genes, obtained from transcriptomic data for polyploid and diploid human and mouse tissues using pairwise cross-species transcriptome comparison and principal component analysis. Our results indicate that polyploidy shifts the evolutionary age balance of the expressed genes from the late metazoan phylostrata towards the upregulation of unicellular and early m…
Molecular diagnosis and therapy of hepatocellular carcinoma (HCC): an emerging field for advanced technologies.
2011
Despite great progress in diagnosis and management of hepatocellular carcinoma (HCC), the exact biology of the tumor remains poorly understood overall limiting the patients' outcome. Detailed analysis and characterization of the molecular mechanisms and subsequently individual prediction of corresponding prognostic traits would revolutionize both diagnosis and treatment of HCC and is the key goal of modern personalized medicine. Over the recent years systematic approaches for the analysis of whole tumor genomes and transcriptomes as well as epigenomes became affordable tools in translational research. This includes simultaneous analyses of thousands of molecular targets using microarray-bas…
Structural and Mechanical Characteristics of the Heart of the Icefish Chionodraco hamatus (Lönnberg)
1991
The absence of haemoglobin from the blood of icefish, antarctic teleosts of the family Chaenichthydae, has stimulated important physiological mechanisms which serve to compensate for the absence of the respiratory pigment. In view of the central role played by the circulation in meeting the oxygen demands of the tissues, it is not surprising to find that the cardiocirculatory system has been extensively remodelled in icefish. These adaptations include: (1) increases in blood volume, from two to four times larger than that in many teleosts (Hemmingsen and Douglas 1970; Holeton 1970); (2) increases in blood flow associated with lowered oxygen demands (Hemmingsen and Douglas 1970; Holeton 1970…
Prediction of CRT Activation Sequence by Personalization of Biventricular Models from Electroanatomical Maps
2020
[EN] Optimization of lead placement and interventricular delay settings in patients under cardiac resynchronization therapy is a complex task that might benefit from prior information based on models. Biophysical models can be used to predict the sequence of electrical heart activation in a patient given a set of parameters which should be personalized to the patient. In this paper, we use electroanatomical maps to personalize the endocardial activation of the right ventricle, and the different tissue conductivities in a pig model with left bundle branch block, to reproduce personalized biventricular activations. Following, we tested the personalized heart model by virtually simulating card…
Can DNA methylation contribute to explain morphological variability? The case of loggerhead turtle Caretta caretta
2015
The loggerhead turtle Caretta caretta is a common and threatened sea turtle in the Mediterranean Sea. It is listed in the Red List of Threatened Species of the International Union for Conservation of Nature (IUCN 2012) and, although it is widely studied, little is currently known about the relationships between its phenotype and genetic variability. In the last few years some authors observed the presence of individuals, collected in different parts of the Mediterranean Sea, with a variable number of carapacial and plastron scutes. This variability, in some cases, makes difficult the sound identification of the species (Margaritoulis and Chiras, 2011; Turkozan et al. 2001; Oliver 2014). To …
The epitaph of the Citharoeda Jucunda (AE 2007, 805: Segobriga). New edition and commentary
2015
Nueva edición y comentario filológico del epitafio en verso de una joven esclava, cuya habilidad musical se ensalza tanto en el retrato en relieve que adorna el monumento (una estela) como en el texto. Se trata de un epigrama en dísticos elegíacos, precedido de un monóstico (un hexámetro dactílico) que debe entenderse como una sentencia sobre la immatura mors. El anónimo autor de la inscripción se revela como representante de una tradición epigramática culta. Los numerosos loci similes evidencian, sobre todo, la influencia de Ovidio, pero también la de Marcial y, probablemente, la de Juvenal. New edition and philological commentary about an epitaph in verse of a young slave. The monument (s…
Fabvla Nvlla Fvit. Annotations to a carmen Latinvm epigraphicvm, probably found in Gades
2017
Nueva lectura e interpretación de un carmen Latinum epigraphicum sepulcral, en el que hay una laudatio de la difunta por contraposición a la mítica Helena. New reading and interpretation of a sepulchral carmen Latinum epigraphicum with a laudatio of the deceased woman in contrast to the mythical Helen.
Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi.
2016
Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters…
Liriche ed epigrammi
2015
Edizione critica, con traduzione italiana e note di commento, delle Nugae e degli Epigrammata di Gaio Valerio Catullo