Search results for "porter"
showing 10 items of 920 documents
The Political Click: Political Participation through E-Petitions in Germany
2010
Electronic petitions can serve as an influential mechanism for political participation. We present a study on the dynamics in the German e-petition system which was introduced in late 2008. Drawing on a data set of signatures, we analyze four aspects: (a) the types of petitions found, (b) the temporal dynamics of petitions, (c) the types of users found, and (d) the intersection of different petitions' supporter populations. We present evidence that (a) the system is dominated by a very small number of high-volume petitions and (b) these high-volume petitions have a delayed boosting effect on the base activity in the petition system. We furthermore (c) present a typology of users, showing th…
von Hippel-Lindau Protein-Mediated Repression of Tumor Necrosis Factor Alpha Translation Revealed through Use of cDNA Arrays
2003
Based on evidence that the von Hippel-Lindau (VHL) tumor suppressor protein is associated with polysomes and interacts with translation regulatory factors, we set out to investigate the potential influence of pVHL on protein translation. To this end, renal cell carcinoma (RCC) cells that either lacked pVHL or expressed pVHL through stable transfection were used to prepare RNA from cytosolic (unbound) and polysome-bound fractions. Hybridization of cDNA arrays using RNA from each fraction revealed a subset of transcripts whose abundance in polysomes decreased when pVHL function was restored. The tumor necrosis factor alpha (TNF-alpha) mRNA was identified as one of the transcripts that prefere…
Specific sequence elements in the 5′ untranslated regions of rbcL and atpB gene mRNAs stabilize transcripts in the chloroplast of Chlamydomonas reinh…
2001
Using a series of point mutations in chimeric reporter gene constructs consisting of the 5' regions of the Chlamydomonas chloroplast rbcL or atpB genes fused 5' to the coding sequence of the bacterial uidA (GUS) gene, RNA-stabilizing sequence elements were identified in vivo in the 5' untranslated regions (5' UTRs) of transcripts of the chloroplast genes rbcL and atpB in Chlamydomonas reinhardtii. In chimeric rbcL 5' UTR:GUS transcripts, replacement of single nucleotides in the 10-nt sequence 5'-AUUUCCGGAC-3', extending from positions +38 to +47 relative to the transcripts' 5' terminus, shortened transcript longevity and led to a reduction in transcript abundance of more than 95%. A similar…
Changes in the 5?-untranslated region of the rbcL gene accelerate transcript degradation more than 50-fold in the chloroplast of Chlamydomonas reinha…
2003
Using uidA (beta-glucuronidase; GUS) reporter gene constructs, the 5'-untranslated region (UTR) of the Chlamydomonas chloroplast rbcL gene was screened by deletion and mutational analysis for the presence of a promoter element that previous studies implied to reside within the first 63 base pairs of the UTR. Deleting a large segment of the rbcL 5'UTR in a 3'--5' direction to position +36, changing the remaining 36 base pairs at the 5' end of the UTR, and increasing by five base pairs the distance between the rbcL 5'UTR and the basic promoter element located at position -10 did not abolish transcription from the basic rbcL promoter. It is concluded that the apparent loss of transcriptional a…
The 3'-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing …
2003
The expression of the major protein kinase C substrate MARCKS (myristoylated alanine-rich C kinase substrate) is controlled by the stability of its mRNA. While the MARCKS mRNA is long living in quiescent fibroblasts (t1/2 = 14 h), its half-life time is drastically reduced (t1/2 = 2 h) in cells treated with phorbol esters to activate protein kinase C (PKC) or treated with growth factors. In a first step to study the underlying mechanism we identified both a cis-element on the MARCKS mRNA and the corresponding trans-acting factors. Fusing the complete 3'-UTR or specific regions of the 3'-UTR of the MARCKS gene to a luciferase reporter gene caused a drastic decrease in luciferase expression to…
3'-Untranslated regions of oxidative phosphorylation mRNAs function in vivo, as enhancers of translation
2000
Recent findings have indicated that the 3´-untranslated region (3´-UTR) of the mRNA encoding the β-catalytic subunit of the mitochondrial H+-ATP synthase has an in vitro translation-enhancing activity (TEA) [Izquierdo and Cuezva, Mol. Cell. Biol. (1997) 17, 5255–5268; Izquierdo and Cuezva, Biochem. J. (2000) 346, 849–855]. In the present work, we have expressed chimaeric plasmids that encode mRNA variants of green fluorescent protein in normal rat kidney and liver clone 9 cells to determine whether the 3´-UTRs of nuclear-encoded mRNAs involved in the biogenesis of mitochondria have an intrinsic TEA. TEA is found in the 3´-UTR of the mRNAs encoding the α- and β-subunits of the rat H+-ATP syn…
Competitive advantage in a global market: The Case of the Norwegian Salmon Farming Industry A comparative study of the Chilean salmon farming industry
2018
Master's thesis Business Administration BE501 - University of Agder 2018 Purpose – This study tries to address the differentiation done by a mature industry as a comparative study to Felzensztein and Gimmon´s (2014) paper on the Chilean farmed salmon industry. It focuses on how firms might improve their performance in an international context. Choosing between either cost-advantage or differentiation as a form gaining a competitive advantage, this study further addresses the usage of the country of origin effect as a form of differentiation. Design/Methodology/Approach – The research unit of the thesis is set in the Norwegian salmon farming industry, alas the biggest producer of salmon in t…
The pea sulfate transporter, PsSULTR4, contributes to seed yield and quality
2023
To investigate the role of vacuolar sulfate in seed yield and quality, we have targeted the single pea SULTR4 gene (PsSULTR4), which encodes a transporter homologous to Arabidopsis SULTR4;1 and 4;2 that allow sulfate efflux from the vacuole to the cytosol. By simulating the 3D structure of PsSULTR4, we observed that it is similar to that of SULTR4;1 in Arabidopsis. Furthermore, a phylogenetic analysis revealed a high level of conservation of SULTR4 protein motifs across land species. A fluorescent protein fusion experiment confirmed that PsSULTR4 localizes to the vacuolar membrane.Five sultr4 mutants were identified by TILLING (Targeting Induced Local Lesions IN Genomes), two of which showe…
SIA "Topus Riga" uzņēmuma konkurētspējas paaugstināšanas iespējas
2016
Bakalaura darba tēma ir uzņēmuma SIA “Topus Riga” konkurētspējas paaugstināšanas iespējas. Autore izvēlējās šo tēmu, lai novērtētu uzņēmuma konkurētspēju un tās attīstības iespējas. Uzņēmuma konkurētspējas novērtējums un konkurētspējas paaugstināšanas plāns palīdzēs uzņēmumam piesaistīt lielāku klientu skaitu, noturēt esošos klientus un tā rezultātā palielināt ienākumus. Bakalaura darba mērķis: Izpētīt SIA “Topus Riga” konkurētspēju un izstrādāt konkurētspējas paaugstināšanas plānu. Bakalaura darba pirmajā nodaļā autore veikusi teorētisku konkurences un konkurētspējas konceptu analīzi, balstoties uz vadošo mārketinga jomā pētnieku atziņām. Otrajā nodaļā autore raksturojusi SIA “Topus Riga” …
Pakalpojumu uzņēmuma konkurētspēja, to ietekmējošie faktori
2017
Neskatoties uz to, ka SIA Ad Verbum uzņēmums savu saimniecisko darbību veic arvien lielākā apjomā un to, ka uzņēmuma stāvoklis kopumā ir stabils, ir būtiski noteikt iespējamās vājās puses, draudus un iespējas, kas jāizmanto ņemot vērā konkurences īpatnības un ārējās vides stāvokli. Pretējā gadījumā uzņēmums var ātri zaudēt savas iegūtās konkurētspējas priekšrocības. Bakalaura darba mērķis ir balstoties uz uzņēmuma konkurētspējas teorētisko aspektu analīzi noteikt uzņēmuma „Ad Verbum” konkurētspējas paaugstināšanas iespējas un izstrādāt priekšlikumus darbības pilnveidošanai. Bakalaura darba mērķa sasniegšanai izvirzīti sekojoši pētāmie uzdevumi: 1.Pētīt un analizēt ekonomisko un mārketinga l…