Search results for "potyvirus"
showing 7 items of 27 documents
Abscisic Acid Connects Phytohormone Signaling with RNA Metabolic Pathways and Promotes an Antiviral Response that Is Evaded by a Self-Controlled RNA …
2020
© 2020 The Authors.
Transmission of PPV-M to Prunus persica by Brachycaudus schwartzi and Phorodon Humuli (Hem. Aphididae)
2004
Aphids are reported to be vectors of the most serious viral pathogen of the drupaceous species plum pox virus (PPV), but there is little direct experimental evidence of this. PPV (serotype M) is widespread in peach orchards even where there are severe control measures. Laboratory bioassays were conducted to study, under controlled conditions, the ability of Brachycaudus schwartzi (Borner) and Phorodon humuli (Schrank) to transmit PPV (serotype M). The results have shown that all the peach trees tested had evident symptoms of sharka and were positive to the RT-PCR analysis, confirming the ability of these two aphid species to transmit the virus.
Obtention d'anticorps monoclonaux polyvalents utilisables pour la détection ELISA du Plum pox virus (PPV) en utilisant un peptide synthétique représe…
2005
National audience
Analysis of eIF4E gene sconfering resistance to Pea seed-borne mosaic virus (PSbMV) and other potyviruses in pea
2013
Pea seed-borne mosaic virus (PSbMV) belongs among the most frequent viral pathogens causing severe losses in field pea and other legumes. These losses might be prevented by growth of resistant varieties. Resistance to the common P1 strain of PSbMV isconferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus), while to lentil L1 strainis localized on LGII atsbm-2locus. Gene-specific single nucleotide polymorphism and co-dominant amplicon length polymorphism markers were developed. From the comparison of genotype verses phenotype for selection of resistance/susceptibility in F2 plants, there was a 26% discrepancy between the PCR and ELISA-based assays with potentially susce…
Evolución de virus en huéspedes con susceptibilidad variable: consecuencias en eficacia y virulencia
2020
Los virus de RNA se encuentran entre los patógenos emergentes más abundantes. Durante su ciclo de vida normal, los virus se mueven de un huésped a otro, a veces transmitidos por el aire o el agua, pero a veces utilizando vectores en los que también pueden replicarse. Dentro de un huésped individual, los virus se enfrentan a múltiples tejidos y tipos de células que difieren en propiedades fisiológicas y bioquímicas y son constantemente desafiados por una variedad de respuestas inmunes antivirales. Para maximizar su fitness, los virus deben adaptarse necesariamente a todos estos factores. En la mayoría de los casos, esta adaptación se produce fácilmente debido a su enorme potencial evolutivo.…
Production and characterization of the recombinant Sphingomonas chlorophenolica pentachlorophenol 4-monooxygenase.
2001
Abstract Pentachlorophenol 4-monooxygenase (PCP4MO) from Sphingomonas chlorophenolica is a flavoprotein that hydroxylates PCP in the presence of NADPH and oxygen. In order to investigate the structure and function of active site, recombinant PCP4MO (rePCP4MO) was produced in Escherichia coli as a glutathione S-transferase (GST) fusion protein. Moreover, a tobacco etch virus (TEV) protease cleavage site (EKLYFQG) was introduced into GST-PCP4MO and a his-tagged TEV protease was employed. Hence, a two-step purification protocol was developed which allowed obtaining 15–20 mg of rePCP4MO from 1 L culture. The rePCP4MO revealed identity with native enzyme by SDS–PAGE and N-terminal sequence analy…
WRKY gene family drives dormancy release in onion bulbs
2022
Onion (Allium cepa L.) is an important bulb crop grown worldwide. Dormancy in bulbous plants is an important physiological state mainly regulated by a complex gene network that determines a stop of vegetative growth during unfavorable seasons. Limited knowledge on the molecular mechanisms that regulate dormancy in onion were available until now. Here, a comparison between uninfected and onion yellow dwarf virus (OYDV)-infected onion bulbs highlighted an altered dormancy in the virus-infected plants, causing several symptoms, such as leaf striping, growth reduction, early bulb sprouting and rooting, as well as a lower abscisic acid (ABA) level at the start of dormancy. Furthermore, by compar…