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showing 10 items of 706 documents
In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis
2003
Several clinical trials have revealed that individuals who were given beta-carotene and vitamin A did not have a reduced risk of cancer compared to those given placebo; rather, vitamin A could actually have caused an adverse effect in the lungs of smokers [Omenn, G.S., Goodman, G.E., Thornquist, M.D., Balmes, J., Cullen, M.R., Glass, A., Keogh, J.P., Meyskens, F.L., Valanis, B., Williams, J.H., Barnhart, S. & Hammar, S. N. Engl. J. Med (1996) 334, 1150-1155; Hennekens, C.H., Buring, J.E., Manson, J.E., Stampfer, M., Rosner, B., Cook, N.R., Belanger, C., LaMotte, F., Gaziano, J.M., Ridker, P.M., Willet, W. & Peto, R. (1996) N. Engl. J. Med. 334, 1145-1149]. Using differential display techniq…
Entrepreneurship Training and Self-Employment among University Graduates
2012
In economies characterized by low labor demand and high rates of youth unemployment, entrepreneurship training has the potential to enable youth to gain skills and create their own jobs. This paper presents experimental evidence on a new entrepreneurship track that provides business training and personalized coaching to university students in Tunisia. Undergraduates in the final year of licence appliquee were given the opportunity to graduate with a business plan instead of following the standard curriculum. This paper relies on randomized assignment of the entrepreneurship track to identify impacts on labor market outcomes one year after graduation. The analysis finds that the entrepreneur…
Identification of a gene overexpressed in aphids reared under short photoperiod.
2003
Most aphids develop a cyclic parthenogenesis life-cycle. After several generations of viviparously produced parthenogenetic females, follows a single annual generation of sexual individuals, usually in autumn, that mate and lay the sexual eggs. Shortening of photoperiod at the end of the summer (together with temperature) is a key factor inducing the sexual response. Currently no genes involved in the cascade of events that lead to the appearance of sexual forms have been reported. After a Differential Display RT-PCR survey performed on Acyrthosiphon pisum aphids, we identified a gene that is overexpressed in aphids reared under short photoperiod conditions that induce sexuality in this spe…
Role of ambient light in the detection of contrast elements in digital dental radiography.
2018
Objective This study aimed to evaluate the effect of different ambient light levels on observer detection of small contrast differences in a contrast phantom by using a high-end liquid crystal display (LCD) monitor. Study Design An aluminum step wedge was converted into a contrast phantom by the addition of bore holes. Radiographic images of the contrast elements were presented to 20 observers. Images were displayed in random order under different ambient light levels (0, 50, 200, and 500 lux) twice, and the observers had to determine if contrast elements were visible. Sensitivity and specificity were determined to calculate areas under receiver operating characteristic curves and Friedmann…
Un ejemplo de dinámica de role playing en una asignatura de libre configuración en Derecho
2011
Las exigencias del actual mundo laboral y profesional requieren una mayor creación de espacios de formación universitaria para la solución de problemas reales. El role playing, como técnica didáctica diseñada para asignaturas de libre configuración cursadas por estudiantes de diferente perfil formativo, facilita el proceso de enseñanza aprendizaje de contenidos complejos del Derecho. Este artículo trata de describir y evaluar la experiencia docente en una dinámica basada en la simulación para la asignatura “Convención sobre la eliminación de todas las formas de discriminación contra la mujer”.
PCR-Amplified cDNA Probes for Verification of Differentially Expressed Genes
1997
Differential display has proven to be a powerful technique for the detection and isolation of differentially expressed genes. By generating reproducible cDNA expression patterns, it is possible to compare gene expression by two or more cell types, developmental stages or tissues and to isolate as yet unknown differentially expressed genes. A sensitive method is necessary to verify the differential expression of the isolated cDNAs. Here we describe the use of adaptor-ligated, PCR-amplified total cDNA of the two cell types compared as a probe for Southern hybridizations with the isolated cDNAs.
Mid-region parathyroid hormone-related protein (PTHrP) and gene expression of MDA-MB231 breast cancer cells.
2007
Abstract We have previously shown that PTHrP(38–94) amide restrains growth and invasion in vitro, causes striking toxicity and accelerates death of some breast cancer cell lines, the most responsive being MDA-MB231, for which tumorigenesis was also attenuated in vivo. We have also demonstrated that mid-region PTHrP gains access to the nuclear compartment of these cells and displays DNA-binding properties in vitro by recognizing targets in both cellular chromatin and isolated oligonucleotides. Here, we examined whether PTHrP(38–94) amide was able to modulate gene expression of MDA-MB231 cells, employing a combination of conventional, differential display and semi-quantitative multiplex PCR t…
Induction of tcI 7 , a gene encoding a β-subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide 1
2000
We previously isolated, by differential display and 5′ RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for obacco ryptogein nduced), a gene encoding a β-subunit of proteasome. Here, we report that tcI 7 was up-regulated in tobacco plants treated with elicitins (cryptogein and parasiticein) that have been shown to induce a systemic acquired resistance (SAR). Moreover, subsequent inoculation of tobacco with the pathogen Phytophthora parasitica var. nicotianae (Ppn) was shown to induce an additional activation of tcI 7 in tobacco plants pretreated with cryptogein. We also showed an…
Group-level analysis on multiplayer game collaboration: how do the individuals shape the group interaction?
2010
In this study, the aim was to examine how small-group collaboration is shaped by individuals interacting in a virtual multiplayer game. The data were collected from a design experiment in which six randomly divided groups of four university students played a voice-enhanced game lasting about 1 h. The ‘eScape’ game was a social action adventure developed as a part of the study. In the analysis of the video data, students' discourse functions during the game were analysed with content analytic methods for studying the nature of their interaction. An effort was made to analyse the data on both group and individual levels, and therefore the participants' prior social ties and experience in gami…
Infiniviz: Taking Quake 3 Arena on a Large-Scale Display System to the Next Level
2018
The authors of this paper have previously presented a large-scale display system called Infiniviz in other publications. Infiniviz attempts to improve network bandwidth consumption and computational performance compared to other existing large-scale display systems. Since the previous publications have been made in early development stages of Infiniviz, only the overview of the software architecture and details of hardware implementation have been presented so far. This paper contains a real-life test of Infiniviz running Quake 3 Arena at a resolution of 9600 x 5400 at 24 fps. Also, in this paper, the authors have tried to match their results to what has been published by other researchers …